Overview of DNA-encoded glycan library (DEGL) Screening for Antibody Target Identification

Glycans and glycoconjugates play very important roles in various biological processes such as cell-cell interactions, immune processes, stabilization of proteins, cancer, and other diseases, etc. The development of various types of antibodies using them as therapeutic targets is essential for the development of effective treatments. DEGL is an innovative strategy with important applications in screening antibody-binding ligands in a high-throughput manner. With extensive hands-on experience in identifying antibody targets, CD BioGlyco offers reliable and customized DEGL screening solutions. The diversity and high quality of our DEGLs ensure the highest level of success.

Highest Hit Rate Through Diverse DEGLs

We have extensive research experience in antibody biology and have developed a variety of screening strategies. DEGL screening for antibody target identification includes custom synthesis of DEGLs, affinity screening of target antibodies, decoding and structural analysis of glycans, re-synthesis of non-coding glycans, and validation of binding properties of glycans to target antibodies. The specific screening process is described below:

Library Design and Construction

We chemically synthesize a variety of glycan structures, such as the global-sugar series, according to antibody type and binding characteristics, and achieve DNA-Glycan coupling through click chemistry. Our team custom synthesizes libraries of various orders of magnitude according to client needs to improve hit rates.

Library Screening

DEGL is incubated with a labeled antibody under specific conditions to allow binding interactions to occur between the glycan and the antibody. After incubation, an affinity screen is performed to isolate glycan-DNA affixes that have bound to the antibody and to elute unbound molecules.

Next-generation Sequencing (NGS) and Data Analysis

DEGL screening is followed by decoding and data analysis. We use polymerase chain reaction (PCR) to amplify the DNA tag and then sequence it to identify the specific glycan structure that binds to the antibody. We use advanced algorithms and bioinformatics tools for efficient processing and high-throughput analysis of screening data.

Hit Validation and Evaluation

After decoding the small molecules that bind to the target antibody, we further screen the well-characterized molecules for de-DNA resynthesis by affinity mass spectrometry. Afterward, their binding affinity and biological activity with the antibody target are tested and further characterized to understand the binding mechanism and provide certain optimization insights for subsequent studies.

Workflow

We provide one-stop experimental services and customized solutions using a diversity of DEGLs to screen antibody-targeted ligands.

Applications

• DEGL screening to identify antibody-targeting ligands for optimizing the binding affinity, specificity, or other desired characteristics of an antibody.
• DEGL screening helps identify novel glycan targets for antibodies, providing insights into new therapeutic opportunities and potential targets for drug development.
• DEGL screening to identify antibody-targeting ligands can help elucidate pathways involved in disease processes or understand the mechanism of action of therapeutic antibodies.

Advantages

• We offer a full range of DEGL screening solutions, including antibody production, DEGL construction, screening, data analysis to validation of hit compounds.
• We tailor a variety of DEGLs to maximize hit rates based on our client's research needs.
• We construct DEGLs that allow rapid screening of a large number of glycan structures, resulting in the identification of antibody-specific glycan ligands with high specificity and affinity.

Publication Data

Frequently Asked Questions

• What are the advantages of DEGL screening in antibody target identification compared to other means?
  • The main techniques used to study glycoprotein interactions and identify antibody-targeted glycans are surface plasmon resonance, enzyme-linked lectin analysis, glycan microarrays, etc. DEGL screening has been validated in the identification of antibody-targeted ligands and serves as a potential alternative or complement to glycan detection techniques. The technique constructs various DEGLs and incubates them with specific antibodies for sensitive detection and identification by PCR and DNA sequencing, and ultimately screens for antibody-targeted glycans. DEGL screening not only allows for simultaneous screening of multiple or the same target antibody for multiple conditions, but also offers tremendous advantages in terms of difficulty in building compound libraries in terms of size, screening efficiency, and expense.
• Does DEGL screening have any special requirements for antibodies?
  • DEGL Screening is available for a wide range of antibodies. We design customized screening protocols based on the study requirements, incubating various orders of magnitude glycan libraries with the target antibody to isolate the glycan structures that bind to the antibody. DNA tags are then sequenced to identify specific glycan structures that interact with the antibody.

CD BioGlyco provides valuable insights into antibody targets and potential therapeutics opportunities in drug discovery and development through rapid, high-throughput identification of glycan structures interacting with antibodies by DEGL screening. Welcome to contact us for customized screening solutions for DEGL and the identification of antibody targets.

• Magnetic Bead-based DEGL Affinity Screening
• Resin Tip-based DEGL Affinity Screening
• Interaction-dependent PCR (IDPCR)-based DEGL Screening
• DNA Photoaffinity Labeling-based DEGL Screening
• Microfluidic Processor-based DEGL Screening
• Cell-based DEGL Screening
• DNA Programmed Affinity Labelling (DPAL)-based DEGL Screening
• DEGL Screening for Glycan-Protein Interaction Study
• DEGL Screening for Protein-Protein Interaction Inhibitor Discovery
• DEGL Screening for G Protein-coupled Receptor-Binding Compounds Discovery
• DEGL Screening for Protein Ubiquitination Study
• DEGL Screening for Bacterial Enzyme Study
• DEGL Screening for Protein-templated Ligand Discovery