Glycoinformatics-assisted Identification Service of Genes Involved in Glycan Biosynthesis

Glycoinformatics-assisted Identification Service of Genes Involved in Glycan Biosynthesis

Simplifying the Identification of Genes Involved in Glycan Biosynthesis Through Customized Protocols

Bioinformatics analysis simplifies the process of analyzing gene sequencing data, allowing more time to be devoted to subsequent glycan biosynthesis studies. CD BioGlyco offers a comprehensive, professional, and customized glycoinformatics-assisted identification service of genes involved in glycan biosynthesis based on our extensive experience in Transcriptomics Analysis. We utilize advanced analytical tools to help you gather meaningful information and accurately analyze glycan biosynthesis more easily and quickly.

Our team consists of researchers with rich experience and knowledge in bioinformatics and Glycomics Analysis. Depending on the client's research objectives, we customize specific experimental protocols and dig deep into the data. Combined with advanced analytical techniques and software, our service mainly includes the following components:

RNA sequencing and analysis

This service includes comprehensive RNA sequencing and specialized bioinformatics analysis based on the sequencing data. We use advanced software to help researchers extract and interpret glycan biosynthesis information from RNA sequencing data and identify genes involved in glycan biosynthesis. Regulation of glycan biosynthesis is complex. We mine mRNA transcription data to better understand glycan biosynthesis networks and identify hotspots of genomic regulation.

RNA sequencing: We offer a full range of RNA sequencing services to help analyze gene expression patterns, examine changes in the transcriptome, and analyze genes involved in glycan biosynthesis. Our sequencing technologies offer the advantages of high sensitivity, high throughput, and high speed to accelerate experimental processes.

Gene expression analysis: We use bioinformatics methods to differentially analyze gene expression in data generated by RNA sequencing and identify genes involved in glycan biosynthesis.

Gene annotation and functional analysis: We combine multiple databases to analyze the functions played by each gene in glycan biosynthesis and understand the genetic basis of glycan diversity.

Mass spectrometry (MS)-based gene identification

We construct defective mutants of various putative genes involved in glycan biosynthesis and analyze glycans by MS. The genes involved in glycan biosynthesis are identified by comparing the differences in glycans in mutants and controls.

Gene validation

We also provide validation services to confirm the function of the identified genes involved in glycan biosynthesis. This process involves constructing mutants through gene overexpression, gene knockout, and other techniques and performing glycan assays to understand gene function. We have a variety of high-quality models for validation.

Comprehensive and professional glycoinformatics-assisted identification service of genes involved in glycan biosynthesis. (CD BioGlyco)

Publication Data

Technology: MS

Journal: Genes

IF: 3.759

Published: 2011

Results: In this study, the researchers identified glycosylation-related genes in Pseudomonas syringae pv. tabaci 6605 (Pta 6605) by constructing genetically defective mutant strains and investigating the molecular mass of flagellin and its glycopeptides. By analyzing the flagellin glycans of the mutant strain using MS, the researchers newly identified vioR and vioM, biosynthesis genes for modified viosamine (mVio) glycosylation in this bacterium. The analysis also revealed that mVio-related genes are essential for mVio biosynthesis in flagellin glycans. This study provides guidance for us to identify glycan biosynthesis-related genes.

Fig.1 The vioM and vioR mutant strains and viosamine island in Pta 6605.Fig.1 Viosamine island and generation of mutant strains. (Yamamoto, et al., 2011)

Applications

  • Identification of genes involved in glycan biogenesis provides valuable information for understanding glycan biosynthesis and identifying hotspots of genomic regulation.
  • The identification of genes involved in glycan biogenesis lays the foundation for the regulation of glycan synthesis pathways.
  • The identification of genes involved in glycan biogenesis helps to compare gene expression differences in various diseases, thus laying the foundation for the development of therapeutic approaches to diseases.

Advantages

  • We utilize advanced software to simplify the process of analyzing RNA sequencing data and shorten analysis cycles to accelerate the analysis process for researchers.
  • We integrate multiple data related to glycan biosynthesis to ensure a comprehensive understanding of the process and associated genes.
  • We systematically analyze genes involved in glycan biosynthesis and identify the keys involved in glycan biosynthesis.

Frequently Asked Questions

  • What is the significance of identifying genes involved in glycan biosynthesis?
    • Many studies show that glycans have a variety of biological activities, including involvement in communication between cells and the external environment, development of multicellular organisms, and other aspects. More and more researchers are focusing on the role of glycans in life processes and their biosynthesis. The identification of genes involved in glycan biosynthesis helps to deeply analyze the mechanisms of glycan biological functions, physiological and pathological processes, etc.
  • Which databases are commonly used to identify genes in glycan biosynthesis?
    • Some glycobioinformatics-related databases play an important role in identifying genes for glycan biosynthesis. These databases cover the structure of sugar chains, genes involved in glycan synthesis, enzymes, metabolic pathways, and results of glycomics experiments.
  • Is experimental validation necessary after identifying potential genes involved in glycan biosynthesis?
    • Yes, experimental validation is essential to confirm the function of identified genes in the glycan biosynthesis pathway. We provide a one-stop service for gene validation. We validate the role of specific genes in glycan biosynthesis through gene knockout, overexpression, and glycan analysis.

CD BioGlyco offers a comprehensive glycoinformatics-assisted identification service of genes involved in glycan biosynthesis utilizing state-of-the-art technology and techniques. Our goal is to provide flexible, scalable, and innovative solutions to meet our clients' research needs. Please feel free to contact us to discuss your specific experimental program if you need this area.

Reference

  1. Yamamoto, M.; et al. Identification of genes involved in the glycosylation of modified viosamine of flagellins in Pseudomonas syringae by mass spectrometry. Genes. 2011, 2(4): 788-803.
For research use only. Not intended for any diagnostic use.
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