Lipopolysaccharide Purification

Our rich experience in the purification and structural analysis of lipopolysaccharides and the advanced technology platform guarantee the quality of our services. We have confidence to be your essential research assistant in the field of glycobiology.

Background

Lipopolysaccharide (LPS) is the main outer membrane component of gram-negative bacteria, accounting for about 75% of the total surface weight. Its basic structure consists of lipid A, core oligosaccharides, and repeating polysaccharides called O-antigens. Lipid A is highly conserved and has endotoxin activity, while the O-antigen carbohydrate chain is a polymer of repetitive oligosaccharides, which vary between different species and are responsible for the serological specificity of bacteria. The lipid A part in LPS enters the lipid layer as a component of the outer lipid bilayer, and the sugar chain is exposed outside the cell. LPS has thermal and chemical stability and cannot be inactivated by a general autoclave or dry heat sterilization method.

LPS can cause pathophysiological effects such as fever, leukopenia, and leukocytosis. Because of its important pathogenic effect in diseases caused by gram-negative bacteria, many researchers have conducted studies on its isolation and purification. Contamination of proteins and nucleic acids is the main disadvantage of the LPS purification scheme. These contaminations will hinder the application of the final product in sensitive assays such as molecular and immunological experiments. Although ultracentrifugation can be used to eliminate contaminating proteins, this usually results in a decrease in yield.

Fig 1. Architecture of the Gram-negative cell envelope.Fig 1. Architecture of the Gram-negative cell envelope
A) Depiction of the Gram-negative cell envelope. B) Structure of prototypical LPS produced by E. coli. (Bertani, B.; Ruiz, N. 2018)

Services

CD BioGlyco has optimized the procedure based on hot phenol extraction to better purify LPS and make it more active. The purification process we provide includes but is not limited to the following steps.

  • Bacterial cell culture
  • LPS extraction and purification
  • Silver, coomassie blue and ethidium bromide staining
  • High performance liquid chromatography (HPLC) separation
  • Limulus amebocyte lysate (LAL) assay
  • Rabbit pyrogen test

Applications

  • Research on antibodies and inhibitors of lipopolysaccharide-related diseases (such as sepsis)
  • Structure and function research of lipopolysaccharide
  • Study on the protective mechanism of lipopolysaccharide on gram-negative bacteria
  • Composition research of lipopolysaccharide

Advantages of Us

  • High purity and less protein and nucleic acid pollution
  • Able to handle a variety of sample types
  • Optimized on traditional methods
  • Well-trained researchers

We constantly update our technology platform to provide customers with comprehensive and high-quality lipopolysaccharide purification and profiling services. We have provided successful services to scientific researchers from all over the world. CD BioGlyco will continue to raise the standard to meet customers' glycobiology research needs.

Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.

References:

  1. Bertani, B.; Ruiz, N. Function and biogenesis of lipopolysaccharides. EcoSal Plus. 2018, 8(1).
  2. Kilár, A.; et al. Structural characterization of bacterial lipopolysaccharides with mass spectrometry and on- and off-line separation techniques. Mass Spectrometry Reviews. 2012, 32(2): 90-117.
This service is for Research Use Only, not intended for any clinical use.

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About Us

CD BioGlyco is a world-class biotechnology company with offices in many countries. Our products and services provide a viable option to what is otherwise available.

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