Diacylglycerol Analysis

Diacylglycerol Analysis

The diversity of lipid structure endows lipids with many important biological functions. As a neutral lipid, diacylglycerol (DAG) has the potential to improve bone health, inhibit fat accumulation in the body, and reduce postprandial serum triglyceride, cholesterol and glucose levels. At present, it has been widely used in the medical and food industries. CD BioGlyco offers high-quality DAG analysis services by liquid chromatography (LC) and nuclear magnetic resonance (NMR).

Roles of DAG

DAG, as a neutral lipid substance, is widely present in various tissue cells in animals. In the phospholipid bilayer structure of biological membranes, DAG forms an important precursor of many phospholipids. In complex lipid metabolism, DAG is an important intermediary product. In addition, DAG, as a precursor or mediator of a variety of hormones, also plays an important role in the regulation of hormone levels in the organism. With people’s research on cell signal transduction mechanisms, it has been discovered that DAG is still a second messenger substance. It uses external stimulus signals to activate an important protein kinase C (PKC) in the cell and phosphorylate various substrate proteins to produce corresponding biological effects.

Studies have shown that DAG has the potential to improve bone health, inhibit body fat accumulation, and reduce postprandial serum triglyceride, cholesterol and glucose levels. Due to its claimed health benefits, DAG has been widely used in the medicine and food industry. DAG has also received widespread attention as a low-calorie fat substitute and has been incorporated into various food matrices.

Sources of DAG in the cell.Fig.1 Sources of DAG in the cell. (Kolczynska, 2020)


With the wide application of DAG in the fields of medicine and food, the development of analytical methods for the content and structure of DAG is essential. At CD BioGlyco, we provide DAG analysis services including but not limited to:

  • Analysis of DAG molecules in cell lipid extracts by normal-phase liquid chromatography (LC)-electrospray mass spectrometry

    1) Solvent extraction of neutral lipids (ethyl acetate/isooctane); 2) 2,4-Difluorophenyl isocyanate derivatizes free hydroxyl groups into diacyl carbamates; 3) Normal phase high-performance liquid chromatography (HPLC) combined with positive ion electrospray ionization tandem mass spectrometry is used to separate regioisomeric DAG.

  • High-resolution NMR spectroscopy for qualitative and quantitative analysis of DAG oil

    High-resolution 1H- and 13C-NMR spectroscopy has been widely used to analyze complex mixtures. NMR spectroscopy is a universal, fast, and effective analysis method for DAG oil. It provides qualitative and quantitative information about various acylglycerols and acyl chains and other minor compounds during the manufacturing process of DAG oil.

Applications of DAG Analysis

  • Study the role of DAG in cell metabolism and proliferation
  • Detection of 1,3-diglycerides in milk fat
  • Quantitative analysis of DAG content in cells
  • Research on the function of DAG to reduce weight and blood lipid

Advantages of Us

  • Fast and effective analysis
  • Comprehensive analysis method
  • Customized experiment plan and complete experiment report
  • 24 hours after-sales service

With years of research on Lipid Analysis, CD BioGlyco have professional knowledge reserves and analytical experience. We are very happy to continue to explore the lipid field with you. If you have any needs in this regard, please feel free to contact us.


  1. Kolczynska, K.; et al. Diacylglycerol-evoked activation of PKC and PKD isoforms in regulation of glucose and lipid metabolism: a review. Lipids in Health and Disease. 2020, 19(1): 1-5.
  2. Leiker, T.J; et al. Analysis of diacylglycerol molecular species in cellular lipid extracts by normal-phase LC-electrospray mass spectrometry. International Journal of Mass Spectrometry. 2011, 305(2-3): 103-108.
This service is for Research Use Only, not intended for any clinical use.

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