Overview of Next-generation Sequencing (NGS) of the High-throughput Screening (HTS) Output

The DNA-encoded glycan library (DEGL) combines DNA coding sequences with a large number of glycans. Unique DNA tags attached to each glycan enable rapid, parallel screening of millions of interacting compounds simultaneously. This enables efficient analysis of large numbers of samples, thereby accurately identifying molecules with specific biological activities in complex systems.

At CD BioGlyco, we have professional library experts to provide clients with high-quality Library Design, Construction, Screening, sequencing, Data Analysis, and Validation services. Whether you need Natural Glycan Libraries, Modified Glycan Libraries, or Carbohydrate Antigen Libraries, we provide you with the most satisfactory customized libraries. Using NGS, we optimize the process of decoding DNA and improve the accuracy of DNA sequencing.

Crack the Code Using Our Advanced NGS

We perform enzymatic reactions and data acquisition in a coordinated and stepwise execution that allows sequencing data to be generated from tens of thousands to billions of templates simultaneously. NGS is capable of performing sequence reads on hundreds of thousands to millions of DNA molecules at a time in parallel. Our researchers use NGS for deep sequencing of DEGL to analyze glycan selection and binding diversity.

Sequencing by Synthesis (SBS)

• Before sequencing, our researchers use physical methods to cut DNA samples from glycan libraries into fragments. The splice sequences on the fragments are covalently linked to complementary oligonucleotides, which are then amplified.
• Our state-of-the-art sequencer sequences many fragments simultaneously reads out the fluorescent color information in each fragment in real time and then reconstructs the original DNA sequence through software methods.

Detection and Optimization

• Our lab provides direct fluorescence detection, indirect fluorescence detection, and synthesis-detection combination services. Our researchers monitor manual mixtures of DNA coding during polymerase chain reaction (PCR) amplification by NGS to examine potential biases.
• Our goal is to improve sequencing accuracy through rational design, fluorescent labeling, synthesis of protected unnatural dNTP, a new DNA polymerase, new surface chemistry, and a method to locally amplify unique copies of DNA fragments.

Workflow

NGS plays a key role in the analysis of DEGL. The NGS workflow for DEGL typically includes the following steps:

Applications

• NGS can be used for high-throughput analysis of differential interactions of glycan molecules, facilitating in-depth research on glycobiology.
• NGS plays an important role in screening and analyzing high-volume glycan libraries to accelerate the analysis process.
• NGS can be used for sequencing glycan libraries from source tumor tissues, detecting tumor-associated glycan gene mutations, changes in glycan expression profiles, etc., and guiding tumor typing, prognostic assessment, and targeted therapy.
• NGS can be used to study microbial glycan library detection and assess glycan species composition and abundance in environmental samples.

Advantages of Us

• The NGS we provide enables high-throughput sequencing by processing a large number of DNA fragments in parallel in a short period, greatly reducing the sequencing cycle.
• The NGS provides that sequences millions or even billions of DNA molecules at the same time, greatly improving sequencing efficiency.
• Relying on a team of computer specialists, we offer sequencing data interpretation, integration, and visualization.

Publication Data

Frequently Asked Questions

• What is the principle of NGS?
  • The main principles of NGS are based on DNA synthesis and optical signal detection. During the sequencing process, the DNA sample is first broken into smaller fragments, and then these fragments are ligated into a vector to form a DNA library. The DNA fragments in the library are then amplified by PCR to produce a large number of sequences of the same fragment. These DNA fragments are then immobilized on the surface of the solid phase vector and subjected to a sequencing reaction. During the sequencing reaction, the DNA fragments are synthesized one by one, one base at a time, and a fluorescent signal is released, which is captured by a detector recorded, and analyzed to be converted into appropriate information for our use.
• What are the advantages of next-generation sequencing?
  • One common feature of high-throughput sequencing technologies is that sequencing is highly parallelized, i.e., thousands of sequencing reactions can be performed simultaneously on a single platform and the reaction system is very small, yielding a large amount of base information in a very short period. Next-generation sequencing has higher accuracy, shorter read lengths, and high throughput. Importantly, the sequence coverage of next-generation sequencing is very high.

CD BioGlyco is a leading provider of NGS and is dedicated to advancing glycan library development. Our state-of-the-art facilities are equipped with the latest sequencing technologies, ensuring that our clients receive the most accurate and comprehensive data available. For any further information, do not hesitate to contact us.

• DNA-encoded Glycan Library (DEGL) Design
• DNA-Compatible Reaction Development
• DNA-encoded Glycan Library (DEGL)
• High-throughput Screening (HTS) of DNA-encoded Glycan Library (DEGL)
• Data Analysis and Visualization
• Hit Validation and Assessment