Overview of DNA-encoded Glycan Allosteric Inhibitor Library

The DNA-encoded glycan allosteric inhibitor library is a novel biotechnological tool for screening glycan allosteric inhibitors. The library combines the unique advantages of DNA-encoded Glycan Library (DEGL) technology and glycochemistry to encode diverse glycan compounds into DNA sequences for high-throughput and efficient screening of glycan allosteric inhibitors. Compared with traditional screening methods, the DNA encoding strategy significantly improves the screening efficiency and hit rate of glycan modification inhibitors and shortens the time required for experiments.

As a professional company engaged in research on glycobiology, CD BioGlyco provides personalized DNA-encoded glycan allosteric inhibitor library construction services to clients from various industries around the world. Our services cover the entire process from the design of DNA-encoded fragments to high-throughput screening.

Unlock Potential with DNA-Encoded Glycan Allosteric Inhibitors

Synthesis and Linkage of DNA Sequences

Based on the client's research needs, we add the universal sequence at both ends of the DNA sequence to be synthesized as the target DNA sequence, and design and synthesize the DNA strand used to encode the glycan according to this sequence. Next, we amplify the sequences by polymerase chain reaction (PCR) using solid-phase technology to obtain a large number of DNA sequences.

Synthesis and Expansion of DNA-encoded Glycan Allosteric Inhibitor Library

We use biological coupling techniques or chemical means to combine the DNA sequences obtained with compound molecules that have inhibitory effects on glycan modification, thus labeling each of the different compounds. Each of these DNA sequences is unique and corresponds to a specific compound molecule. In other words, each molecule has a unique DNA sequence attached to it, which is used for subsequent identification and tracking. All the DNA-compound hybrids obtained above are mixed to form a certain number of sub-libraries. Under similar conditions, all the prepared sub-libraries are mixed and the corresponding modified coding oligonucleotide chains are introduced by enzyme linkage. This cycle is repeated several times (usually 2-4 times) to obtain a DNA-encoded glycan allosteric inhibitor library containing a large number of compound molecules.

It is worth mentioning that the scale of the library we construct can be determined by the client's project needs.

Characterization

We analyze the library construction results in combination with mass spectrometry and other techniques.

In addition, to accelerate clients' discovery of glycan allosteric inhibitors, we not only provide personalization of the DNA-encoded glycan allosteric inhibitor library but also offer different packaging methods (e.g. powder packaging, solution packaging, etc.) for all the products in this library. This service will greatly enhance the efficiency and accuracy of glycan allosteric inhibitor screening and help clients find innovative candidate molecules faster.

Workflow

Utilizing advanced DEGL technology and extensive experience, our team of experts dedicated to DEGL research offers custom DNA-encoded glycan allosteric inhibitor libraries to accelerate drug discovery and biological research. Below is a workflow diagram of our services, detailing every step from initial design to final screening and validation, ensuring that our clients receive high-quality and efficient service support.

Applications

• The DNA-encoded glycan allosteric inhibitor library enables researchers to discover new glycan allosteric inhibitors, optimize the properties of drug molecules, and improve their efficacy, thereby accelerating the process of drug development.
• By screening and researching specific glycan allosteric inhibitors through the DNA-encoded glycan allosteric inhibitor library, scientists can gain a deeper understanding of the function of glycan molecules and their metabolic pathways in living organisms, and in turn, shed light on their mechanisms of action in diseases.
• The DNA-encoded glycan allosteric inhibitor library enables the screening of glycan allosteric inhibitors that can be used as biomarkers for early disease monitoring research.

Advantages

• Our DEGL research team is well versed in DNA encoding technology and multiple chemical synthesis routes and efficiently performs customized construction of DNA-encoded glycan allosteric inhibitor libraries.
• Our high-throughput screening platform is capable of rapidly screening millions of DNA-encoded glycan allosteric inhibitors, significantly shortening the development cycle.
• Our services are not only limited to the construction and screening of DNA-encoded glycan allosteric inhibitor libraries but also include subsequent analysis of results, structure optimization, and mechanism research.

Publication Data

Frequently Asked Questions

• What are glycan allosteric inhibitors?
  • Glycan allosteric inhibitors are a class of molecules that modulate the activity of a target, usually a protein or enzyme, by binding to an inactive site (a metastable site) and changing the conformation (i.e., three-dimensional structure) of the target. Instead of binding directly to the active site, these inhibitors indirectly inhibit the function of the target through a metastructural effect, unlike conventional inhibitors. In the case of glycan-modifying inhibitors, these molecules usually contain glycans or glycan derivatives, which are used to improve specificity and binding affinity.
• Which protein targets are suitable for modulation with glycan allosteric inhibitors?
  • Suitable targets for modulation with glycan allosteric inhibitors include, but are not limited to, enzymes, receptor proteins, and key regulatory proteins in signaling molecular pathways, which often have targets that can be modulated by alterations.

CD BioGlyco provides personalized DNA-encoded glycan allosteric inhibitor library construction services to clients in the long term with a deep DEGL technology background. We are committed to establishing long-term relationships with our clients and helping them take the lead in the highly competitive drug development process by providing comprehensive and efficient services. Please feel free to contact us if you have any needs or questions to explore the unlimited possibilities of glycan allosteric inhibitors.

• DNA-encoded Single-Pharmacophore Glycan Library
• DNA-encoded Dual-Pharmacophore Glycan Library
• DNA-encoded Trio-Pharmacophore Glycan Library
• Solid Phase-based DNA-encoded Glycan Library (DEGL)
• Liquid Phase-based DNA-encoded Glycan Library (DEGL)
• DNA-encoded Natural Glycan Library
• DNA-encoded Modified Glycan Library
• DNA-encoded Glycan Antigen Library
• DNA-encoded Glycopeptide Library
• Monovalent DNA-encoded Glycan Library (DEGL)
• Multivalent DNA-encoded Glycan Library (DEGL)
• Scaffold-based DNA-encoded Glycan Library (DEGL)
• Target-based DNA-encoded Glycan Library (DEGL)
• DNA-encoded Glycan Macrocycle Library
• DNA-encoded Glycan Covalent Inhibitor Library
• DNA-encoded Glycoprotein Degrader Library
• Light Crosslinking DNA-encoded Glycan Library (DEGL)
• DNA-encoded Dynamic library (DEDL)
• Coupling Reaction-based DNA-encoded Glycan Library (DEGL)
• Multi-component Reaction-based DNA-encoded Glycan Library (DEGL)
• Photocatalytic Cycloaddition-based DNA-encoded Glycan Library (DEGL)
• Aromatic Heterocyclization-based DNA-encoded Glycan Library (DEGL)
• Non-aromatic Heterocyclization-based DNA-encoded Glycan Library (DEGL)
• Carbocyclization-based DNA-encoded Glycan Library (DEGL)
• Macrocyclization-based DNA-encoded Glycan Library (DEGL)
• Click Chemistry-based DNA-encoded Glycan Library (DEGL)