Overview of Light Crosslinking DEGL

Nowadays, light crosslinking probes have become an effective tool for capturing and elucidating biomolecular interactions. Light crosslinking DNA-encoded glycan library (DEGL)-based screening strategies have greatly improved the efficiency of discovering bioactive ligands in complex physiological environments. Thus, light crosslinking DEGL is an innovative research tool that not only provides great chemical diversity in high-throughput screening but also improves intermolecular binding efficiency and specificity through photocrosslinking technology.

At CD BioGlyco, we provide professional light crosslinking DEGL construction services. Our team has extensive experience in DEGL construction and cutting-edge biochemistry, and we design and synthesize light crosslinking DEGLs according to our client's specific research needs.

Illuminate Glycan Discovery with Light Crosslinking DEGL

We provide custom light crosslinking DEGL constructs to our clients, ensuring that each glycosyl molecule is efficiently linked and crosslinked to the corresponding DNA barcodes through sophisticated chemical reactions, providing strong technical support for our client's research.

Preparation of Light Crosslinking Glycans

We select specific glycan molecules based on clients' research objectives and introduce light crosslinking groups to their structures to obtain light crosslinking glycan compounds. In other words, light crosslinking glycan compounds are obtained by introducing light crosslinking probes on the surface of the glycan.

For example, the introduction of an aryl nitrostyrene group to the terminal position of glucose results in a glucose molecule with light crosslinking properties.

Light crosslinking groups typically contain aryl nitrostyrene vinyl groups, azide groups, azobenzene, spiropyran, and others. We will selectively introduce light crosslinking groups to the surface of the glycan according to the specific experimental requirements.

Construction of Light Crosslinking DEGL

• Firstly, based on the client's requirements, our team designs and synthesizes unique DNA barcodes. In this process, we provide the design and synthesis of DNA barcodes of different lengths and sequence complexity, ensuring high diversity and randomness to cover a wide range of samples and preventing similarities between different barcodes from leading to identification errors during subsequent experiments.
  • In addition, we offer a wide range of DNA barcode synthesis methods for clients to choose from, including solid-phase synthesis, polymerase chain reaction (PCR) amplification, and so on. The synthesized DNA barcodes will be sequenced and verified to ensure the sequence is correct and complete.
• The light crosslinking modified glycosyl molecules are then attached to the corresponding DNA barcodes by coupling techniques, DNA-compatible reactions, or click chemistry. For example, a glucose molecule with an aryl nitrostyrene vinyl group is attached to its specific DNA sequence barcode by a click chemistry reaction. Each of these barcodes represents a specific glycan molecule. A DNA-coded glycan molecule is placed under ultraviolet (UV) light irradiation to initiate a light crosslinking reaction, which causes the glycan molecule to form a covalent linkage with the target gene or protein to achieve DNA labeling of the target protein. For example, glucose with a DNA barcode attached is treated under UV light to cross-link it to the target protein.
• Finally, the above steps are repeated to design separate DNA barcodes for several different glycans (e.g., galactose, mannose, etc.), perform ligation and cross-linking reactions, and construct a diverse light crosslinking DEGL.
  • We test the functionality of the obtained light crosslinking DEGL using an affinity screening strategy to ensure that it meets the client's needs. We also provide High-throughput Screening for light crosslinking DEGLs for a wide range of molecules, such as glycoproteins, antibodies, or enzymes.

Workflow

Light crosslinking DEGLs have important applications in glycobiology and drug discovery, enabling systematic screening, characterization, and optimization of the activity of glycan compounds to identify potential drug targets. Our light crosslinking DEGL construction process will assist you in better understanding and applying light crosslinking DEGL.

Applications

• Light crosslinking DEGL efficiently screens light-sensitive glycan molecules, thus helping researchers develop biosensors for disease research and environmental monitoring.
• Light crosslinking DEGL enables the identification of disease-related glycan molecule biomarkers.
• Light crosslinking DEGL can be used to screen specific glycan molecules, accelerating the development of glycoprotein vaccines and glycan adjuvants.

Advantages

• Our team has mastered the most advanced DNA coding technologies and efficiently and accurately carries out the design and synthesis of DNA barcodes, and the construction of light crosslinking DEGLs. These technologies ensure that the constructed light crosslinking DEGLs are highly versatile and accurate, thus improving the reliability of the screening results.
• We offer a full-flow, high-throughput screening platform capable of screening a large number of compounds in a short period using light crosslinking DEGLs.
• We offer a wide range of levels of light crosslinking DEGLs to ensure that our clients get the libraries that best meet their scientific needs and achieve higher efficiency and success rates in their subsequent work.

Publication Data

Frequently Asked Questions

• How many different glycans can a light crosslinking DEGL library contain?
  • The content and size of light crosslinking DEGL libraries can be customized according to requirements and can typically contain hundreds of thousands or even tens of millions of glycan molecules.
• What are the precautions when constructing a light crosslinking DEGL?
  • Ensure the uniqueness and stability of the DNA barcode sequence.
  • Optimise light crosslinking conditions to avoid non-specific cross-linking.
  • Avoid physical and chemical interference between glycans and DNA barcodes.

CD BioGlyco uses light crosslinking technology to combine different glycan molecules with unique DNA barcodes to provide custom light crosslinking DEGLs to clients, helping them achieve their goals of diverse and efficient screening of glycosylated molecules. We welcome clients to feel free to contact us to discuss and promote the application and development of light crosslinking DEGL technology.

• DNA-encoded Single-Pharmacophore Glycan Library
• DNA-encoded Dual-Pharmacophore Glycan Library
• DNA-encoded Trio-Pharmacophore Glycan Library
• Solid Phase-based DNA-encoded Glycan Library (DEGL)
• Liquid Phase-based DNA-encoded Glycan Library (DEGL)
• DNA-encoded Natural Glycan Library
• DNA-encoded Modified Glycan Library
• DNA-encoded Glycan Antigen Library
• DNA-encoded Glycopeptide Library
• Monovalent DNA-encoded Glycan Library (DEGL)
• Multivalent DNA-encoded Glycan Library (DEGL)
• Scaffold-based DNA-encoded Glycan Library (DEGL)
• Target-based DNA-encoded Glycan Library (DEGL)
• DNA-encoded Glycan Macrocycle Library
• DNA-encoded Glycan Covalent Inhibitor Library
• DNA-encoded Glycoprotein Degrader Library
• DNA-encoded Glycan Allosteric Inhibitor Library
• DNA-encoded Dynamic library (DEDL)
• Coupling Reaction-based DNA-encoded Glycan Library (DEGL)
• Multi-component Reaction-based DNA-encoded Glycan Library (DEGL)
• Photocatalytic Cycloaddition-based DNA-encoded Glycan Library (DEGL)
• Aromatic Heterocyclization-based DNA-encoded Glycan Library (DEGL)
• Non-aromatic Heterocyclization-based DNA-encoded Glycan Library (DEGL)
• Carbocyclization-based DNA-encoded Glycan Library (DEGL)
• Macrocyclization-based DNA-encoded Glycan Library (DEGL)
• Click Chemistry-based DNA-encoded Glycan Library (DEGL)