DNA-encoded Trio-Pharmacophore Glycan Library
Overview of DNA-encoded Trio-Pharmacophore Glycan Library
With the continuous development of glycobiology, DNA-encoded glycan library (DEGL) is becoming more and more fleshed out as a novel library of combinatorial compounds. It can be a collection of simple small molecule compounds or a complex small molecule library with advanced spatial structure. CD BioGlyco provides clients with one-stop DEGL solutions from Glycan Library Design, Construction, and Screening to Validation. We strive to be your first choice for DNA-encoded pharmacophore glycan libraries such as Single-Pharmacophore Glycan Library, Dual-Pharmacophore Glycan Library, and trio-pharmacophore glycan library.
DNA-encoded Trio-Pharmacophore Glycan Library With Infinite Possibilities
Based on three pre-constructed sub-libraries (SL-A, B, and C), our researchers provide trio-pharmacophore glycan library design and synthesis services to clients. Our experienced library specialists help you solve all your library development problems.
Library Design and Synthesis
We synthesize SL-B using DNA templates with different reaction mechanisms. Our researchers provide 33-nt oligonucleotide design services. Moreover, we couple various bi-functional building blocks to 3' or 5' oligonucleotides to obtain 3' and 5' conjugates.
Based on different DNA-template reactions, we provide a wide range of DNA-compound-DNA conjugates preparation services. Moreover, we provide denaturing urea polyacrylamide gel electrophoresis (PAGE) assay, gel purification, molecular weight determination, and liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI/MS) analysis services.
Relying on a variety of chemical reaction reactions, our researchers provide reliable fragment sub-libraries (SL-A, C) preparation services. Our researchers use natural DNA PAGE to test the stability of three sub-library assemblies trio-pharmacophore glycan library.
Workflow
Based on our professional technical team, we provide DNA-encoded trio-pharmacophore glycan library design, build, and screen services. Our researchers place great emphasis on DNA-encoded trio-pharmacophore glycan library size and purity. This is our workflow.
Applications
- DNA-encoded trio-pharmacophore glycan library plays a key role in discovering small molecular binders against protein targets.
- DNA-encoded trio-pharmacophore glycan library is an effective tool for recognizing low-binding ligands.
- DNA-encoded trio-pharmacophore glycan library can be used for de novo identification of fragments.
Advantages of Us
- The DNA-encoded trio-pharmacophore glycan library we provide selects fragments and linkers simultaneously.
- Despite the assembly of three fragments, the resulting compounds met the latest molecular weight criteria.
- We offer SL-B with versatility which can be used both as a binding fragment and as a linker fragment.
Publication Data
Technology: Denaturing TBE-urea polyacrylamide gel electrophoresis (PAGE), Ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), Trypsin inhibition assay, Quantitative real-time PCR (qPCR), Carbonic anhydrase II inhibition assay
Journal: Nature Communications
Published: 2023
IF: 16.6
Results: In this study, based on assembling three pre-purified and validated sub-libraries, researchers constructed a trio-pharmacophore DNA-encoded chemical library for simultaneous screening of small molecule fragments and linkers. The researchers ligated three different fragments together, encoded by DNA tags, and displayed them. Screening and validation successfully identified several novel small molecule compounds with high binding capacity. The technique identified and optimized several optimizable drug precursors in a short period, bringing innovative tools to the field of drug discovery.
Fig.1 Schematic representation of trio-pharmacophore DNA-encoded library. (Cui, et al., 2023)
Frequently Asked Questions
- What are the chemical reactions involved in library synthesis?
- Library synthesis using various reaction types such as reductive amination, amide bond generation, Michael addition, azide-alkyne cycloaddition, and Diels-Alder reaction.
- How to ensure the high purity of sublibraries?
- Each DNA-compound-DNA conjugate is encoded by splint ligation. Our researchers use PAGE and HPLC to purify the conjugates and characterize them by MS to ensure the high purity of the library members.
Rely on a professional team, CD BioGlyco satisfactory DNA-encoded trio-pharmacophore glycan library construction programs. According to the client's needs, our lab provides personalized glycan library construction solutions. For any further information, do not hesitate to contact us.
Reference
- Cui, M.; et al. Trio-pharmacophore DNA-encoded chemical library for simultaneous selection of fragments and linkers. Nature Communications. 2023, 14(1): 1481.
For research use only. Not intended for any clinical use.
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