Amplification-based Transcript Analysis Service
Unlocking the Impact of Glycosylation on Gene Expression Regulation
In life sciences, gene expression analysis is a major research tool studying the pathogenesis and progression of diseases as well as the mechanism of drug action. As a professional supplier in the field of glycomics, CD BioGlyco not only offers comprehensive
Glyconformatics-assisted Glycan-related Transcriptomics Analysis but also concentrates on the evaluation of the influence effect caused by glycosylation modifications to gene expression regulation characterization based on our integrated
Glyco-transcriptomics Transcript Analysis Service. Our service ranges from RNA extraction to amplification, sequencing, and data analyses are integrated with glycosylation modification analysis for a more holistic perspective on gene expression regulation mechanisms.
Several RNA extraction techniques are employed to guarantee that high-quality and sufficient amounts of RNA samples can be efficiently procured. We have different plans for RNA amplification, including in vitro transcription (IVT) and polymerase chain reaction (PCR) schemes to better fulfill the distinct requirements for diverse types of samples. The amplified RNA is sequenced using high-throughput methods to provide a comprehensive view of the transcriptome. An overview of the sequencing data, such as gene expression quantification, differential gene expression screening, and so on will be performed by our professional bioinformatics team. Notably, we incorporate glycosylation modification analysis to determine the influence of regulatory mechanisms induced by them on gene expression. Please get in touch with us to learn more about our
Glycoinformatics-assisted Glycomics Analysis Service.
Publication
DOI: 10.3390/targets2010001
Technology: Lectin, Microarray, Glycan recognization
Journal: Chemical Society reviews
Published: 2021
IF: 40.4
Results: This comprehensive review explores the pivotal role of cell surface glycans in diverse biological processes and underscores the necessity of in situ analysis to decipher their functional mechanisms. The review acknowledges the inherent challenges of glycans, including their structural complexity and scarcity, and delves into the strategies employed for their recognition, such as the use of lectins, aptamers, and antibodies. It then zeroes in on glycan labeling and sensing techniques that incorporate amplification strategies to enhance sensitivity and overcome these challenges. These techniques encompass metabolic labeling, chemoenzymatic labeling, and chemical covalent labeling, which are integrated with optical, electrochemical, and mass spectrometric detection modalities. The review concludes by delineating the future trajectory of glycan analysis, emphasizing the need for the development of more effective recognition molecules, enhanced spatial control of labeling processes, improved labeling efficiency, the establishment of standardized protocols, and the advancement of methods for dynamically tracking glycan changes.
Fig.1 Schematic diagram depicting the use of lectin-mediated RCA signal amplification method for in situ detection of exosomal glycans. (Jia, et al., 2014)
Applications
- Our service provides highly sensitive and accurate quantification of gene expression levels, enabling researchers to identify differentially expressed genes and understand the underlying biological processes.
- By analyzing the expression patterns of genes involved in specific pathways or biological processes, our service helps researchers gain insights into gene function and its role in complex systems.
- Our service can detect genetic variations, such as single nucleotide polymorphisms (SNPs) and insertions/deletions, providing valuable information for studies on genetic diversity and susceptibility to disease.
Advantages
- Our amplification-based approach ensures robust detection of low-abundance transcripts, minimizing the risk of false negatives.
- Our service is designed to handle large-scale transcriptomic data, enabling researchers to analyze hundreds or even thousands of samples simultaneously.
- Our team of experienced scientists and bioinformaticians offers expert support throughout the analysis process, from experimental design to data interpretation.
Frequently Asked Questions (FAQs)
- How does the amplification-based transcript analysis service work?
- This includes sample preparation, RNA extraction and purification, cDNA synthesis, and targeted amplification followed by high-throughput sequencing. The resulting data is then analyzed using our proprietary bioinformatics pipeline to identify differentially expressed genes and other relevant biological information.
- How do I get started with the amplification-based transcript analysis service?
- Contact us today to discuss your research goals and project requirements. Our team works with you to design a customized analysis pipeline and provide guidance on sample preparation and experimental design.
At CD BioGlyco, our amplification-based transcript analysis service helps clients delve into the mechanisms of gene expression regulation, particularly the impact of glycosylation modifications on gene expression, providing strong support for your scientific research. Please feel free to contact us for a personal quote and more information.
References
- Smyrlaki, I.; et al. Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR. Nature communications. 2020, 11(1): 4812.
- https://en.wikipedia.org/wiki/Sequence_analysis
- Jia, H.; et al, Cell surface-localized imaging and sensing. Chemical Society reviews. 2021, 50(10).
For research use only. Not intended for any diagnostic use.
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