The pentose phosphate pathway consists of two branches. The oxidative branch has three irreversible reactions producing NADPH and ribonucleotides. The non-oxidative branch consists of a series of reversible reactions that recruit glycolytic metabolic intermediates. A growing number of studies have shown a strong link between the pentose phosphate pathway and the malignant progression of cancer cells. The activity of pentose phosphate pathway-related enzymes regulates the rate of the pentose phosphate pathway. Therefore, it is important to understand the activities of pentose phosphate pathway-related enzymes and how they are regulated. CD BioGlyco can provide the expression analysis, activity analysis, and regulatory mechanism analysis of pentose phosphate pathway-related enzymes based on proteomics technology.

Fig. 1 Schematic representation of pentose phosphate pathway (Cai Y, et al., 2019)

What We Do

Based on the MOPCGM platform, we can provide the following services.

• Expression analysis of the PPP related enzymes

We can provide the following PPP related enzymes expression assays.

• Glucose-6-Phosphate Dehydrogenase
• 6-Phosphogluconolactonase
• 6-Phosphogluconate Dehydrogenase
• Ribulose-5-phosphate Isomerase
• Transketolase and Transaldolase

• Analysis of post-translational modifications of PPP related enzymes

Different types of post-translational modifications in the pentose phosphate pathway regulate the activities of metabolic enzymes, provide macromolecular raw materials for the growth and proliferation of cancer cells, and affect the proliferation of cancer cells.

We can provide the following post-translational modifications of PPP related enzymes, including but not limited to:

• PPP related enzyme glycosylation assay
• PPP related enzyme phosphorylation detection
• PPP related enzyme acetylation assay
• Detection of post-translational modification sites of PPP related enzymes

• Interaction analysis of the PPP related enzymes

PPP related enzymes can form complexes with other proteins and regulate the functions of these proteins through metabolites.

We can provide the following interaction analysis of the PPP related enzymes, including but not limited to:

• Screening of proteins that interact with PPP related enzymes
• Prediction of the interaction sites of PPP related enzymes and proteins
• Analysis of the regulatory relationship between PPP related enzymes and proteins

Applications

Our services can support the following applications, including but not limited to:

• To explore the function of the pentose phosphate pathway in cancer stem cells' resistance to chemotherapy.
• To explore the differences in the expression of pentose phosphate pathway-related proteins in different molecular subtypes of cancer, and to determine the biological and clinical significance of the expression of pentose phosphate pathway-related proteins.
• To explore the mechanism of the pentose phosphate pathway in intracellular signaling mediated by exosomes.

Our Advantages

• We provide detailed glucose metabolism analysis services for the oxidative and non-oxidative branches of the pentose phosphate pathway, respectively.
• We provide the most comprehensive acylation analysis service for enzymes associated with the pentose phosphate pathway.

CD BioGlyco provides analysis services for the expression, post-translational modification, activity, and interaction mechanism of the pentose phosphate pathway related enzymes. Our experienced teams of scientists, researchers, and technicians provide fast turnaround, high-quality services at competitive prices for worldwide customers. Our customers have direct access to our staff and prompt feedback on their inquiries. If you are interested in our services, please contact us for more details.

Reference:

1. Farhadi P, et al. The emerging role of targeting cancer metabolism for cancer therapy. Tumour Biol. 2020 Oct; 42 (10): 1010428320965284.