CD BioGlyco has a professional technical platform and well-trained researchers to provide customers with comprehensive fatty acid analysis services. We have confidence to be your essential research assistant in the field of glycobiology.
Fatty acids (FAs) are a class of compounds composed of carbon, hydrogen and oxygen. They are the main components of neutral fats, phospholipids and glycolipids. They can be divided into different categories according to their carbon chain length or whether they contain unsaturated bonds. FAs in cells comes from exogenous or de novo synthesis pathway.
Lipids contain a large number of heterogeneous compounds, most of which contain FAs in their structure, making these compounds suitable tools to examine processes ranging from cellular to macroscopic levels of the organization. FAs function as part of molecules or alone, and have multiple functions in cells. They have the structural function as a component of phospholipids, and phospholipids are blocks of cell membranes. As a part of neutral lipids, FAs can be used as a storage material in cells and FA derivatives are related to cell signal transduction. FAs metabolism is important in many research fields, including biology, ecology, bacteriology, human nutrition and health. Specific FAs and their ratios in cell membranes can be used as biomarkers to research the adaptability of bacterial cells to toxic compounds and environmental conditions, and to reveal the connection between the food web.
At CD BioGlyco, we use the following methods to analyze fatty acids.
GC-MS: analyze fatty acids after converted into fatty acid methyl ester forms (FAME). The free fatty acids or esters (such as methyl, ethyl, butyl) are also analyzed by GC-MS. When only basic chain length and saturation information is needed, wax type capillary columns are used for separation. To quantify cis- and trans-isomerization, highly polar capillary column can be used.
Using base-mediated hydrolysis to remove O-linked fatty acids, and acid-mediated hydrolysis to remove all fatty acids, whether fatty acids are linked to saccharide through hydroxyl or amino groups can be detected.
CD BioGlyco has numerous successful experiences in fatty acid analysis. Our comprehensive services and powerful data analysis have won a lot of praise in the international arena. We will continue to raise the standard to meet customers' glycobiology research needs.
Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.