Analysis of O-Linked Glycans in Cancer
Glycoinformatics Aids Precision in O-linked Glycan Analysis in Cancer
At CD BioGlyco, we are deeply involved in the field of glycomics and provide
Glycoinformatics-assisted Glycomics Analysis Services, which is a comprehensive service that integrates glycan chain analysis and information mining. Under this service framework, we further refine the scope of services and establish
Glycoinformatics-assisted Disease Glycomics Analysis Services, which focus on the analysis of disease-related glycan chain changes to reveal the unique role of glycosylation in the disease process. At the forefront of this service branch,
Glycoinformatics-assisted Cancer Glycomics Analysis Services is our highlight, which focuses on the in-depth analysis of cancer glycomics.
Aberrant O-linked glycosylation is a hallmark of many cancers and can affect tumor progression, metastasis, and immune evasion. Therefore, analyzing O-linked glycans is pivotal for understanding cancer biology.
Sample preparation
Our protocols are continuously optimized to ensure sample integrity and purity, accommodating a wide range of biological materials such as cell lines, tissue biopsies, and serum samples.
Protein extraction: We lyse cells or tissues to extract glycoproteins.
Protein enrichment and Isolation: We use affinity chromatography and precipitation methods to isolate glycoproteins.
Proteolytic digestion: We perform enzymatic digestion of proteins to release O-linked glycans.
Glycoengineering and enrichment
To ensure precise and detailed glycan analysis, we employ advanced glycoengineering techniques.
Enzymatic treatment: Specific glycosidases are used to release O-linked glycans.
Glycan labeling: We label glycans with fluorescent or mass tags to enhance detection sensitivity and accuracy.
Enrichment and purification: We use lectin affinity chromatography and solid phase extraction to enrich and purify O-linked glycans.
High-throughput glycan analysis
Our advanced analytical techniques allow for comprehensive glycan analysis.
Mass spectrometry (MS): High-resolution MS provides a detailed mass profile of glycans, while Tandem MS (MS/MS) provides structural information.
Liquid chromatography (LC): We use LC in combination with MS for the analysis of complex glycan mixtures.
Capillary electrophoresis (CE): CE ensures fast and efficient glycan separation.
Bioinformatics analysis
Glycoinformatics integrates glycan data with bioinformatics tools to perform multidimensional analysis.
Data processing and analysis: We use custom software for peak detection, glycan identification, and structural annotation.
Database integration: We cross-reference glycan data with databases such as GlyTouCan and UniCarbKB for comprehensive glycan characterization.
Network analysis: We map glycan structures to biological pathways and networks to elucidate their roles in cancer biology.
Interpretation and reporting
Our services culminate in a thorough interpretation of the analyzed data.
Consultative support: Clients can have one-on-one consultations with our experts to discuss findings, answer questions, and plan the next steps for research.
Comprehensive reporting: Detailed reports include glycan profiles, structural information, and quantitative data, as well as high-quality visualizations such as glycan maps and pathway diagrams.
Publication Data
Journal: International Journal of Molecular Sciences
IF: 4.9
Published: 2020
Results: The authors discussed how cancer glycosylation modifications involve alterations in glycan length, typically resulting in shorter O-glycans and more branched N-glycans. They noted that these glycosylation changes are often associated with significant variations in glycan sialylation and pyrophosphorylation, which impact terminal epitopes. Additionally, the authors provided an overview of various bioinformatics resources available for analyzing O-glycans. The intent of their article was to encourage collaboration between glycoinformatics scientists and cancer biologists to leverage existing glycoinformatics tools for cancer research.
Fig.1 Flowchart of glycosylation research. (Muthu, et al., 2020)
Applications
- This analytical technique can be used to deeply analyze O-linked glycosylation modifications on cancer-related proteins. This modification affects the folding, stability, and function of proteins, which in turn affects the signal transduction and metabolic processes of cells.
- This analytical technique can be used to efficiently and accurately identify O-linked glycosylation sites of specific proteins in cancer cells with the help of mass spectrometry technology and glycoinformatics tools.
- This analytical technique can be used to analyze the complex structure of O-linked glycan chains in detail, including their glycoforms, branching patterns, and linkage types.
Advantages of Us
- Our service relies on advanced glycoinformatics technology to achieve accurate analysis of O-linked glycosylation modifications on cancer-related proteins.
- Our service integrates cutting-edge algorithms and computational tools to efficiently process massive mass spectrometry data and quickly identify and analyze the structural information of O-linked glycan chains.
- Our service is not limited to simple glycan chain structure identification, but also deeply explores the multi-dimensional association between glycosylation modification and protein function.
Frequently Asked Questions
- Compared with traditional methods, what are the advantages of glycoinformatics technology?
- The advantages of glycoinformatics technology are mainly reflected in the following aspects: First, it can automatically process and analyze a large amount of mass spectrometry data and improve research efficiency and accuracy. Second, through advanced algorithms and models, it can accurately predict and analyze complex glycan chain structures. Third, the technology can integrate multi-source data, conduct multi-dimensional information mining, and reveal the deep relationship between glycosylation modification and protein function. Finally, glycoinformatics technology is still developing and innovating and can continue to bring breakthroughs to the scientific research field.
- What specific information can your analysis service provide?
- Our services can provide including but not limited to the following specific information: accurate identification of O-linked glycosylation sites, detailed structures of glycan chains (such as glycan forms, branching patterns, and connection bond types), quantitative information on glycosylation modifications, and potential associations between these modifications and protein functions or cell states. We use advanced glycoinformatics tools and algorithms to extract valuable information from massive data and present it to customers in an intuitive and easy-to-understand way.
At CD BioGlyco, we aim to advance our understanding of cancer biology by providing a comprehensive analysis of O-linked glycans in cancer. Through innovation and expertise, we are committed to supporting researchers in combating cancer more effectively. Please feel free to contact us if you are interested in our analysis of O-linked glycans in cancer.
Reference
- Muthu, M.; et al. Insights into bioinformatic applications for glycosylation: instigating an awakening towards applying glycoinformatic resources for cancer diagnosis and therapy. International Journal of Molecular Sciences. 2020, 21(24): 9336.
For research use only. Not intended for any diagnostic use.
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