Unveiling Glycan Secrets on Every Glycoprotein

CD BioGlyco has a long-term research interest in glycomics, and in conjunction with advanced glycoinformatics technology offers Glycomics Analysis Services in a variety of situations, such as Disease Glycomics Analysis. The analysis of glycans on specific glycoproteins is an important tool for the discovery of biomarkers for cancer diagnosis.

Here, with our first-class glycoinformatics technology, we provide our clients with analysis services of glycans on specific glycoproteins to help researchers conduct in-depth Cancer Glycomics Analysis and to promote the understanding of the process and function of protein glycosylation during cancer development.

Selective isolation and enrichment of glycoproteins

Selective isolation and enrichment of specific glycoproteins is often required before glycans on glycoproteins are analyzed. We offer a variety of methods to isolate and enrich specific glycoproteins.

Hydrophilic interaction liquid chromatography (HILIC): Separation and enrichment of specific glycoproteins is achieved by the difference in polarity between glycosylated and non-glycosylated peptides. Both preserve the structure of intact glycoproteins and can be used to enrich all types of intact glycoproteins.

Metal ion affinity chromatography (MIAC): We use this method to achieve selective enrichment of glycoproteins through the interaction between metal oxides or metal ions and glycoproteins. In addition, the method utilizes the differences in electrostatic properties and hydrophilicity between different glycoproteins.

Lectin affinity chromatography (LAC): Glycoprotein isolation and enrichment of intact glycoproteins is achieved by selective recognition of monosaccharides or glycans with a specific structure by a solid-loaded lectin. Depending on the specificity of the lectin, this method is widely used for the enrichment of specific N- and O-glycoproteins.

Mass spectrometry (MS)-based analysis of glycans

MS is an effective technique for analyzing glycans on specific glycoproteins. In cancer glycomics analysis, intact glycoproteins are separated and enriched into a high-resolution mass spectrometer to obtain spectra containing abundant fragment ions, and the analysis of glycans on glycoproteins is subsequently realized by spectral resolution techniques. Here, the MS techniques we provide for glycans analysis include but are not limited to the following.

Matrix-assisted laser desorption ionization MS (MALDI-MS): This is an MS technique that effectively analyzes N-glycans simply and rapidly. Since cancer development is accompanied by aberrant glycosylation modifications, and this aberrant glycosylation is manifested to some extent in changes in glycan abundance on glycoproteins, glycan changes can be effectively detected using MALDI-MS.

Electrospray ionization MS (ESI-MS): This method enables online separation of complex biological samples, reduces sample complexity, and provides efficient detection and analysis of glycans on glycoproteins for quantitative analysis of glycans.

Liquid chromatography- MS (LC-MS): This method obtains more abundant characteristic fragment ions of intact glycoproteins, enables the identification of the composition and sites of intact glycoproteins, and allows in-depth analysis of the structure of the glycans on specific glycoproteins and information on glycosylation sites.

Spectral analysis of glycans

After obtaining the mass spectra of glycans on specific glycoproteins, we analyze the glycans by matching them to a glycan database to qualitatively and quantitatively analyze the glycans.

First, we add the information on glycan structures that may be included in the process of cancer development in the database, and then we match the mass spectral information of glycans on glycoproteins with the database and use algorithmic techniques to infer the quality of glycan chains to realize the analysis of glycans. Meanwhile, in the process of glycan analysis, we provide matching with the proteomic database to identify the sequence of glycoproteins to enhance the accuracy of glycan analysis.

Publication Data

Applications

• Analysis of glycans on specific glycoproteins not only deepens the understanding of the protein glycosylation process and its function but also be used for the identification and development of novel diagnostic markers and therapeutic targets.
• Glycoproteins are involved in a variety of physiological activities in the body, and the analysis of glycans on specific glycoproteins can be used to help researchers explore the signaling processes between glycoproteins and host cells, glycans and cells, and glycans and proteins.
• Glycans on specific glycoproteins can be used to assess the heterogeneity of biopharmaceutical glycosylation and help researchers design and develop novel drugs.

Advantages

• We are equipped with advanced MS, chromatography, and bioinformatics technologies to accurately analyze and identify glycans on specific glycoproteins and to help clients analyze the sequence of glycoproteins and glycan glycoforms.
• Our professional team covers experts in molecular biology, chemistry, informatics, and other disciplines, and provides efficient and practical solutions for analyzing glycans on specific glycoproteins by analyzing complex data.
• We provide custom glycan analysis services based on client needs to ensure that we meet the needs of specific research.

Frequently Asked Questions

• What preparation is required before analyzing glycans on glycoproteins?
  • Analysis of glycans on specific glycoproteins usually requires high-purity glycoprotein samples, and protein concentration is critical for accurate analytical results. Therefore, before glycan analysis, glycoproteins need to be isolated and enriched. In some cases, the glycoproteins may also need to be deglycosylated.
• What is included in the analysis report that provides after we have analyzed a glycan?
  • The analysis report typically includes the type of glycan, structural characteristics, relative or absolute quantitative data, graphical analysis, and interpretation of results. In addition, we also provide relevant raw data for reference.

CD BioGlyco has an informatics system dedicated to glycomics research, providing custom analysis of glycans on specific glycoproteins to meet the needs of different clients. We provide our clients with one-stop glycan analysis services in the whole process, including sample processing, glycan analysis, and data analysis and reporting. Please feel free to contact us at any time and we will answer your questions promptly.

• Comparative Glycoproteomics of Serum
• Analysis of Glycoprotein Expression Changes in Cancer
• Analysis of Glycosylation Changes in Cancer
• Analysis of O-Linked Glycans in Cancer
• Comparative Glycoproteomic Analysis in Cancer
• Detection of Glycan Variation on Glycoprotein Markers