Exosomes are the most important extracellular vesicles. As carriers, they mainly contain intracellular and extracellular proteins, coding and non-coding RNAs, DNA, and metabolites. In the tumor microenvironment, exosomes derived from cancer cells and various stromal cells can regulate the reprogramming of glucose metabolism in cancer cells and even act on other cells. Therefore, studying the function of exosomes in cancer glucose metabolism can provide necessary information for exosome engineering, cancer therapy, and real-time monitoring of exosomes in tumor prognosis. CD BioGlyco can provide proteomics, metabolomics, and genomics detection services related to exosome glucose metabolism to help customers analyze the "bridge" function of exosomes between cancer cells and the tumor microenvironment.

What We Do

• Exosome source analysis

In the tumor microenvironment, exosomes are derived from cancer cells and various stromal cells. Different sources of exosome receptor cells are different, and at the same time play different roles in the regulation of glucose metabolism. Analysis of the source of exosomes can lay the foundation for further research on the function of exosomes in cancer glucose metabolism.

• Exosome internalization analysis

Exosome internalization assays in order to identify recipient cells for exosomes in the tumor microenvironment. Since different cells have different uptake capabilities of exosomes, analyzing the internalization of exosomes can help us understand the uptake capabilities of exosomes in different cells and the regulation of exosomes on the glucose metabolism of each component by transmitting the information.

• Exosome content analysis

Exosomes contain a variety of biomolecules, including proteins, RNA, DNA, and metabolites. Exosomes in the tumor microenvironment can act as message transmitters, delivering biomolecules carried to nearby or distant recipient cells. Through the MOPCGM platform, we are able to quantitatively analyze the expression of enzymes related to glucose metabolism, the content of glucose metabolites, and the content of RNA with glucose metabolism regulation function in exosomes.

• Regulatory role analysis of exosomes in glucose metabolism

After exosomes are taken up by cancer cells, the signals transmitted by exosomes can regulate their glucose metabolism pathways, thereby promoting cancer cell growth. For example, cancer cells with enhanced resistance to glycolysis can secrete large amounts of exosomes containing glycolytic enzymes and stem cell-like markers, and adjacent cancer cells take up these exosomes, ultimately leading to the adjacent cancer cells' glycolytic pathway enhanced.

Deliverables

• Results of analysis of extracellular exosome transport pathways in the tumor microenvironment.
• Results of heterogeneity analysis of glucose metabolites contained in exosomes in the tumor microenvironment.
• Molecular mechanism diagram of exosomes regulating glucose metabolism in cancer cells in the tumor microenvironment.

Applications

Our services can support the following applications, including but not limited to:

• To explore the effect of glucose metabolism-related proteins contained in exosomes on the chemotherapy resistance of cancer cells.
• Research on exosomes as endogenous drug carriers to deliver small molecule cancer therapeutics.
• Analysis of the effects of glucose metabolism modulators on exosome content.
• Differential analysis between metabolic phenotypes of cancer cells and their internalized exosome contents.
• To explore the potential of exosome-encapsulated RNAs related to glucose metabolism regulation as cancer biomarkers.

CD BioGlyco provides exosome source analysis, exosome uptake analysis, exosome content analysis, and regulation analysis of exosomes on glucose metabolism pathways. Our experienced teams of scientists, researchers, and technicians provide fast turnaround, high-quality services at competitive prices for worldwide customers. Our customers have direct access to our staff and prompt feedback on their inquiries. If you are interested in our services, please contact us for more details.