Mesoporous materials have been widely used for glycopeptide enrichment because of their relatively large specific surface area, strong stability, and abundance of activation sites. CD BioGlyco has developed strategies for glycopeptide isolation and enrichment based on mesoporous materials. We have the confidence to provide customers with scientific assistance in the field of glycoproteomics.

Mesoporous Materials

The isolation and separation of glycopeptides using mesoporous materials have been reported in many glycoproteomics studies. Currently, several types of porous materials exist and are classified into different groups based on size. Macroporous and microporous materials have pore diameters greater than 50 nm and less than 2 nm, respectively, mesoporous materials are in the middle. Mesoporous silica nanomaterials show great promise in selectively capturing small-size targets from complex samples due to their regular pore arrangement, strong stability and easily functionalized inwalls. They are widely used in drug delivery, imaging, biosensors and other fields. Under the impact of strong capillary forces, bio-molecules entering mesoporous pores encounter more binding activation sites, thus enhancing the separation efficiency.

Advantages such as large specific surface area, size exclusion for large molecular weight biomolecules, surface modification potential, and support of strong capillary force at the nanoscale are the reasons for the widespread use of mesoporous materials. With their extensive applicability and adaptability, the use of mesoporous materials will have a sustained impact on the development of glycoproteomics.

Our Strategies

CD BioGlyco has established several effective solutions for the enrichment process of glycopeptides to increase the concentration of glycopeptides while removing other constituents. The strategies we offer include, but are not limited to:

• Mesoporous silica materials functionalized with boronic acid for the detection of glycopeptides. In this method, glycopeptides in solution bind with high affinity to the groups attached in the pores, significantly improving the solute detection limit.
• Enriched N-linked glycans by oxidized and ordered mesoporous carbon materials of CMK-3 followed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS, which allows the profiling of N-linked glycans in complex samples.
• Iminodiacetic acid (IDA) groups are grafted onto the inwalls of magnetic mesoporous silica (mSiO₂) nanomaterials for highly specific enrichment of N-linked glycopeptides.

Mesoporous Material-based Professional Glycopeptide Enrichment Service

• Highly Specific Enrichment

We use boric acid-functionalized mesoporous materials (such as mesoporous silica, mesoporous graphene-silica composites, etc.) for the specific enrichment of glycopeptides. These materials form reversible borate ester bonds with the cis-diol structures on glycans, enabling highly selective capture of N-glycopeptides and O-glycopeptides.

• Multifunctional Material Design

We not only use single-functional mesoporous materials, but also combine multiple functional groups (such as boric acid, zwitterionic groups, hydrophilic groups, etc.) to design multifunctional materials, thereby enhancing the enrichment efficiency and selectivity of glycopeptides. For example, our company combines boric acid-functionalized mesoporous silica with magnetic nanoparticles to achieve efficient enrichment and magnetic separation of glycopeptides.

• Glycopeptide Analysis

The enrichment method used by our company can be combined with a variety of MS techniques, including MALDI-TOF/TOF MS and nano liquid chromatography-tandem mass spectrometry (Nano-LC-MS/MS), to achieve high-precision identification of glycopeptides.

Workflow

Applications

• Our services are essential for characterizing the glycosylation of biotherapeutics, ensuring product quality, efficacy, and biosimilarity.
• Our services support detailed investigations into the roles of glycosylation in cell signaling, development, immunity, and host-pathogen interactions.
• Our services are not only applicable to glycoproteinomics research but also to multiple fields such as disease biomarker discovery, drug development, and metabolomics analysis.

Advantages of Us

• Our method delivers superior enrichment efficiency, demonstrating a significant enhancement in glycopeptide signal-to-noise ratios, often enabling the detection of glycopeptides present at concentrations far below the limits of conventional methods.
• The highly ordered porous structure of our materials ensures remarkable reproducibility and minimizes sample loss, contributing to more reliable and actionable data.
• We identify more types of glycopeptides from complex biological samples, significantly deepening the coverage of glycoproteomics.

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Frequently Asked Questions

CD BioGlyco has established friendly partnerships with many world-renowned enterprises, universities, and scientific research institutions, and provided them with high-quality services in the field of glycomics. Our services have been unanimously recognized by our customers. If you need scientific assistance, please contact us for further consultation.

Associated Services

To complement our mesoporous materials for glycopeptide enrichment, CD BioGlyco offers additional services that provide a more complete understanding of your glycosylation research.