DNA-encoded Natural Glycan Library
Overview of DNA-encoded Natural Glycan Library
Efficient DNA coding technologies are gradually leading the way in glycan science, providing powerful tools for glycan-related drug discovery and disease mechanism research. CD BioGlyco provides clients with custom glycan library construction and High-throughput Screening services to meet clients' needs at different stages of research. Our lab offers a wide range of DNA-encoded Glycan Library (DEGL) such as natural glycan library, Modified Glycan Library, Glycan Antigen Library, and Glycopeptide Library.
Build DNA-encoded Natural Glycan Library with Us Beyond Your Imagination
Our specialized library construction team uses a headpiece conjugation-code ligation (HCCL) strategy to combine glycans with unique DNA coding, enabling the construction of natural glycan libraries with high diversity and affinity. This strategy significantly improves screening efficiency and shortens development cycles. Our advanced HCCL strategy and high-throughput screening services ensure rapid screening of physiologically active glycan molecules from large libraries.
Unique DNA Coding of Glycans
Relying on the glycan DNA coding system, we attach unique DNA codes to monovalent or multivalent headpieces. This modular strategy contains single or multiple copies of DNA-encoded glycans, respectively. Moreover, our dedicated team provides programmed glycan coding sequences designed so that each member of the library has a unique identifier that can be traced. Moreover, the glycans we provide display a wide range of glycan residue and non-glycan residue combinations, glycosidic bonding bonds, stereochemistry, and positions.
Conjugation and Display Strategy for DNA-encoded Glycans
With the help of click-chemistry, and amino-active cross-linking, we offer direct coupling and reductive amination of DNA. This strategy significantly improves the synthesis efficiency, especially in terms of precise control at the sub-nanometer level. Our researchers used an altered version of split and pool synthesis, the HCCL approach to generate DNA-glycans.
DNA-encoded Natural Glycan Library Generation
Utilizing advanced techniques, we provide a DNA-encoded natural glycan library with higher content and throughput. Importantly, unique coding regions correspond to the identity of each glycan.
Workflow
Based on the client's needs, we offer the construction of specific natural glycan libraries. Glycan libraries are customized by our team of experts to meet the desired specifications of the client. This includes selecting the appropriate glycan and designing the unique DNA code to be ligated to it. The process of constructing DNA-encoded natural glycan libraries usually includes the following steps: design of coding strategies, synthesis of DNA fragments with different glycan structures, library construction and optimization, validation, library storage, and database management.
Applications
- DNA-encoded natural glycan library can be used for specific lectin ligand screening.
- DNA-encoded natural glycan libraries play an important role in glycan binding analysis and increase access to low-cost glycan analysis.
- DNA-encoded natural glycan library is becoming increasingly important in glycan-related drug screening.
- DNA-encoded natural glycan library can be used for next-generation glycan microarrays.
Advantages of Us
- CD BioGlyco offers a natural glycan library that is not only highly specific but also contains a diverse range of glycan structures.
- Our researchers control the number of glycans presented on each headpiece and the spatial display of glycans by multivalent headpiece.
- We provide our clients with natural glycan libraries from different sources with highly specialized.
Publication Data
Technology: Enzymatic synthesis, Primer extension (PEX), Polymerase chain reaction (PCR), Fluorescent scan
Journal: Chemistry-A European Journal
Published: 2024
IF: 3.9
Results: In this work, researchers introduced a variety of monosaccharides into the DNA molecule using base modification techniques and hypermodification. These modifications were mainly focused on the bases of DNA, and an efficient enzymatic synthesis reaction was successfully achieved by modifying DNA polymerase and synthesizing nucleotide substrates with sugar molecules. The researchers further explored the potential impact of different sugar units on the overall stability and functionality of DNA. This study provides ideas for further exploration of glycosylated DNA libraries in the future.
Fig.1 Schematic of modified DNA by enzymatic synthesis. (Krömer, et al., 2024)
Frequently Asked Questions
- What is HCCL?
- HCCL is a two-step modular strategy for generating glycan conjugates. Our researchers directly coupled the DNA headpiece to glycan as the first step. In the second step, we attach the desired DNA to the glycan via the headpiece.
- What are the advantages of HCCL?
- HCCL eliminates the need to customize specific connectors for each unique DNA sequence. Only chemical modification of the headpiece is required, which allows HCCL to be mass-produced and suitable for a wide range of different glycans in DEGL. Moreover, HCCL is capable of being modified by any unique DNA coding sequence.
CD BioGlyco is committed to advancing the field of glycobiology by providing efficient natural glycan library construction solutions and cutting-edge technology. Our scientists are also working on improving the efficiency of conjugation processes, which further enhance the quality and utility of our libraries. Additionally, our team is always ready to offer personalized natural glycan libraries and guidance to ensure that our clients' projects are successful. Please feel free to contact us.
Reference
- Krömer, M.; et al. Glyco-DNA: Enzymatic synthesis of base-modified and hypermodified DNA displaying up to four different monosaccharide units in the major groove. Chemistry-A European Journal. 2024: e202402318.
For research use only. Not intended for any clinical use.
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