Microfluidic Processor-based DEGL Screening
Overview of Microfluidic Processor-based DEGL Screening
Microfluidic processor-based DNA-encoded glycan library (DEGL) screening combines microfluidics with DNA-encoded libraries to enable high-throughput and precise functional screening of glycans. This technology leverages the ability of microfluidic circuits to handle tiny volumes and integrate multiple steps in a continuous flow, which is ideal for screening complex libraries like DEGL. The microfluidic system automates the entire screening process, from the encapsulation of glycan-encoded beads in droplets to the photochemical release of glycans, incubation, and detection of interactions based on fluorescence.
Maximize Precision, Accelerate Discovery with CD BioGlyco's Microfluidic DEGL Screening
CD BioGlyco's microfluidic processor-based DEGL screening is designed to push the boundaries of glycan-based research and drug discovery. By integrating cutting-edge microfluidic technology with DNA-encoded libraries, this method ensures precise control over the screening environment, enhances throughput, and reduces sample usage. This streamlined approach allows researchers to conduct high-throughput screenings with unparalleled efficiency, making it an exceptionally powerful tool for identifying glycan interactions and accelerating the discovery of new therapeutics. Whether you are exploring complex glycan interactions or seeking novel drug candidates, our DEGL screening offers the precision and scalability required to achieve breakthrough results.
Workflow
Library preparation
A DNA-encoded glycan library is prepared, where each glycan is linked to a unique DNA sequence that serves as its identifier.
Bead encapsulation
The glycan-encoded beads are suspended and introduced into the microfluidic circuit, where they are encapsulated into picoliter droplets containing assay reagents.
Photochemical cleavage
Inside the microfluidic device, the droplets pass through a UV irradiation zone, where the glycans are photochemically cleaved from their beads. This step releases the glycans into the solution, making them available to interact with the target molecules within the droplets.
Incubation
The droplets are then routed through an incubation channel, allowing sufficient time for any interactions between the glycans and the target molecules to occur. The microfluidic environment ensures uniform incubation conditions across all droplets.
Fluorescence detection
After incubation, each droplet's fluorescence intensity is measured using a laser-induced fluorescence (LIF) detector. The intensity indicates the presence of glycan-target interactions, with lower fluorescence suggesting successful inhibition or binding activity.
Droplet sorting
Based on the fluorescence intensity, the microfluidic system sorts the droplets. Droplets with significant glycan-target interactions are deflected into a collection channel for further analysis, while non-interacting droplets are discarded.
Data analysis
The DNA sequences from the sorted droplets are sequenced to identify the glycans responsible for the observed interactions. This sequencing data is then analyzed to determine the specificity and potential efficacy of the glycans in binding or inhibiting the target.
Validation
The identified glycan-target interactions are further validated through additional assays or functional studies to confirm their biological relevance and potential as therapeutic candidates.
Applications
- The microfluidic processor allows for the high-throughput screening of vast DEGL, enabling researchers to identify potent glycan interactions with disease-relevant targets, such as proteins or receptors.
- Our service can be used to identify novel biomarkers associated with specific glycan interactions.
- Our service can screen large glycan libraries efficiently allowing for the identification of glycans that interact with immune system targets.
Advantages
- The microfluidic processor automates the screening of large glycan libraries, significantly increasing throughput compared to traditional methods.
- The microfluidic system operates at picoliter scales, minimizing the amount of reagents and samples required for each assay.
- The microfluidic processor integrates multiple steps of the screening process into a single, continuous workflow.
- The platform is highly scalable, making it suitable for both small-scale academic research and large-scale industrial drug discovery projects.
Publication
Technology: DNA-encoded synthesis, Combinatorial compound libraries, Miniaturized automation
Journal: ACS Combinatorial Science
IF: 3.9
Published: 2017
Results: The study demonstrated the effectiveness of integrating a microfluidic processor with DNA-encoded combinatorial libraries for functional screening. The microfluidic circuit successfully automated the process of distributing DNA-encoded compound beads into picoliter-scale droplets, photochemically cleaving compounds from the beads, incubating the assay, and detecting and sorting droplets based on fluorescence intensity. This approach allowed for high-throughput screening of compound libraries, leading to the identification of specific inhibitors with a low false discovery rate. The successful implementation of this technology shows its potential for efficient and scalable small-molecule discovery.
Fig.1 Microfluidic processor for DNA-encoded combinatorial library functional screening. (MacConnell, et al., 2017)
Frequently Asked Questions
- What are the benefits of using a DEGL?
- DEGL allows for the creation of vast libraries with each glycan linked to a unique DNA barcode, enabling high-throughput identification through sequencing. This approach facilitates efficient screening and precise identification of glycan interactions with potential therapeutic or diagnostic applications.
- Is the service scalable for large projects?
- Yes, the service is highly scalable and can be adapted for both small-scale academic research and large-scale industrial drug discovery projects. The microfluidic platform is flexible and can accommodate varying library sizes and screening volumes.
At CD BioGlyco, our microfluidic processor-based DEGL screening service represents the pinnacle of efficiency and precision in glycan interaction research. By combining state-of-the-art microfluidic technology with DEGL, our service enables high-throughput screening with exceptional control over assay conditions. Please feel free to contact us for more information to discover how we can support your research.
Reference
- MacConnell, A.B.; et al. An integrated microfluidic processor for DNA-encoded combinatorial library functional screening. ACS Combinatorial Science. 2017, 19(3): 181-192.
For research use only. Not intended for any clinical use.
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