Multi-component Reaction-based DNA-encoded Glycan Library (DEGL)
Overview of Multi-component Reaction-based DEGL
Multi-component reaction (MCR) is a class of highly efficient chemical reactions capable of generating complex products through the interaction of multiple reactants in a single stage. Compared to traditional stepwise synthesis methods, MCRs offer significant advantages, such as simplified reaction steps, high atom economy, low by-product generation, and rapid generation of diverse compound libraries. In the fields of drug discovery and chemical biology, MCRs are increasingly used for the construction of DNA-encoded Glycan Libraries (DEGLs) due to their rapid and efficient properties.
At CD BioGlyco, we have a dedicated research team that provides professional DEGL construction services to researchers in various industries. Here, we utilize advanced technologies such as MCRs to construct high-quality and diverse DEGLs for our clients.
Unleash Innovation: Explore New Horizons with DEGL
We utilize advanced MCR technology to efficiently synthesize DEGL. Our services cover the entire process from designing reactions and optimizing conditions to product purification and library construction, and we are committed to providing clients with high-quality and diverse glycan libraries to meet a wide range of research needs.
Selection of MCR Systems
At CD BioGlyco, we are particularly careful about the selection of MCR systems. Combined with the characteristics of the target libraries, the selection of efficient and stable MCR systems will not only improve the efficiency of DEGL synthesis but also ensure the diversity and quality of DEGL.
Here, we provide various types of MCR systems, such as the Ugi reaction, Passerini reaction, and so on. These reactions are not only able to occur under mild conditions but also allow flexible combinations of multiple components.
Construction of DEGL
Each glycan molecule corresponds to a unique DNA tag, which facilitates the identification and tracking of the molecules in the subsequent screening process. Also, the MCR conditions (e.g., reaction temperature, solvent, or pH) can be controlled to maximize the efficiency and yield of the reaction. In addition, the above process can be repeated by using the first round of synthesis products as reactants for a new round of MCR to further increase the diversity of glycan and DNA combinations. This library can be used for High-throughput Screening to discover biologically active glycan molecules.
Workflow
MCR-based DEGL construction technology provides breakthroughs in medical research, drug discovery, and chemical biology research. Our service covers the whole process from design, and synthesis to screening, which will help clients to discover functional molecules efficiently and promote innovative research. The following workflow diagram will show our service flow in detail.
Applications
- MCR-based DEGL can be used for high-throughput screening of a large number of specific glycan compounds, helping researchers discover molecules with therapeutic potential.
- MCR-based DEGL can be used to study the interactions between glycans and proteins, cell surface receptors, or pathogens, helping researchers to understand the mechanisms of molecular recognition and providing important information for biological and medical research.
- MCR-based DEGL can be used to generate and screen glycan molecules associated with specific diseases, leading to the development of new biomarker assays.
Advantages
- We provide custom design and synthesize DEGLs by selecting the appropriate MCR based on the specific needs of our clients.
- With the help of MCR, we combine multiple compound units in a single step to rapidly construct DEGLs of high complexity. Our high-throughput synthesis and parallel reaction strategy greatly improve the diversity and coverage of DEGLs.
- We not only provide DEGL construction services but also include efficient screening and analysis services. Using advanced high-throughput screening platforms and bioinformatics tools, we help clients rapidly identify and validate target molecules.
Publication Data
Technology: MCR and Ugi reaction
Journal: RSC Chemical Biology
IF: 4.2
Published: 2022
Results: MCR can lead to the generation of various compounds in a single-step reaction. Among them, the Ugi reaction can easily provide peptide-like libraries. In this article, focusing on the coupling of DNA to ligands in DEL synthesis, the authors have researched the reactions between oligonucleotides and peptidoglycans prepared via the Ugi reaction and the subsequent binding reactions of peptidoglycans labeled with substituted oligonucleotides. Specifically, the authors used 6-(4-nitrophenoxycarbonylamino) hexanoic acid and N-4-nitrophenoxycarbonylglycine as carboxylic acids to obtain peptidomimetic peptides with an activated carbamate portion as products of the Ugi reaction. These peptides were then treated with oligonucleotides bearing 5′- or 3′-terminal aminohexyl junctions to obtain various oligonucleotide-labeled peptide-like compounds. In addition, the method was found to be tolerant of many different functional groups.
Fig.1 Ligation of active carboxylic acid-derived Ugi reaction products to DNA. (Kita, et al., 2022)
Frequently Asked Questions
- What is DEGL?
- DEGL is an emerging tool that combines glycan chemistry and DNA coding technology to contain a large number of diverse glycan structures, each encoded and labeled by a unique DNA fragment.DEGL can be used to efficiently screen for glycan molecules interacting with the recognition of specific targets.
- What role do MCRs play in DEGL?
- DEGL is a powerful and sensitive method for large-scale screening and identification of small molecules interacting with target proteins. The application of MCRs in DEGL not only significantly enhances the diversity and complexity of libraries, but also reduces the cost and time of the synthesis process. Especially for glycans, their complex structures and diverse functional groups make efficient synthesis by MCR possible, which in turn accelerates the construction and screening of glycan libraries.
CD BioGlyco's DEGL research team is dedicated to the custom construction of many types of DEGLs. Through our efforts, clients can obtain high-affinity glycan candidates in a short time, further advancing the drug discovery and development process. Please feel free to contact us if you are interested in DEGL, our team provides detailed technical consultation and customized solutions.
Reference
- Kita, R.; et al. Conjugation of oligonucleotides with activated carbamate reagents prepared by the Ugi reaction for oligonucleotide library synthesis. RSC Chemical Biology. 2022, 3: 728-738.
For research use only. Not intended for any clinical use.
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