Solid Phase-based DNA-encoded Glycan Library (DEGL)
Overview of Solid Phase-based DNA-encoded Glycan Library (DEGL)
DEGL is an advanced technique for target small molecule screening. Based on different synthetic phases, CD BioGlyco provides high-quality DEGL based on solid phase or Liquid Phase. CD BioGlyco provides DEGL Construction services by solid-phase synthesis on magnetic beads and self-purification. This method rapidly builds and screens large glycan libraries and accelerates the discovery of novel bioactive molecules.
DEGL: Dual-linker Solid Phase Synthesis for High-purity
CD BioGlyco provides DEGL construction services based on solid-phase synthesis followed by self-purifying release of the library. Our lab provides the synthesis of DELs with much larger chemical complexity. The first and last building blocks of each library member are attached to the magnetic beads via linkers that can be chemically cleaved orthogonally to each other.
DNA-encoded Solid-phase Synthesis on Magnetic Beads
- Our researchers attach the first linker to an amino-modified solid-phase carrier based on a trifunctional backbone. The other two functional groups are used to link chemical groups and DNA barcodes, respectively. Our staff has proven operational experience in backbone binding and split-and-pool cycles (synthesis, capping, and encoding). Our researchers attach the second linker to the last chemical building block, providing the second covalent linkage for the solid phase support.
- Our researchers cleave the first linker and then wash all capped truncations. The desired product is obtained when the compound attached to the second linker is released by cleavage. Our researchers sequentially cleave and wash the first and last linkers to ensure that the final library contained only intact compounds to greatly increase purity.
Self-purifying Release from Magnetic Beads
Rely on the use of two orthogonally cleavable traceless linkers, CD BioGlyco provides professional self-purifying release from magnetic beads. Interestingly, DNA-compatible synthesis on magnetic beads enables a variety of chemical transformations. Our researchers provide customized chemical modification solutions for clients.
Workflow
Combining solid-phase synthesis and DNA encoding methods, our glycan library construction experts deliver satisfactory DEGLs. We help our clients generate diverse libraries of glycan compounds to meet their client's needs. Depending on the client's needs, we offer specialized chemical modification services.
Applications
- Solid phase-based DEGL can be used for glycan-related drug discovery and glycosylation pathogen identification.
- Solid phase-based DEGL plays an important role in the field of glycosylation biology and glyco-pharmaceutical development.
- Solid phase-based DEGL can be used to identify potential bioactive molecules.
- Solid phase-based DEGL can be used to recognize lower affinity ligands.
Advantages of Us
- Our solid-phase synthesis reaction provides easy purification and shortens the production cycle for synthesizing DEGL.
- Our researchers perform synthesis on magnetic beads to favor amide bonding.
- The libraries we synthesize using solid phase have fewer by-products with high purity.
Publication Data
Technology: DEAE Sepharose columns, Reverse-phase high-performance liquid chromatography (HPLC), Sequencing, Primer extension assay, Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)
Journal: PLoS Biology
Published: 2004
IF: 8.2
Results: In this study, researchers present a new method for solid-phase organic synthesis on unprotected DNA. Complex organic molecules are synthesized by performing a series of chemical reactions on a solid-phase carrier on a DNA strand. Using a resin as a solid-phase carrier, the researchers performed various organic synthesis steps, such as alkylation, acylation, and cyclization reactions, on unprotected DNA sequences. Validation has shown that this method can efficiently generate large libraries of DNA-encoded organic compounds.
Fig.1 Schematic structure of DNA support structure and modified amino acids. (Halpin, et al., 2004)
Frequently Asked Questions
- Is there a relationship between the size of the beads and the construction of the library?
- The number and diameters of the magnetic beads are directly related to the volume of the library. The number of magnetic beads at least matches the desired library size. For example, 2 μm magnetic beads easily withstand a library size of billions.
- What are the advantages of solid-phase synthetic libraries?
- Solid-phase synthesis schemes offer advantages in anhydrous DNA-compatible transformations. DNA-compatible synthesis on magnetic beads enables a variety of chemical transformations such as heterocyclic and spirocyclic DELs with high structural diversity.
CD BioGlyco combines solid-phase synthesis with selective self-purification release of DEGL to provide our clients with high-purity DEGL construct services. Our experts are dedicated to meeting all the problems you encounter in DEGL development. Feel free to contact us for any reason whatsoever.
Reference
- Halpin, D.R.; et al. DNA display III. Solid-phase organic synthesis on unprotected DNA. PLoS Biology. 2004, 2(7): e175.
For research use only. Not intended for any clinical use.
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