Overview

CD BioGlyco has extensive experience in customized DNA-encoded glycan libraries (DEGLs) and has helped to study various glycobiological studies such as Glycan-protein Interactions, and bacterial enzymes involved in glycan biosynthesis through High-throughput Screening of DEGLs. Bacteria contain a variety of glycans, such as peptidoglycan. Enzymes involved in their biosynthesis are a rich source of targets for novel antibacterial agents. We provide a professional and customized DEGL screening service for bacterial enzyme research, helping to screen inhibitors of bacterial enzymes by designing and constructing DEGLs to support the development and optimization of antimicrobial substances. Besides, we also help to analyze the binding specificity of bacterial enzymes, glycan recognition, targets for bacterial infection intervention, etc.

Help Accelerate Bacterial Enzyme Inhibitor Development Through DEGL Screening

Based on our leading synthetic capabilities and experienced team, we provide customized solutions, including DEGLs tailored to specific bacterial enzymes, DEGL screening, Data Analysis, and Hit Validation and Assessment. The specific experimental steps are as follows:

Library Design

We design DEGLs for various bacterial enzyme targets, taking into account the natural substrates of the enzyme, potential binding targets, complexity of glycans, etc.

Library Construction

We custom-sterilize libraries of various orders of magnitude according to our clients' bacterial enzyme research needs. Each glycan structure is conjugated to a unique DNA tag, which serves as a unique identifier for the glycan and is used to track and identify glycan interactions with bacterial enzymes.

Library Screening

The constructed DEGL is used to screen high-affinity molecules against bacterial enzymes in a high-throughput manner, including affinity-based screening. This process includes the incubation of bacterial enzymes with DEGL, washing, amplification, and sequencing.

• The bacterial enzyme is immobilized on solid beads and incubated with DEGL under conditions that support enzyme activity.
• The beads are washed to remove low affinity molecules and high affinity molecules are enriched.
• Hit compounds are confirmed by amplification and next-generation sequencing (NGS).

Data Analysis and Hit Evaluation

We analyze screening data using efficient bioinformatics tools and data processing algorithms with high data processing speed. Based on our leading synthetic capabilities, de-DNA compounds are also synthesized and their biological activities are validated through binding studies, structural analyses, and more. We help screen bacterial enzyme inhibitors efficiently and provide support for the development of effective treatments for bacterial infections.

Workflow

After understanding our clients' experimental needs, we customize DEGL screening protocols to help find effective bacterial enzyme inhibitors and other studies.

Applications

• Antimicrobial development: DEGLs are used to screen bacterial enzyme inhibitors to identify potential drug lead compounds, thereby facilitating the design and development of novel antimicrobial agents.
• Drug targeting: Analysing bacterial enzyme binding molecules by DEGL screening facilitates the rapid study of ligand-target interactions and provides valuable information for drug targeting studies for the treatment of bacterial infections.
• Therapeutic approach development: The study of bacterial enzymes by DEGL screening provides valuable information for the development of effective therapeutic approaches for bacterial infections.

Advantages

• We customize DEGLs for specific glycan structures or bacterial enzyme types of interest to improve screening efficiency.
• We help researchers analyze bacterial enzymes efficiently and find effective inhibitors through flexible customized experimental protocols.
• We have efficient bioinformatics tools to rapidly analyze screening data and improve the efficiency of research projects.

Publication Data

Frequently Asked Questions

• What are the advantages of using the DEGL for high-throughput screening in bacterial enzyme inhibitor research?
  • The DEGL contains a range of different compounds, providing a wide range of chemical structures for exploring potential bacterial enzyme inhibitors and increasing the likelihood of discovering new lead compounds. Based on this, researchers can target specific bacterial enzymes of interest and thus discover inhibitors against specific enzymes or pathways. In addition to this, DEGL screening enables high-throughput screening of billions of compounds in a single experiment. This high-throughput property also accelerates the process of antibacterial agent discovery.
• Can DEGL be customised?
  • Of course, it can, DEGL is customized according to the client's research objectives and the type of bacterial enzyme. After understanding the experimental needs of the client, we synthesize libraries of various orders of magnitude and incubate them with bacterial enzymes to screen for effective inhibitors.

CD BioGlyco offers a custom DEGL screening service to study bacterial enzymes and help screen for effective bacterial enzyme inhibitors. Please feel free to contact us for custom DEGL and screening. We look forward to working with you to reveal new pathways, mechanisms, or targets for bacterial infection intervention, opening the way for further research and therapeutic development.

• Magnetic Bead-based DEGL Affinity Screening
• Resin Tip-based DEGL Affinity Screening
• Interaction-dependent PCR (IDPCR)-based DEGL Screening
• DNA Photoaffinity Labeling-based DEGL Screening
• Microfluidic Processor-based DEGL Screening
• Cell-based DEGL Screening
• DNA Programmed Affinity Labelling (DPAL)-based DEGL Screening
• DEGL Screening for Glycan-Protein Interaction Study
• DEGL Screening for Protein-Protein Interaction Inhibitor Discovery
• DEGL Screening for G Protein-coupled Receptor-Binding Compounds Discovery
• DEGL Screening for Protein Ubiquitination Study
• DEGL Screening for Antibody Target Identification
• DEGL Screening for Protein-templated Ligand Discovery