Overview of Multivalent DNA-encoded Glycan Library (DEGL)

At CD BioGlyco, we are committed to promoting technological innovations in the field of glycomics and glycoprotein interactions such as DEGL. Among them, our multivalent DEGL construction service accelerates the discovery and analysis of glycan structures and their biological interactions by creating highly diverse glycan libraries encoded with unique DNA sequences. This enables high-throughput screening and detailed structural function analysis. This service is tailored to the specific research needs of clients, providing libraries ranging from thousands to tens of millions of different glycan structures.

Dive into Multivalent DEGL and Revolutionize Your Glycan Research

The construction of multivalent DEGL is a highly complex and sophisticated process that combines the multidisciplinary knowledge of combinatorial chemistry, molecular biology, and bioinformatics. The technology uses a unique DNA barcode to give each glycan molecule an identity. The polymerase chain reaction (PCR) amplification and next-generation sequencing technology are used to achieve efficient identification of the result of high-throughput screening.

Connection of DNA Barcodes with DNA Starting Material

During the process, we use advanced biological methods to connect DNA tags to the DNA starting material. This step is the key to constructing multivalent DEGL, which ensures a stable connection between DNA tags and DNA starting material.

Combinatorial Chemical Synthesis

We perform large-scale on-DNA glycan synthesis using the "split and pool" strategy in combinatorial chemistry. In each synthesis cycle, we introduce different glycan reagents to generate glycan molecules with specific structures and diversity by precisely controlling the reaction conditions. Once the 1-cycle compound library has been purified and re-dissolved, it is divided, and the selected glycan undergoes the same operation for an on-DNA conjugation reaction.

Library Expansion and Customization

Our synthetic platform is highly flexible and scalable, enabling the synthesis of millions to tens of millions of compounds in a library tailored to client needs. By adjusting the synthesis parameters and reaction conditions, we achieve precise control of the glycan structure, length, and connection mode, and provide customized multivalent DEGL services to clients.

Quality Control and Verification

During the construction process, we use a variety of methods to control and verify the quality of multivalent DEGL including mass spectrometry (MS) analysis and spectroscopy analysis, liquid chromatography analysis, etc. to ensure the structural correctness and diversity of glycan molecules in the library.

Workflow

Applications of Multivalent DEGL

• Glycan array development: Researchers can develop glycan arrays that mimic the complexity of natural glycan displays on cell surfaces by constructing a diverse library of multivalent glycans.
• Glycan-based drug discovery: The DEGL serves as a rich source of potential glycan ligands for drug development. Researchers can design glycan-based inhibitors or modulators to alter protein function by identifying glycans that bind with high affinity to disease-related proteins.
• Glycan-based biomarker discovery: The DEGL can be used to identify glycan signatures that are associated with specific cellular states or disease conditions.

Advantages

• Highly customized solutions: We are good at customizing the construction of the multivalent DNA-encoded glycan library according to client needs.
• Efficient screening and identification capabilities: Our DEGL achieves rapid screening and accurate identification of massive glycan-protein interactions by integrating high-throughput screening technology and advanced DNA decoding technology.
• Comprehensive technical support and services: We are well aware of the various challenges that clients may encounter in the process of using DEGL, so we provide a full range of technical support and services. From experimental design to data analysis, our professional team accompanies clients throughout the process to ensure the smooth progress of research projects.

Publication

Frequently Asked Questions

• What is multivalent DEGL?
  • Multivalent DEGL is a multivalent glycan library constructed using DNA encoding technology. This technology combines the encoding ability of DNA with the biological activity of glycans, and labels each glycan molecule through the uniqueness of the DNA sequence, allowing millions of different glycan structures to be tested simultaneously in high-throughput screening. DEGL has broad application prospects in drug discovery, vaccine development, and biomarker identification, and significantly improves research efficiency and success rate.
• Why choose the multivalent DEGL?
  • The main reason for choosing multivalent DEGL is that it greatly expands the screening range of glycan compounds. Traditional methods are difficult to synthesize and screen such a large number of glycan molecules, and DEGL technology makes it possible to construct and screen a large number of multivalent glycan molecules through DNA encoding. In addition, DEGL is also highly scalable and adjusts the structure and diversity of glycan molecules according to research needs, to discover more potential candidate molecules.

With our outstanding technical strength and customized service concept, CD BioGlyco is committed to providing high-quality multivalent DEGL construction services to researchers around the world. Our joint efforts and innovations will reveal more mysteries of glycans and promote the continuous progress and development of the field of life sciences. Please feel free to contact us for more details if you are interested in our multivalent DEGL construction service!

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