Overview of DNA-encoded Glycan Library (DEGL)

CD BioGlyco has learned glycobiology experts to help clients plan complete DEGL solutions from library Design, construction, Screening, and Validation. In addition, CD BioGlyco offers customized glycan libraries to meet different clients' needs in specific research areas. Our researchers utilize DNA coding technology to link glycan chains to specific DNA sequences, enabling efficient screening and identification of a large number of glycan structures. This approach improves the throughput of glycan chain screening, making glycobiology research more efficient and precise.

Promoting DEGL with Confidence

DEGL consists of chimeric compounds formed by covalent coupling of glycans to single- or double-stranded DNA, and DEGL construction is the first step in DEGL application. Our experienced researchers provide comprehensive DEGL construction services.

By Encoding Strategy

CD BioGlyco often uses DNA-recorded synthesis, DNA template method, encoded self-assembling chemical (ESAC), and yoctoReactor-based DNA ligation method to construct DNA-encoded pharmacophore glycan library. We mainly provide DNA-encoded Single-Pharmacophore Glycan Library, DNA-encoded Dual-Pharmacophore Glycan Library, and DNA-encoded Trio-Pharmacophore Glycan Library.

By Synthetic Phase

Relying on robust synthesis technologies, we offer trusted Solid Phase-based DEGLs and Liquid Phase-based DEGLs. Our researchers use optimized solid-phase carriers or liquid-phase flow-through systems to construct specific DEGLs. For the isolated products, we use a range of advanced tools for purification and characterization. Our common tools include high-performance liquid chromatography (HPLC), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), and nuclear magnetic resonance (NMR).

By Library Type

CD BioGlyco offers many types of glycan libraries to meet the needs of different research areas. Our researchers have accumulated many years of experience in the design and construction of glycan libraries. The types of libraries we offer include but are not limited to:

By Chemical Reaction Type

Based on different types of chemical reactions, we offer comprehensive DEGL construction services. Our researchers efficiently synthesize structurally complex glycan molecules through precisely controlled coupling reactions and achieve precise one-to-one attachment with DNA tags. Utilizing the efficiency and versatility of multi-component reactions, we introduce multiple glycosyl units simultaneously in a one-pot reaction to rapidly construct large glycan libraries. Click chemistry plays an important role in DEGL construction due to its high efficiency, high selectivity, and ease of manipulation. Through the click reaction, the glycosyl units and DNA tags can be connected quickly and accurately to realize the efficient synthesis and characterization of glycan libraries. Enriching the tool library of glycobiology through a series of reactions also provides diverse resources for drug discovery and biomaterial development.

Workflow

Applications

• Drug discovery: DEGL can be used to screen glycan molecules with specific biological activities for the development of new drugs or vaccines.
• Biomarker studies: DEGL can be used to identify and characterize glycan structures associated with disease as biomarkers of disease.
• Biocatalysis: DEGL can be used to screen enzymes with specific catalytic activities, which in turn can be applied to biosynthetic and biotransformation processes.

Advantages of Us

• High-throughput screening: CD BioGlyco utilizes DNA coding technology to screen thousands of sugar chain structures simultaneously, greatly increasing efficiency.
• Highly customizable: Based on the client's needs, our team of trained experts provides personalized glycan library design and screening solutions.
• In contrast to traditional screening methods, we offer DEGLs that simultaneously screen multiple proteins or multiple conditions for the same target protein.

Publication Data

Frequently Asked Questions

• What are the main operational processes involved in DEGL?
  • The main processes of DEGL include library design, library synthesis, target affinity screening, decoding and structural analysis of active molecules, and validation of biological activity by re-synthesis of non-coding compounds.
• What are the synthetic advantages of the DNA ligation method?
  • DNA ligation methods do not require a pool of DNA templates, do not involve mismatches, and are relatively simple to design DNA sequences.

CD BioGlyco is committed to being your first choice in DEGL. We are constantly optimizing our glycan libraries and expanding our range of glycan species and reaction types. If you have any questions about glycan libraries, please feel free to contact us.

• DNA-encoded Glycan Library (DEGL) Design
• DNA-Compatible Reaction Development
• High-throughput Screening (HTS) of DNA-encoded Glycan Library (DEGL)
• Next-generation Sequencing of the High-throughput Screening (HTS) Output
• Data Analysis and Visualization
• Hit Validation and Assessment