Unraveling Gene Expression and Glycosylation Pathways with Precision - Empowering Discoveries in Glycobiology

CD BioGlyco has established a novel platform for Glycan-related Transcriptomics Analysis Service to investigate how gene expression regulates O-glycosylation of specific proteins. A hybridization-based RNA analysis combined with glycoinformatics would largely benefit from this method. Difference transcriptomic technologies such as gene/chip arrays and RNA sequencing (RNA-Seq) are used to analyze the transcripts of glycosylation pathways. The detailed pathway analysis was carried out using different bioinformatics tools and databases like GlycoGene DataBase (GGDB) and The Cancer Genome Atlas (TCGA). This was further confirmed by pathway analysis which showed various differentially expressed genes (DEG) for disease and glycosylation pathways, specific to either disease biomarkers or related molecular therapeutics. In addition, to offer more attractive services to our clients, Glycoinformatics-assisted Glycomics Analysis Services endeavor to unveil the epitome of glycoscience.

CD BioGlyco offers our advanced glycoinformatics-assisted glyco-transcriptomics transcript analysis service, which provides comprehensive insights into gene expression and glycosylation pathways. Our service leverages state-of-the-art technologies and a robust workflow to ensure accurate and detailed analysis.

Sample preparation

For biological samples, RNA is extracted and processed to remove rRNA and tRNA.

Library construction

cDNA libraries are created from the RNA extracts using selective probes for hybridization-based enrichment.

Sequencing and hybridization

The cDNA libraries are hybridized to DNA microarrays or sequenced on RNA-Seq platforms.

Data analysis

Transcriptomic data are further analyzed using bioinformatics tools for the identification of DEG followed by integration with glycomic data to perform pathway-related analysis.

Validation and interpretation

The findings are validated using qRT-PCR and MS, with the results interpreted to elucidate regulatory mechanisms of human protein glycosylation.

Publication

Applications

• Hybridization-based transcript analysis can be used to identify gene expression patterns associated with various diseases, such as cancer, diabetes, and infectious diseases.
• Hybridization-based transcript analysis can be used to investigate the regulation of metabolic pathways and their alterations in disease states.
• Hybridization-based transcript analysis can be used to examine how pathogens alter host gene expression during infection to identify potential therapeutic targets and biomarkers.
• Hybridization-based transcript analysis can be used to identify DEG that can serve as biomarkers for early cancer detection and prognosis.

Advantages

• This service is capable of detecting low-abundance transcripts and providing high-specificity results due to the use of specific probes for hybridization.
• Our service allows for the analysis of the entire transcriptome, including coding and non-coding RNAs.
• More cost-effective than some sequencing-based approaches, making it accessible for large-scale studies.

Frequently Asked Questions (FAQs)

• How does CD BioGlyco ensure the accuracy and reliability of the hybridization-based transcript analysis results?
  • Our service meticulously executed numerous quality control steps throughout the process from RNA extraction to data analysis. These are validation of the expression data from two gold-standard techniques such as qRT-PCR and MS. We have extensive experience in bioinformatics and our researchers incorporate tools to mine data, call significantly DEG, link glycomics profiling with genomics besides provide comprehensive deliverables.
• What types of biological samples can be analyzed using CD BioGlyco's hybridization-based transcript analysis service?
  • Tissue samples: We analyze RNA extracted from various tissues, including the brain, liver, kidney, and more, to study tissue-specific gene expression profiles.
  • Cell lines: RNA from cultured cell lines can be used to investigate gene expression changes under different experimental conditions, such as drug treatments or genetic modifications.
  • Blood and body fluids: We process RNA from blood, plasma, serum, and other body fluids, enabling the study of circulating biomarkers and gene expression in systemic conditions.
  • Single cells: Our advanced techniques allow for the analysis of RNA from single cells, providing insights into cellular heterogeneity and individual cell responses.
  • Microbial samples: We specialize in analyzing RNA from microbial samples, including bacteria and fungi, to study pathogen-host interactions and microbial gene expression.

At CD BioGlyco, our hybridization-based transcript analysis service offers researchers an unprecedented high precision and depth tool for gene expression and sugar chain synthetic pathway exploration. By powering groundbreaking research and enabling the discovery of new biomarkers and therapeutic targets our service helps to drive progress in many biomedical areas. Please feel free to contact us to get more details about our Glyco-transcriptomics Transcript Analysis and we would like to help you with our wonderful services!

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• Amplification-based Transcript Analysis Service