Total Fatty Acid Analysis

Total Fatty Acid Analysis

Fatty acids (FAs) are the main components of lipids, CD BioGlyco has complete Fatty Acid Analysis solutions and experienced scientists to meet customers' total fatty acids (TFAs) analysis needs. Welcome customers all over the world to cooperate with us!


Fat is an indispensable nutrient and basic metabolite for our life, and it is the main form of energy storage in the body. Fat is composed of a complex mixture of FAs, including saturated, monounsaturated, and polyunsaturated compounds with various carbon chain lengths. Since the role of FAs in the body depends on the structure, it is necessary to conduct a detailed analysis of the composition of FAs.

FAs are the main components of lipids, and the physical, chemical, and physiological properties of lipids mainly depend on the composition of FAs. Generally, the composition of FAs is analyzed by gas chromatography (GC). Therefore, it is necessary to convert TFAs into derivatives with less polarity and higher volatility. After lipid extraction, TFAs are usually converted to their methyl ester (FAs methyl ester, FAME) before chromatographic analysis. Boron trifluoride methanol solution is the commonly used derivatization reagent for FAs methyl esterification. One of the key issues of analyzing FAME from TFAs is to choose a suitable chromatographic column to separate them, which mainly depends on the objective of the analysis, and the internal standard method is usually used for quantification.

Chromatographic analysis of TFAs in serum/plasma by gas chromatography-negative chemical ionization-mass spectrometry (GC-NCI-MS).Fig.1 Chromatographic analysis of TFAs in serum/plasma by gas chromatography-negative chemical ionization-mass spectrometry (GC-NCI-MS). (Kish-Trier, 2016)


FAs are one of the main energy sources of the human body. The FAs present in the sample are transesterified into the form of methyl esters, which are quantified by GC-based technology. By using different chromatographic parameters (including column materials and gradients) and detection principles, FAs analysis is monitored at different structural resolution levels.


Total Fatty Acid Analysis

Advantages of Us

  • Fast separation speed, good separation effect, and good selectivity
  • Reliable and repeatable method
  • The internal standard method for quantification


  • Determine the presence of FAs impurities
  • Determine the types of free fatty acids (FFAs) and TFAs of products
  • Prove the consistency between batches for release in the production process
  • Start preliminary research before doing a more detailed analysis of the lipids

CD BioGlyco is a globally recognized leader in FAs analysis, and there are many successful TFAs analysis cases. We use GC-based technology to develop a reliable and reproducible method to determine TFAs in different sample types. If you are interested in our services, please feel free to contact us.


  1. Kish-Trier, E.; et al. Quantitation of total fatty acids in plasma and serum by GC-NCI-MS. Clinical Mass Spectrometry. 2016, 2: 11-17.
This service is for Research Use Only, not intended for any clinical use.

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