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LiGA-based Glycan-binding Profiling

Accurate Analysis Decoding LiGA-based Glycan-binding Profiling for You

CD BioGlyco has developed the Liquid Glycan Display Array (LiGA) using DNA barcoding, deep sequencing, and glycan coupling to generate phage virus particles. Thus, we provide high-quality analysis and identification services of LiGA-based glycan-binding in vivo, in vitro, and on the cell surface. Based on the Glycan Display Platform, we provide complete LiGA services including LiGA Construction, Glycan Modification, Quality Control, Functional Validation, and profiling services. Our goal is to help our client overcome challenges on the path to the biological functions of polysaccharides. At CD BioGlyco, our research team provides LiGA development services with varying amounts of glycoconjugates. Meanwhile, our service enables part of the glycan display at different densities. We provide a comprehensive analytical service of LiGA-based glycan-binding.

Differential enrichment (DE) analysis service

To identify significantly enriched glycans, we detect phage DNA sequences by DE analysis service. Meanwhile, we trim the m-value mean (TMM) normalization using a negative binomial model, Benjamini-Hochberg (BH) correction to control the false discovery rate (FDR) in a specific range.

Next-generation sequencing (NGS) analysis service

We use NGS to analyze glycan-glycan binding protein (GBP) interactions. Our researchers rapidly and accurately analyze specific GBP's glycan-pathogen interactions by deep sequencing services. This analysis report contains key information for the rapid recognition of glycans with GBP. Moreover, we provide deep sequencing services for cell surface phages to resolve the relevant binding interactions.

Validation and extension services

We provide difference information analysis services between LiGA-glycoconjugate and Traditional Glycan Display Array and contrast the binding information for each locus. Furthermore, we provide analytical services for the combination of modified GBP (such as biotinylated) and LiGA.

Fig.1 Schematic diagram of LiGA-based glycan-binding profiling service. (CD BioGlyco)

Publication

Paper Title: Genetically encoded multivalent liquid glycan array displayed on M13 bacteriophage

Technology: DE analysis, Microarray

Journal: Nature Chemical Biology

Published: 2021

IF: 16.284

Results: In this article, LiGA-75 containing 75 glycoconjugates was used. 15 glycans significantly associated with galectin-3 (G3C)-sa beads rather than sa beads alone were successfully identified by DE analysis methods, negative binomial modeling, TMM normalization, and FDR calculations. The results of glycan analysis using LiGA-75 were consistent with those obtained using slide-printed microarrays based on the database. In addition, the binding of biotinylated GBP and LiGA-65 antibodies was analyzed. The results showed general agreement between glycan-GBP interactions measured by LiGAs and the binding of lectins to printed glycan microarrays. Notably, some of the binding exhibited in the LiGAs was not found in the printed arrays.

Fig.2 The multi-analysis data atlas. (Sojitra, et al., 2021)

Applications of LiGA-based Glycan-binding Profiling

LiGA-based glycan-binding profiling provides critical information for deciphering glycan-binding information in vivo and in vitro.
LiGA-based glycan-binding profiling can be used to detect GBP on the cell surface in a natural environment.
LiGA-based glycan-binding profiling is a new target for the development of diagnostic and therapeutic applications.

Advantages of Us

Our operators display specific glycoconjugates at specific densities at clients' request.
When interrogating these glycan-GBP interaction modes, our operators overcome the challenge of the number of binding sites and the spatial presentation of the glycans.

CD BioGlyco has worked on the development of glycan displays for many years. Our lab continues to improve the quality of LiGA service to meet the overall needs of our clients. We hope to accelerate the progress of your project. If you are interested in the LiGA, please feel free to contact us.

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