Precision Glycan Enzymatic Synthesis: Powered by Glycominformatics

Glycoinformatics is a discipline that intersects glycoscience and bioinformatics. It studies and analyzes the structure, function, and interaction of glycans using computational tools, databases, and algorithms. Researchers use glycoinformatics to predict glycan functions, simulate glycan structures, and design glycan synthesis routes. CD BioGlyco is a professional glycobiology company dedicated to providing systematic and comprehensive glycan services to our clients to promote the development of glycoscience. We have a first-class Glycobioinformatics Platform to help clients use computational tools to reveal glycans in biological systems.

Glycoinformatics-guided Glycan Synthesis services utilize advanced computational tools and bioinformatics to design and produce complex glycans tailored for specific research or industrial applications. We offer two synthesis strategies: Glycoinformatics-guided Automated Glycan Synthesis and glycoinformatics-guided glycan enzymatic modular assembly.

Compared with chemical synthesis, enzymatic synthesis of glycans has the advantages of high reaction efficiency, few by-products, and mild conditions. CD BioGlyco applies glycoinformatics to the enzymatic synthesis of glycans, creating complex glycans in an efficient, accurate, and environmentally friendly way for application in the fields of vaccines, drugs, and material development. Computational tools help us analyze the structure of glycans, design glycan enzymatic synthesis routes, predict enzymatic reaction conditions, and monitor enzymatic reaction processes. Our services are as follows:

Design of Synthetic Route

Computational tools are used to analyze the structure of the target glycan; Design feasible enzymatic synthesis routes for the glycan (which enzymes are needed and in what order to add them) regarding the glycan database; Select specific enzymes that add or modify specific sugar residues; Predict enzymatic synthesis conditions.

Synthesis

Complex glycans are synthesized by using specific enzymes to add specific sugar residues to the glycan chain step by step according to the synthetic route, and the conditions of the enzymatic reaction are continuously optimized to improve efficiency and yield.

Quality Monitoring and Quality Control

The structure of glycans is monitored using techniques such as mass spectrometry (MS), nuclear magnetic resonance (NMR), and high-performance liquid chromatography (HPLC).

Publication

Applications

• Vaccine development: Glycobioinformatics-guided enzymatic synthesis is used to produce complex oligosaccharides that mimic the carbohydrate structures on the surface of pathogens. These glycans can be used as antigens in vaccines to elicit targeted immune responses.
• Drug development: Develop glycomimetics or glycosylated drugs based on complex glycans for the treatment of a variety of diseases.
• Material development: Glycobioinformatics-guided enzymatic synthesis is used to develop tissue engineering and biomaterials, which can be used in areas such as drug delivery.
• Glycan analysis: Through enzymatic synthesis of complex glycans, researchers develop tools for analyzing glycan expression patterns in various diseases and ultimately discover new biomarkers.
• Glycan microarrays: Synthesize a variety of complex glycans for microarrays to perform high-throughput screening of glycan interactions with proteins, cells, and other biomolecules.

Advantages of Us

• We apply glycoinformatics to the synthesis of glycans to help clients quickly analyze information about the structure, function, and biosynthetic pathway of glycans.
• With the help of computational tools, we help clients predict the enzymes and reaction conditions required for the synthesis of complex glycans.
• Enzymatic synthesis of glycans has high specificity and mild reaction conditions, reducing the production of side reactions.
• Our modular strategy allows the combination of different enzymatic steps to assemble a variety of complex glycans.
• Compared with chemical methods, enzymatic synthesis is more efficient and usually requires fewer steps and lower energy input.

Frequently Asked Questions

• What are the advantages of glycan enzymatic modular assembly compared to chemical synthesis?
  Enzyme-catalyzed reactions are specific and reduce the occurrence of side reactions; Enzyme-catalyzed reactions have milder reaction conditions; Glycan enzymatic reactions have fewer steps and are more efficient; Enzymatic reactions easily synthesize complex glycans with multiple branches and different connections; Enzymatic reactions have regional selectivity and stereoselectivity; Enzymatic modular assembly is easier to automate, thereby achieving high-throughput production.
• What are the common enzymes used for enzymatic synthesis of glycan?
  Glycosyltransferases: these catalyze the transfer of a sugar moiety from an activated donor to an acceptor molecule, such as glucosyltransferases, galactosyltransferases, fucosyltransferases, sialyltransferases. Glycosidases: these catalyze the hydrolysis of glycosidic bonds or are used in glycosynthase reactions to form glycosidic bonds, such as β-galactosidase, and β-glucosidase.

CD BioGlyco combines computational tools and enzymatic synthesis to provide an advanced, efficient, and versatile method for synthesizing complex glycans. If you are interested in our services, please feel free to contact us.

• Glycoinformatics-guided Automated Glycan Synthesis Service