DNA-encoded Glycan Antigen Library
Overview of DNA-encoded Glycan Antigen Library
At CD BioGlyco, we are focused on promoting the development of next-generation functional glycomics and providing powerful tools for life science research by building a high-precision and high-efficiency DNA-encoded Glycan Library. DNA-encoded glycan antigen library uses the unique sequence of DNA as the "barcode" of glycan antigens. Through PCR amplification and DNA sequencing technology, the library can achieve efficient screening and quantitative analysis of complex glycan antigens. This technology not only overcomes the challenges of low sensitivity and large sample requirements in traditional glycomics research but also greatly improves the accuracy and throughput of research.
Revolutionize Glycan Research: Unveil Hidden Patterns with DNA-encoded Glycan Antigen Libraries
We use advanced computer algorithms to convert the complex structural information of glycan antigens into unique DNA barcodes. This strategy ensures that each glycan antigen can be uniquely identified, thereby improving the diversity and specificity of the library.
DNA-Glycan Conjugation Strategy
- Click chemistry: We use efficient Click Chemistry reactions such as copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction to achieve efficient and stable connection between DNA and glycan antigens. This reaction has the advantages of mild conditions, high yield, and few by-products, which is very suitable for the construction of large-scale DNA-encoded glycan antigen libraries.
- Product structure: After the reaction, we obtain conjugates of glycan antigens and DNA strands. These conjugates not only retain the biological activity of glycan antigens but also possess characteristics of amplification and sequencing.
Library Construction
- Glycan antigen synthesis: First, we design and synthesize glycan antigens with specific structures according to client needs.
- DNA barcode design: We use computer algorithms to design unique DNA barcodes for each glycan antigen.
- DNA-Glycan coupling: We covalently bind DNA barcodes to glycan antigens through click chemistry.
We link molecular components with corresponding DNA tags that are connected to the initial DNA molecule through specific chemical reactions to produce DNA-encoded glycan antigens. After the reaction is complete, these glycans attached to the DNA are combined and the 1-cycle library is purified and re-dissolved. It is then divided equally and undergoes the same process for the chosen on-DNA conjugation reaction to construct a 2-cycle DNA-encoded glycan antigen library.
We have strong customized synthesis capabilities and build compound libraries ranging from millions to tens of millions according to client needs. We use advanced automated synthesis platforms and efficient chemical reactions to ensure that each glycan antigen molecule can be accurately linked to the corresponding DNA barcode.
Workflow
Demand Analysis: Determine the type, quantity, and complexity of the required glycan antigens according to client needs.
Glycan Antigen Synthesis: Prepare glycan antigen precursors with azide groups through chemical synthesis methods.
Click Chemical Conjugation: Use CuAAC reaction to covalently link glycan antigens to DNA to form glycan antigen-DNA conjugates, thus constructing a DNA-encoded glycan antigen library.
Quantitative Polymerase Chain Reaction (qPCR) Detection: Use qPCR technology to perform high-sensitivity detection of glycan antigen-DNA conjugates to achieve quantitative analysis of glycan antigens.
Data Analysis and Reporting: Perform bioinformatics analysis on sequencing results, parse glycan antigen information, and provide clients with detailed research reports.
Applications of DNA-encoded Glycan Antigen Library
- Biomarker screening and discovery: DNA-encoded glycan antigen libraries can be used to screen and identify potential biomarkers. Glycan structures associated with specific diseases or physiological states can be identified as potential biomarkers by constructing a library containing a variety of glycan antigens and reacting with antibodies in various biological samples such as blood, tissue fluid, etc.
- Glycosylation proteomics research: DNA-encoded glycan antigen libraries can be used to explore the interaction between glycans and proteins. The functions and regulatory mechanisms of these proteins can be studied by mixing the glycan antigens in DNA-encoded glycan antigen libraries with protein samples and using affinity capture technology to screen out proteins that bind to specific glycans.
- Target identification in drug development: DNA-encoded glycan antigen libraries can be used to identify glycan structures that interact with drug targets. Glycans that specifically bind to drugs can be screened by constructing a library containing glycans related to potential drug targets and reacting with drug candidates, thereby revealing the drug mechanism of action and potential targets.
Advantages
- Customized synthesis: We provide customized synthesis services and build a compound library ranging from millions to tens of millions according to client needs.
- Efficient coding system: We use advanced computer algorithms to accurately analyze the potential activity of complex and diverse glycan structures.
Publication
Technology: A process for assigning unique codes to all glycans by utilizing a software tool that transforms their structural information into DNA barcodes.
Published: 2017
Results: The author proposed a method for uniquely coding all glycans using a computer program designed to convert the structural information of glycans to DNA barcodes. This unique and universal coding system for glycans aimed to benefit both the synthesis and analysis of DNA-encoded glycan libraries (DEGL). As a proof-of-concept study, the author demonstrated a small DEGL of blood and globo series glycan antigens, which was applied in detecting blood groups and breast cancer from plasma.
Fig.1 Flowchart describing decoding. (Parameswaran, 2017)
Frequently Asked Questions
- What is a DNA-encoded glycan antigen library and what are its applications in biotechnology?
- DNA-encoded glycan antigen library is an innovative biotechnology tool that enables high-sensitivity detection and analysis of glycan antigens by assigning a unique DNA barcode to each glycan antigen. DNA-encoded glycan antigen library is of great significance in functional glycomics research, helping scientists understand the role of glycans in biological processes such as intercellular communication, pathogen recognition, immune response, and disease occurrence. In addition, DNA-encoded glycan antigen library can also be used in the discovery of disease markers, drug screening, and personalized medicine.
- What are the advantages of DNA-encoded glycan antigen library compared with traditional glycan detection methods?
- DNA-encoded glycan antigen library has the following significant advantages compared with traditional glycan detection methods:
- High throughput: Next-generation sequencing applications enable DNA-encoded glycan antigen libraries to process thousands of glycosylated antigens simultaneously, greatly improving research efficiency.
- Reduced sample requirements: Due to the sensitivity of PCR, the amount of sample required for DNA-encoded glycan antigen library is greatly reduced, which is especially important for precious or difficult-to-obtain samples.
- The reliability of screening results can be improved by DNA coding.
CD BioGlyco has advanced chemical synthesis technology and a professional technical team, that can efficiently and accurately synthesize various complex structure glycan antigens. Our synthesis capabilities cover a wide range from simple monosaccharides to complex polysaccharides, meeting the needs of different clients for the number and complexity of compound libraries. We provide high-quality customized services whether it is a library of millions or tens of millions. Please feel free to contact us for more details if you are interested in our DNA-encoded glycan antigen library construction service!
Reference
- Parameswaran, A. DNA encoded libraries (DEGL) of glycan antigens to detect antibodies: an approach towards next generation functional glycomics. Thesis. Georgia State University. 2017.
For research use only. Not intended for any clinical use.
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