Overview of Cell-based DEGL Screening

Cell-based DNA-encoded glycan library (DEGL) screening is a technique that combines the principles of DNA-encoded libraries (DELs) with the screening of glycan structures directly on cell surfaces. The purpose of this method is to identify ligands or small molecules that interact specifically with glycan structures presented on cell membranes, which are often involved in critical biological processes and disease states.

Precision Screening for Glycan Insights, Directly from the Cell Surface

At CD BioGlyco, our cell-based DEGL screening service offers an approach to identifying glycan-protein interactions directly on living cells. In this service, a diverse library of glycan structures, each uniquely encoded with a DNA sequence, is introduced to living cells that express various glycan-binding proteins or receptors on their surfaces. The interactions between the glycans in the library and the cell surface proteins are then captured and analyzed. The DNA tags linked to the bound glycans are recovered, amplified, and sequenced, enabling precise identification of the glycan structures that interact with the target proteins on the cell surface. This method is particularly advantageous because it enables the exploration of glycan-protein interactions within a biologically relevant environment, where proteins maintain their native conformations and are naturally associated with the cell membrane. The detailed workflow of cell-based DEGL screening service follows a meticulous process designed to identify glycan-protein interactions on the surface of living cells, ensuring a biologically relevant context for discovering active ligands.

Workflow

Amplification and Sequencing

The recovered DNA tags are amplified using PCR and then subjected to next-generation sequencing (NGS).

Data Analysis

The sequencing data is analyzed using advanced computational tools, often incorporating machine learning algorithms, to filter out noise and identify true positive interactions.

Hit Validation

The most promising glycan structures are selected for further validation in additional biological assays to confirm their activity and specificity.

Applications

• Our cell-based DEGL screening is particularly valuable in identifying potential candidates that interact with specific glycan-binding proteins on cell surfaces.
• Our service can be used to study the functional roles of glycans in various biological processes.
• Our cell-based DEGL screening allows for the identification of glycan biomarkers directly from living cells, aiding in the development of more accurate diagnostic tools.

Advantages

• Our service allows for screening in a native cellular environment, where proteins retain their natural conformation and post-translational modifications.
• By combining the vast diversity of DEGL with the specificity of cell-based assays, the service provides a high-throughput platform that can efficiently identify specific glycan-protein interactions.
• Our service can be customized to target a wide range of cell surface proteins and glycan interactions, making it versatile for different research and development needs.

Publication

Frequently Asked Questions

• What is cell-based DEGL screening, and how does it differ from traditional screening methods?
  • Cell-based DEGL screening involves introducing a diverse library of DNA-tagged glycan structures to living cells that express specific glycan-binding proteins on their surfaces. Unlike traditional methods that use purified proteins, this approach screens glycan interactions in their native cellular environment, which helps identify biologically relevant ligands with greater accuracy.
• How is the data from cell-based DEGL screening analyzed?
  • After the screening process, the DNA tags linked to the bound glycans are recovered, amplified, and sequenced. Advanced data analysis techniques, including machine learning, are then applied to filter out noise and identify true positive interactions. This approach allows for the identification of structure-activity relationships (SARs) and ranks potential hits for further validation.

At CD BioGlyco, our cell-based DEGL screening service is an innovative platform designed to identify glycan-protein interactions with high specificity and relevance. Whether you're in drug discovery, functional glycomics, or biomarker research, our service ensures precise and efficient identification of potential therapeutic targets, driving forward the future of glycan-related research. Please feel free to contact us for more information and please do not hesitate to discover how we can support your research.

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