Overview of IDPCR-based DNA-encoded glycan library (DEGL) Screening

Interaction-dependent PCR (IDPCR) is a method designed to identify binding pairs from libraries of ligands and targets in a solution-phase experiment. IDPCR relies on the principle that the binding between a DNA-linked ligand and a DNA-linked target brings together complementary DNA sequences, allowing them to form a duplex. This duplex can then be extended by polymerase, resulting in a DNA strand that contains the sequence information for both the ligand and the target, which is subsequently amplified by PCR. This selective amplification enables the identification of specific ligand-target interactions with high sensitivity and specificity.

Amplify Glycan Discoveries with IDPCR-based DEGL Screening

At CD BioGlyco, our IDPCR-based DEGL screening service employs the innovative IDPCR method for high-throughput glycan screening. In this process, each glycan in the library is linked to a unique DNA sequence, forming the DEGL. When these glycans bind to a target of interest, IDPCR selectively amplifies the DNA sequences corresponding to the binding pairs. This amplification enables the rapid and efficient identification of glycan-binding proteins or other target molecules, which is crucial for the discovery of new glycan-based therapeutics and diagnostics. The service is designed to streamline the identification process, providing valuable insights into glycan interactions that can drive the development of novel medical and research applications.

Workflow

Applications

• Our service is instrumental in identifying glycan-binding proteins that could serve as novel drug targets, aiding in the development of glycan-based therapeutics for various diseases.
• The High-Throughput Screening capability allows for the identification of glycans that interact with disease-related proteins, facilitating the discovery of biomarkers for disease.
• By identifying glycans that interact with immune system targets, this service can support the development of glycan-based vaccines, particularly for pathogens that utilize glycan structures in infection mechanisms.
• Researchers can use our service to explore the role of glycan interactions in various biological processes.

Advantages

• The IDPCR method allows for the simultaneous screening of vast glycan libraries, accelerating the identification of relevant glycan-target interactions.
• Using DNA encoding ensures that only specific glycan-target interactions are amplified and detected, minimizing false positives and enhancing the accuracy of results.
• Our service can be applied to a wide range of targets, including proteins, cells, and small molecules, making it suitable for diverse research and development needs.

Publication

Frequently Asked Questions

• What types of targets can be screened using this service?
  • The service is versatile and can screen a wide range of targets, including proteins, cells, and small molecules. This flexibility makes it suitable for various applications in both academic research and industrial development.
• How can this service contribute to drug discovery?
  • By identifying specific glycan-binding proteins, the IDPCR-based DEGL screening service can uncover new therapeutic targets and guide the development of glycan-based drugs. This is particularly valuable for diseases where glycan interactions play a critical role, such as cancer or infectious diseases.

At CD BioGlyco, our DEGL screening service seeks to uncover complex glycan interactions with unparalleled precision. This service leverages cutting-edge IDPCR technology to rapidly and efficiently identify glycan-binding targets, facilitating breakthroughs in drug discovery, biomarker identification, and vaccine development. Please feel free to contact us to discover how we can support your glycan-related research.

• Magnetic Bead-based DEGL Affinity Screening
• Resin Tip-based DEGL Affinity Screening
• DNA Photoaffinity Labeling-based DEGL Screening
• Microfluidic Processor-based DEGL Screening
• Cell-based DEGL Screening
• DNA Programmed Affinity Labelling (DPAL)-based DEGL Screening
• DEGL Screening for Glycan-Protein Interaction Study
• DEGL Screening for Protein-Protein Interaction Inhibitor Discovery
• DEGL Screening for G Protein-coupled Receptor-Binding Compounds Discovery
• DEGL Screening for Protein Ubiquitination Study
• DEGL Screening for Bacterial Enzyme Study
• DEGL Screening for Antibody Target Identification
• DEGL Screening for Protein-templated Ligand Discovery