Glycoprotein Enrichment
Protein glycosylation is ubiquitous and important in biological systems. Among hundreds of known modifications, glycosylation is one of the most common and necessary modifications. It determines protein folding, transportation and stability, and regulates almost all cell activities, including gene expression, signal transduction, extracellular activities and other responses. Glycoproteomics will remain an attractive research area because of the realization that glycosylation may be involved in the progress of many diseases. Therefore, the development of effective analytical tools to detect abnormal glycoprotein is very important for the in-depth understanding of the pathological function of glycoproteins and the development of new biomarkers. It is difficult to systematically and comprehensively analyze glycosylation modifications with conventional biochemical methods, but mass spectrometry in recent years has provided researchers with unique and site-specific opportunities to characterize protein modifications.
However, glycoproteins are not abundant compared to unmodified proteins. The heterogeneity of its glycan structure and the complexity of the various amino acid residues covalently bound to glycans make it still a great challenge to comprehensive analysis. Therefore, effective enrichment of glycopeptides /glycoproteins is essential before using MS for systematical analysis. Enrichment allows us to minimize the interference of high-abundance non-glycoproteins on glycosylation analysis and obtain enriched glycoproteins. CD BioGlyco provides a variety of advanced methods to enrich glycopeptides/glycoproteins.
CD BioGlyco, as a cutting-edge company with excellent expertise and rich experience in glycobiology research and services, is committed to providing rapid and efficient glycoprotein enrichment services to global researchers and related companies with its advanced technology platform, exquisite process, and senior professional team.
O-Glycoprotein and Peptide Enrichment
At CD BioGlyco, we offer a comprehensive glycoprotein enrichment strategy that utilizes high-affinity enrichment and purification of O-glycan-containing proteins and peptides with specific glycosidases, followed by quantification of O-glycoproteins and peptides in samples using highly sensitive and selective MS techniques.
Technology: MS, Lectin-based affinity enrichment, Gel electrophoresis
Journal: Molecules
Published: 2023
IF: 4.2
Results: In this article, 26 N-glycosylation sites of 11 N-glycoproteins were successfully enriched and identified from human tear fluid by a lectin-based affinity enrichment method combined with high-resolution MS. These glycoproteins, such as lactotransferrin and immunoglobulin heavy chain constant region α-1, were found to be associated with the pathogenesis of various chronic eye diseases (e.g., dry eye), which provides an important reference for future studies of tear N-glycoproteins as biomarkers or drug targets.
Fig.1 Sample preparation protocol for affinity enrichment of glycoproteins/glycopeptides. (Schmelter, et al., 2023)
CD BioGlyco has many advanced technologies for glycoprotein purification, we guarantee to provide the target glycoprotein that has high purity and high biological activity. Our researchers can also customize the experimental program for you according to your research needs.
Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.
Reference
