Bioinformatics analysis simplifies the process of analyzing gene sequencing data, allowing more time to be devoted to subsequent glycan biosynthesis studies. CD BioGlyco offers a comprehensive, professional, and customized glycoinformatics-assisted identification service of genes involved in glycan biosynthesis based on our extensive experience in Transcriptomics Analysis. We utilize advanced analytical tools to help you gather meaningful information and accurately analyze glycan biosynthesis more easily and quickly.
Our team consists of researchers with rich experience and knowledge in bioinformatics and Glycomics Analysis. Depending on the client's research objectives, we customize specific experimental protocols and dig deep into the data. Combined with advanced analytical techniques and software, our service mainly includes the following components:
This service includes comprehensive RNA sequencing and specialized bioinformatics analysis based on the sequencing data. We use advanced software to help researchers extract and interpret glycan biosynthesis information from RNA sequencing data and identify genes involved in glycan biosynthesis. Regulation of glycan biosynthesis is complex. We mine mRNA transcription data to better understand glycan biosynthesis networks and identify hotspots of genomic regulation.
RNA sequencing: We offer a full range of RNA sequencing services to help analyze gene expression patterns, examine changes in the transcriptome, and analyze genes involved in glycan biosynthesis. Our sequencing technologies offer the advantages of high sensitivity, high throughput, and high speed to accelerate experimental processes.
Gene expression analysis: We use bioinformatics methods to differentially analyze gene expression in data generated by RNA sequencing and identify genes involved in glycan biosynthesis.
Gene annotation and functional analysis: We combine multiple databases to analyze the functions played by each gene in glycan biosynthesis and understand the genetic basis of glycan diversity.
We construct defective mutants of various putative genes involved in glycan biosynthesis and analyze glycans by MS. The genes involved in glycan biosynthesis are identified by comparing the differences in glycans in mutants and controls.
We also provide validation services to confirm the function of the identified genes involved in glycan biosynthesis. This process involves constructing mutants through gene overexpression, gene knockout, and other techniques and performing glycan assays to understand gene function. We have a variety of high-quality models for validation.
Technology: MS
Journal: Genes
IF: 3.759
Published: 2011
Results: In this study, the researchers identified glycosylation-related genes in Pseudomonas syringae pv. tabaci 6605 (Pta 6605) by constructing genetically defective mutant strains and investigating the molecular mass of flagellin and its glycopeptides. By analyzing the flagellin glycans of the mutant strain using MS, the researchers newly identified vioR and vioM, biosynthesis genes for modified viosamine (mVio) glycosylation in this bacterium. The analysis also revealed that mVio-related genes are essential for mVio biosynthesis in flagellin glycans. This study provides guidance for us to identify glycan biosynthesis-related genes.
Fig.1 Viosamine island and generation of mutant strains. (Yamamoto, et al., 2011)
CD BioGlyco offers a comprehensive glycoinformatics-assisted identification service of genes involved in glycan biosynthesis utilizing state-of-the-art technology and techniques. Our goal is to provide flexible, scalable, and innovative solutions to meet our clients' research needs. Please feel free to contact us to discuss your specific experimental program if you need this area.
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We envision a future where the intricate world of carbohydrate is no longer shrouded in mystery, but rather illuminated by the power of cutting-edge computational tools.