Glycoinformatics-assisted Mannosylation Site Prediction Service
Unlocking Glycoprotein Secrets: Advanced Mannosylation Site Prediction
Glycosylation is a very important post-translational modification that has a significant impact on protein function and stability. Among them, mannosylation is a rare form of glycosylation, and its importance in protein research is gradually recognized and valued. With the development of informatics technology, the Prediction of the Structure and Function of biomolecules has gradually become mature. As a professional company conducting biological research, CD BioGlyco has mastered advanced glycoinformatics technology to provide clients with mannosylation site prediction services.
Obtaining protein sequence data
From the literature and various protein databases, we collect data such as sequence information for different kinds of natural proteins as well as naturally occurring or engineered mutants. In this, we annotate experimentally verified mannosylation sites and unmodified sites.
Submit sequences and analyze the results
We input the sequences of the proteins that our clients need to predict into the informatics tool, which provides prediction results based on internal algorithms and databases.
Sequence pattern matching: Search based on known mannosylation sequence patterns.
Machine learning methods: Using machine learning algorithms such as support vector machines and neural networks to train models based on known glycosylation site data to predict the mannosylation sites of unknown sequences.
Structure prediction: Combining protein 3D structure information, we predict the spatial accessibility of the mannosylation sites and potential mannosylation structures.
In this process, our prediction tool displays the scores of mannosylation-specific sites to show the magnitude of the likelihood of mannosylation.
Validation results
To validate the predicted results of the mannosylation sites, we conduct further laboratory research, such as using mass spectrometry to test the accuracy of the predicted mannosylation sites. In addition, by comparing known mannosylation modification standards with predicted modification sites, clients gain a more accurate interpretation of the function and regulatory mechanisms of mannosylation modifications.
Applications
- Glycoinformatics-assisted mannosylation site prediction can be used to predict and optimize mannosylation modifications of pharmaceutical proteins or antibodies, thus helping researchers to design biological drugs with mannosylation patterns to enhance their therapeutic effects or targeting.
- By predicting mannosylation sites, researchers in the field of medicine construct a rapid understanding of the function of mannose modifications in biological processes and also assist them in predicting protein structures, especially the three-dimensional structures of glycoproteins.
- The prediction of protein mannosylation sites helps researchers to understand the effects of mannosylation on cellular functions and to more accurately interpret the functions and regulatory mechanisms of mannosylation modifications.
Advantages
- We use advanced machine learning and algorithms to process and analyze complex glycosylation data to ensure that our clients' mannosylation site prediction requirements are met.
- We have experts in many fields, including biology, chemistry, informatics, and engineering, forming a strong interdisciplinary team to provide personalized mannosylation site prediction services and data analysis reports according to client requirements.
- We provide an integrated platform that facilitates one-stop operation from data entry to analysis of mannosylation site prediction results.
Frequently Asked Questions
- What is mannosylation?
- Mannosylation is a specific protein glycosylation process in which mannose molecules are covalently attached to amino acid residues of a protein. Glycosylation is a crucial modification step in a variety of biological processes that involves the addition of sugar moieties to protein protons or other molecules, affecting their structure, function, and stability. Mannosylation typically occurs in systems such as the endoplasmic reticulum and the Golgi apparatus and is key to many important biological functions and processes, including protein folding, intercellular interactions, and immune responses.
- How to verify the accuracy of prediction results?
- Predictions usually need to be validated by experimental means such as mass spectrometry, enzyme-linked immunosorbent assay, or other biochemical methods. In addition, statistical analysis of the results can be performed using statistical tools to help users assess the reliability of the predictions.
CD BioGlyco performs mannosylation site prediction by integrating bioinformatics, computational biology, and glycomics approaches. Our informatics-based Analysis and Prediction Services help our clients to deeply understand the diverse functions of glycosylation modifications in biological systems, and have a wide range of applications in medicine, pharmacy, and even the food industry. Please feel free to contact us if you are looking for a partner in the field of glycosylation site prediction, we will be your best partner.
Reference
- Zhao, X.; et al. Glypre: In silico prediction of protein glycation sites by fusing multiple features and support vector machine. Molecules. 2017, 22(11): 1891.
For research use only. Not intended for any diagnostic use.
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