DNA-compatible Functional Group Transformation Development
Overview of the Development of DNA-compatible Chemistry
DNA-compatible chemistry is designed to optimize and advance chemical reaction processes so that they can be efficiently compatible with DNA-encoded library (DEL) systems. This technology combines the unique advantages of DNA encoding with an innovative approach to chemical reactions, opening up new possibilities for drug discovery, functional screening, and chemical research. At the heart of DNA-compatible chemistry development lies the design and optimization of chemical reactions that work seamlessly with DNA labeling systems. This compatibility ensures that the chemical properties of the compound and the stability of the DNA labeling during the reaction will not be affected by changes in reaction conditions that would affect the potency and stability of the compound. By optimizing the reaction conditions, the chemistry can be used to synthesize compounds efficiently while maintaining the stability of DNA labeling, thus expanding the chemical diversity of compound libraries.
Unlocking Precision: DNA-Compatible Reactions for Cutting-Edge Discoveries
CD BioGlyco has advanced technologies and platforms to provide our clients with specialized DNA-compatible Chemistry Development Services for DNA-encoded Glycan Library (DEGL) Construction. Our DNA-compatible chemistry development includes but is not limited to DNA-compatible functional group transformation development and DNA-compatible Bond Formation Development. The DNA-compatible functional group transformation development services we provide include but are not limited to the following.
Workflow
Scheme Design
We start by having a detailed discussion with the client to fully understand their specific requirements and objectives for functional group transformation. This includes identifying the necessary chemical reaction type and application area. Subsequently, we formulate a customized project plan based on the client's needs and develop and design functional group transformation reactions that are compatible with DNA labeling. We take into consideration both reported reaction conditions in the existing literature and those commonly used in traditional chemical synthesis.
Reaction Development and Condition Optimization
Our experts synthesize the target compounds in the laboratory according to the designed experimental conditions and perform quality testing of the transformed compounds with DNA-compatible functional groups. At the same time, we combine the optimization of the reaction conditions, including temperature, solvent, catalyst, etc., through experiments to improve the conversion efficiency and selectivity, while maintaining the stability of the compounds.
Applications
- DNA-compatible reaction development supports the optimization of DEGL, enabling the creation of a broader chemical space and enhancing the quality and diversity of compound libraries, thereby increasing the opportunity for the discovery of new molecules.
- In performing functional screening, DNA-compatible reactions ensure accurate screening and validation of functional compounds from the DEGL.
- DNA-compatible reaction development ensures that the compounds in libraries are compatible with DNA labeling during screening and identification, resulting in improved screening efficiency and accuracy.
Advantages
- DNA-compatible reactions are designed to take into account the interaction of the compound with DNA, ensuring that the compound remains stable and reduces degradation or deterioration when stored and processed in the library.
- DNA-compatible reactions allow compounds to maintain their biological activity during the screening process, thus ensuring the accuracy and validity of the screening results.
- DNA-compatible reactions are designed to ensure that compounds interact with DNA as expected, reducing the potential for non-specific reactions and increasing the reliability of experimental results.
Publication Data
Technologies: DEL
Journal: RSC advances
Published: 2021
IF: 3.9
Results: This article focuses on some of the chemical methods used in DELs, as well as the application and output of these methods in DELs. The article mentions several DNA-compatible reactions, including the Suzuki-Miyaura reaction, the Heck reaction, the C-H activation reaction, the Suzuki-Miyaura coupling reaction, and the ring-closing and ring-opening reactions. The optimized conditions and catalyst selection for these reactions make them suitable for DNA-encoded compounds, leading to efficient synthesis in DELs. The article also mentions some cases where these reactions have been applied to construct DELs and their outputs have been validated and evaluated. Overall, this article provides an overview of the chemical approach to DELs and their output.
Frequently Asked Questions
- How do DNA-compatible reactions ensure the potency of compounds in DEGL?
- DNA-compatible reactions maintain the chemical integrity and biological activity of compounds by optimizing reaction conditions. By controlling the reaction environment, degradation or loss of activity of the compound is avoided, thus ensuring that the compound maintains potency during the screening process.
- How do DNA-compatible reactions affect the accuracy of High-throughput Screening (HTS)?
- DNA-compatible reactions ensure that compounds are stable during the screening process, which reduces errors in screening results due to degradation or instability of compounds, thus increasing the reliability of the data. At the same time, false positives or false negatives due to non-specific interactions are reduced, resulting in more credible screening results.
At CD BioGlyco, we offer DNA-compatibility functional group transformation development services focused on, ensuring that your chemistry is perfectly compatible with the DEGL while maintaining high efficiency. Please feel free to contact us for the details of our services!
Reference
- Shi, Y.; et al. DNA-encoded libraries (DELs): a review of on-DNA chemistries and their output. RSC advances. 2021,11(4):2359-76.
For research use only. Not intended for any clinical use.
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