Liquid Phase-based DNA-encoded Glycan Library (DEGL)
Overview of Liquid Phase-based DNA-encoded Glycan Library (DEGL)
DEGL is full of richness and diversity with the continuous development of glycobiology technology. CD BioGlyco provides comprehensive DEGL Construction solutions such as Solid Phase-based DEGL and liquid phase-based DEGL. Our researchers strive to diversify the chemical space of the encoded libraries by improving the efficiency of glycan library synthesis through new synthetic methods and technological advances. We would like to take you on a journey to explore the mysteries of glycan libraries!
Your Trusted Liquid Phase-based DEGL Construction Partner
Depending on the client's needs, CD BioGlyco provides structurally diverse, high-quality glycan library construction services and also ensures DNA-compatible reaction conditions.
Liquid-liquid Two-phase Flow Reaction System Design
- Relying on a robust flow system, our researchers use amide bond formation on a DNA-tagged amine to construct DEGL in flow. Our researchers transfer the amide coupling reaction on a DNA-tagged amine to flow chemistry.
- The liquid-liquid biphasic contains a dispersed phase and a continuous phase. We offer liquid-liquid biphasic optimization of DNA-tagged substrates. Our lab controls the flow state by regulating the fluid velocity.
- Depending on the needs of the DNA encoding substrate, our researchers provide a stable biphasic flow to ensure that the reaction mixture is separated individually.
- Our researchers systematically optimize different reaction conditions, including adjusting pH, temperature, and catalyst concentration.
- We offer a full range of validation services including conversion analysis, amide coupling reaction utility testing, structure, and purity of amide coupling products. We provide reversed-phase high-performance liquid chromatography (RP-HPLC) purification and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis.
Workflow
Combining microfluidic reactor technology and flow chemistry, our researchers provide high-quality liquid phase-based DEGL. Our experienced experts plan the entire project for you including the design of the liquid phase system, selection of chemical reaction type, coding sequence design, conjugates purification and analysis, transfer of DNA-encoded amide coupling, library construction, and process optimization.
Applications
- Liquid phase-based DEGL can be an excellent tool for molecular target screening.
- Liquid phase-based DEGL provides a new pathway for glycan library synthesis and promotes the development of glycobiology-related libraries.
- Liquid phase-based DEGL can be used to screen for glycan-associated disease targets.
Advantages of Us
- The liquid-liquid two-phase flow reactors we provide enable parallel conduction of reactions on DNA-coupled substrates.
- Our researchers have extensive experience in liquid-phase synthesis library development. The services we provide show the advantages of automation, precise process control, and high activity.
- Our experts evaluate and analyze all test reports to ensure the most accurate data.
Publication Data
Technology: Liquid-liquid two-phase flow reaction, Flow chemistry, Ultraviolet-visible (UV-vis) absorption spectroscopy, MALDI-MS spectra
Journal: Reaction Chemistry & Engineering
Published: 2023
IF: 5.2
Results: Researchers have developed an efficient and stable two-phase flow reaction system for amide coupling in DNA-encoded chemical libraries to overcome the limitations of conventional methods in terms of efficiency and compatibility. The team optimized the reaction conditions, including solvent selection, reaction time, temperature, and the use of catalysts and reagents. The researchers used different fragments of DNA-encoded chemical libraries as substrates to validate the broad applicability and reaction efficiency of the system. This study demonstrates the potential of liquid-phase synthesis in DNA-encoded libraries
Fig.1 Schematic diagram of liquid-liquid two-phase reaction. (Dinter, et al., 2023)
Frequently Asked Questions
- What are the advantages of liquid phase-based DEGL?
- Efficiency and accuracy: Liquid-phase synthesis is typically performed in solution, and our researchers utilize known chemical reactions to perform multi-step synthesis under precise control.
- Many two-phase liquid-phase synthesis reactions can be carried out under physiological conditions, which is particularly important for the synthesis of biologically active molecules.
- Automation and high-throughput screening: Liquid phase synthesis methods can be used in conjunction with advanced automation devices, and large-scale synthesis and screening processes can be automated via robotic platforms, thereby improving overall experimental efficiency and data consistency.
- What is DEGL?
- DEGL utilizes DNA as an identity marker to link each glycan to a unique DNA sequence, creating a large library of screenable glycans. It can be used to efficiently screen and identify glycan molecules that have specific interactions with particular biological targets (e.g., proteins, cells, tissues, etc.).
CD BioGlyco provides efficient and automated liquid phase-based DEGL construct services to clients. Our staff adheres to a strict system of processes and quality control to ensure optimal delivery. If you are interested in the glycan library, please feel free to contact us for more details.
Reference
- Dinter, R.; et al. Development of a two-phase flow reaction system for DNA-encoded amide coupling. Reaction Chemistry & Engineering. 2023, 8(6): 1334-1340.
For research use only. Not intended for any clinical use.
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