Analysis of Glycoprotein Expression Changes in Cancer
Glycoprotein Insights: Unmasking Cancer's Sugar Coating
Abnormal expression of glycoproteins on the cell surface triggers malignant transformation of the cell, and thus it is an important marker of cancer development. CD BioGlyco has accumulated deep experience in the field of
Disease Glycomics Analysis, and we utilize the glycoinformatics approach to perform a comprehensive
Analysis of Cancer Glycomics and to track the changes in the expression of glycoproteins in cancer.
Construction of the database
We obtain mass spectrometry (MS) data for glycoproteins from existing cancer-related glycoprotein expression profiling datasets, which typically contain samples from normal controls and cancer patients. Then, we use standardized algorithms to organize these data, such as cleaning incomplete or duplicate data, to complete the construction of the database.
In addition, we construct several databases with different functions according to the different research purposes of our clients and utilize these databases to study the changes in glycoproteins in cancer.
Expression analysis of glycoproteins
Data on the expression of specific proteins in healthy and malignant tissues is obtained from the database. This data shows the expression level of the glycoprotein and is compared between samples from the cancer entities shown. All datasets provide paired data for the class of glycoproteins.
We use computer simulations to research the relative differences in glycoprotein expression between healthy and cancer tissues. In this process, complete datasets of glycoprotein expression levels are extracted from the glycoprotein gene expression database. At the same time, each dataset provides paired samples collected from cancer and cancer-adjacent healthy tissues. To accurately analyze the overall expression of the entire glycoprotein, the expression values of all glycoproteins in each sample are added together for overall analysis.
In this process, we also use precise algorithmic techniques to analyze the changes that occur in different glycoproteins in cancer, such as the way glycans are linked and changes in glycan structure.
To assess whether the expression is reflected in the actual glycoprotein content, we analyze individual glycoprotein levels in each cell line by immunoblotting cell lysates.
Publication Data
Technology: Western blotting
Journal: BMC Cancer
IF: 3.4
Published: 2022
Results: In this article, the authors analyzed the expression of Annexin family members from different cancer cell lines. The presentation of Annexin on the cell surface during apoptosis in cancer cell lines was researched using western blotting. The authors compared Annexin levels in cancerous and healthy tissues by analyzing expression data from the Gene Expression Omnibus (GEO) database. The results showed that in all cancer cell lines, all expressed Annexins were transferred to the cell surface after induction of apoptosis. At least six Annexins could be consistently detected at both mRNA and protein levels, with AnxA1, AnxA2, and AnxA5 constituting the major part of Annexin expression.
Fig.1 Comparison of Anx expression between healthy and cancerous tissues. (Hein, et al., 2022)
Applications
- The expression of certain glycoproteins is significantly elevated in specific cancer types, so analysis of changes in glycoprotein expression can be used to identify biomarkers for cancer.
- Glycoprotein expression levels are associated with cancer aggressiveness and prognosis. By analyzing the expression changes of glycoproteins, it can be used to predict the progression of the disease, thus providing an important basis for clinical decision-making.
- Glycoproteins play a key role in cancer cells, and by analyzing the expression changes of glycoproteins, they can be used to screen out suitable targets for the development of novel therapeutic strategies.
Advantages
- We are equipped with an advanced
Glycomics Analysis platform combined with cutting-edge informatics technology to rapidly and accurately detect the expression status of glycoproteins in cancer.
- Our experienced researchers are capable of analyzing and interpreting complex glycoprotein expression-related data, and provide clients with professional reports on changes in glycoprotein expression in cancer.
- We provide one-stop solutions from sample preparation of glycoproteins, and analysis of glycoprotein expression data to interpretation of results to meet the specific research needs of our clients.
Frequently Asked Questions
- Why glycoprotein expression changes are important in cancer research?
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Glycoproteins play important roles in cell communication, adhesion, immune response, and other biological processes. Meanwhile, aberrant expression of glycoproteins in cancer cells may be associated with the onset, progression, and spread of cancer. Certain cancer types lead to significant changes in the expression of specific glycoproteins, which can serve as important biomarkers for early diagnosis and monitoring of disease progression. For example, prostate-specific antigen (PSA) and carcinoembryonic antigen (CEA) are widely used glycoprotein markers.
- What types of cancers are amenable to glycoprotein expression analysis?
- This type of analysis can be performed for almost all types of cancer, including but not limited to breast, bowel, lung, and prostate.
CD BioGlyco is committed to providing excellent services in analyzing the expression changes of glycoproteins in cancer to clients from a wide range of industries. Based on the specific needs of our clients, we provide personalized research solutions to ensure that the data and results meet or exceed their expectations. Please feel free to contact us if you have any questions.
Reference
- Hein, T.; et al. Molecular analysis of Annexin expression in cancer. BMC Cancer. 2022, 22: 994.
For research use only. Not intended for any diagnostic use.
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