Precise Analysis Reveals the Mystery of Serum Glycoproteins

CD BioGlyco has mastered the world's leading biological and informatics and other technological tools to comprehensively analyze a wide range of Diseases Glycomics. Since changes in serum glycoproteins are correlated with the cancer process, tracking changes in serum glycoproteins can be used to monitor the onset and progression of cancer. Therefore, in Cancer Glycomics Analysis, we utilize glycoinformatics technology to help researchers explore cancer development and find promising cancer biomarkers by comparing glycoproteins in serum.

Serum glycoprotein enrichment

After we get the serum samples, the serum proteins are separated into two fractions, low-abundance and high-abundance proteins, by acetonitrile precipitation. Then, they are broken down and digested using trypsin and protein endonuclease, and the glycoproteins are efficiently enriched by hydrophilic interaction chromatography (HILIC) before they are used for subsequent analysis.

Serum glycoprotein analysis

Firstly, mass spectrometry (MS) data from serum glycoproteins are used to generate a serum glycoproteomics database that combines algorithms and analysis software to analyze and compare serum glycoproteins. At the same time, the database displays features similar to those commonly found in glycoproteomics and is compatible with most search engines.

Secondly, we use orthogonal performance tests to train and optimize the analysis software. For example, in the performance test, we analyze the specific glyco-spectra of serum cuprocyanin (CP), haptoglobin (HP), and immunoglobulin G1 (IgG1), which have been fully characterized, by analyzing the software and optimizing the software based on the accuracy of the results.

Then, we take the serum glycoprotein to be tested and analyze it by MS, enter its fragmentation characteristics in the algorithm software, and run it through a database for rapid data matching and analysis. Different types of glycoproteins in serum display different features. For example, N-linked and O-linked glycoproteins have different chromatographic retention times, glycan mass numbers, or parent ion m/z.

After the analysis of serum glycoproteins is completed, the pivot table tool provided in Excel is used to compare all tested serum glycoprotein parameters. The parameters include glycan composition, protein sequence, modification sites, and changes that occur in the same glycoprotein in different physiological environments, among others.

Publication Data

Applications

• By comparing glycoproteins in the serum of healthy individuals and cancer patients, specific changes can be identified, which in turn can be used in the discovery and research of new cancer markers.
• In an infected state, pathogens may cause specific changes in host serum glycoproteins. Therefore, comparing serum glycoproteomics helps in the early diagnosis of diseases in research.
• The effect of immune response can be assessed by comparing serum glycoproteins of the host before and after vaccination, which can be used in vaccine development.

Advantages

• We utilize bioinformatics-based software and databases for high-throughput, high-precision identification and quantitative analysis of serum glycoproteins.
• Our team integrates experts in the fields of chemistry, glycobiology, and bioinformatics, with expertise in multiple disciplines and rich experience in Glycomics Analysis, to ensure the high accuracy and reliability of serum glycoproteomics analysis results.
• We provide technical support and data interpretation services to our clients to help them better understand and apply serum glycoproteomics-related technologies.

Frequently Asked Questions

• What is "comparative glycoproteomics of serum"?
  • Serum glycoproteomics is the science of researching the types, structure, and function of glycoproteins in serum. Comparative serum glycoproteomics is an analytical technique that is used to compare the composition and differences of glycoproteins in different samples. It can help reveal changes in glycoproteins in disease states, drug reactions, and other conditions.
• What are the main technologies used for comparative serum glycoproteomics?
  • MS: For accurate determination of the molecular weight and structure of glycoproteins.
  • LC: For separation and purification of glycoproteins.
  • Protein blotting (Western Blot): For detection of specific glycoproteins.
  • Bioinformatics tools: For analyzing and interpreting experimental data.

CD BioGlyco has a team of experienced and specialized technicians to provide our clients with one-stop solutions for serum glycoproteins. We provide personalized consulting services to our clients and customize serum glycoprotein analysis solutions according to project requirements. Please feel free to contact us if you have any questions in the field of serum glycoprotein analysis, our client service team is always ready to answer your queries.

• Analysis of Glycans on Specific Glycoproteins
• Analysis of Glycoprotein Expression Changes in Cancer
• Analysis of Glycosylation Changes in Cancer
• Analysis of O-Linked Glycans in Cancer
• Comparative Glycoproteomic Analysis in Cancer
• Detection of Glycan Variation on Glycoprotein Markers