100 glycan microarray service provided by CD BioGlyco helps researchers determine the binding partners for 100 diverse glycans in serum, proteins, plasma, cell cultures, cell/tissue lysates, or other potential biological samples, which represent the range of glycosylations found on cell membranes. We look forward to providing you with efficient scientific services in your research related to glycan microarray analysis.

Introduction

Glycocalyx is an extracellular polymer coating surrounding many eukaryotic and prokaryotic cells, consisting of glycolipids, glycoproteins, proteoglycans and glycosaminoglycans, which play important roles in cell signaling, viral infection and immune processes. Therefore, the clarification of glycan-associated biomolecular interactions and their consequences is critical for fundamental biology research and biomedical applications. However, there are currently very limited assay tools available for glycobiology research.

CD BioGlyco has worked to develop glycan microarrays for the rapid analysis of glycan-related recognition events. Because they contain numerous glycans immobilized in a compact and ordered manner on a solid surface, glycan microarrays allow for multiple parallel analyses of glycan-protein interaction using only a small number of glycan samples. Microarray technology is now being used as an effective tool to elucidate the role of glycans and glycan-binding proteins in biological systems.

Fig.1 Glycoconjugates on mammalian cell membranes. (Li, et al., 2021)

Our Services

CD BioGlyco provides customers with microarray analysis of 100 synthetic glycans. The most common identified structures displaying important binding functions in the literature are included, such as β-Glc-sp, β-Gal-sp, α-Man-sp, β-GlcNAc-sp, Neu5Ac-α-2,3-Gal-β-1,3-GlcNAc-β-sp, Gal-α-1,4-Gal-β-1,4-Glc-β-sp, GlcNAc-β-1,6-GlcNAc-β-sp, Neu5Gc-α-2,3-Gal-β-1,3-(Fuc-α-1,4)-GlcNAc-β-sp [Sialyl Lewis A], GlcNAc-β-1,6-(Gal-β-1,3)-GalNAc-α-O-Ser, GlcNAc-β-1,4-GlcNAc-β-sp, Gal-β-1,3-GalNAc-β-sp, Gal-α-1,3-(Fuc-α-1,2)-Gal-β-1,4-Glc-β-sp [Blood B antigen tetrasaccharide], GalNAc-β-1,4-(Neu5Ac-α-2,8-Neu5Ac-α-2,3)-Gal-β-1,4-Glc-β-sp [GD2], Neu5Ac-α-2,8-Neu5Ac-α-2,6-Gal-β-1,4-Glc-sp, etc. For more information about glycans, please contact us directly.

We provide 100 glycan microarray services to help researchers:

• Determination of glycans binding partners in various biological samples
• Rapid profiling of glycan-binding properties of proteins
• Evaluation of substrate specificities of several glycosyltransferases
• Screening for glycans that promote cell surface lectin-related cellular responses
• Study on inflammatory immune responses

Workflow

• 100 glycan microarray construction: We prepare various glycans through chemical synthesis or enzymatic synthesis. We then select a suitable functionalized glass slide surface and couple the synthesized glycans with linkers compatible with the printing surface, and print the glycan conjugates onto the glass slides.
• Sample preparation and labeling: We carefully process various samples according to established protocols and label them with fluorescent tags or other detectable labels.
• Microarray incubation and washing: Under precisely controlled conditions, the labeled target molecules are carefully incubated with the 100 glycan microarray. Non-specific binding materials are removed through washing. The microarray is scanned, and the bound materials are measured using a microarray scanner. The generated images can then be analyzed using various methods to produce microarray results. Our advanced equipment ensures accurate and sensitive detection of binding interactions.
• Data analysis: We use advanced software and algorithms to process raw data, provide detailed interpretations, and generate comprehensive reports. We also address any questions that may arise afterward.

Advantages

• The microarray format is designed to simultaneously analyze the interactions between the target molecule and 100 different glycans. This significantly accelerates the research process compared to traditional methods, which analyze one glycan at a time.
• Our glycan microarrays include a wide range of common and biologically relevant glycan structures, helping researchers gain a comprehensive understanding of the binding specificity of the target molecule.
• Glycan microarray assays are performed under controlled conditions with highly reproducible and reliable results.

Applications

• The 100 glycan microarray is used to discover and screen novel glycan-binding proteins in various biological samples and to determine their glycan-binding specificity.
• The 100 glycan microarray is used to understand glycan-protein interactions to analyze various biological processes, disease mechanisms, potential therapeutic targets, and more.
• The 100 glycan microarray is used to screen drug candidates for interactions with specific glycans.

Publication Data

Frequently Asked Questions

Equipped with high throughput and automated facilities, CD BioGlyco has developed glycans array analysis platform for screening protein-carbohydrate interactions. Our high-efficient systems help customers reduce project costs and timelines to accelerate their research. If you need our scientific services, please contact us for further consultation.

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