Ion Exchange Chromatography-based Lectin Purification Service

Ion Exchange Chromatography-based Lectin Purification Service

Ion Exchange Chromatography-based Lectin Purification Service at CD BioGlyco

CD BioGlyco customizes the Lectin Purification services exclusively to meet the requirements of our clients. Here, efficient purification is achieved through ion exchange chromatography (IEC), an effective technique for separating and purifying lectins, as well as our expertise and experience in purifying lectins from plants, animals, etc.

  • How does IEC work?

IEC is a widely used chromatographic method for lectin purification. Its stationary phase is a chemically bonded ion exchanger or ion exchange resin. This method utilizes the difference in selectivity coefficients or ion exchange capacity of the components being separated. Different biomolecules have different charges and therefore they have different binding capacities to ion exchangers. When eluted, these substances are eluted off in a different order, thus achieving separation.

Fig.1 Principle of IEC. (Cummins, et al., 2017)Fig.1 Principle of IEC. (Cummins, et al., 2017)

  • We combine affinity chromatography on mannose-sepharose with IEC to obtain a highly pure preparation oflectins.
  • Some lectins are purified by a combination of ammonium sulfate precipitation, dialysis, IEC, and gel filtration chromatography.
  • In addition, CD BioGlyco offers GSL isolation and purification services based on IEC.
  • We not only provide lectin purification service based on IEC but also based on Gel-filtration Chromatography, Affinity Chromatography, and so on.

Fig.2 Flow chart of our purification services. (CD BioGlyco)Fig.2 Flow chart of our purification services. (CD BioGlyco)


Technology: Ion Exchange Chromatography

Journal: Acta Oceanologica Sinica

IF: 2.127

Published: 2018

Results: In this study, cation exchange chromatography coupled with gel filtration chromatography was used for the isolation and purification of lamprey (Lampetra japonica) serum. Elution fractions were collected based on hemagglutination activity, resulting in a polymeric lectin (105-kDa). The purification method was tested to be efficient and yielded a single fraction.

Fig.3. Ion exchange chromatography profile of lamprey serum crude extract on SP-Sepharose column. (Han, et al., 2018)Fig.3 Ion exchange chromatography profile of lamprey serum crude extract on SP-Sepharose column. (Han, et al., 2018)


  • IEC is successfully applied to analyze bulk active ingredients, excipients, raw materials, impurities, degradation products, counter ions, diluents, and different stages of the production process.
  • IEC is also used to analyze the compatibility of production waste streams, equipment cleaning fluids, and containers.
  • IEC is used to analyze cations and anions including oligosaccharides, aminoglycosides (antibiotics), monosaccharides, metal ions, alditols, other polyhydroxy compounds, nucleosides, nucleotides, and other polar molecules.
  • IEC analyzes a wide range of biomolecules in biotechnology, agriculture, pharmaceutical, environmental and other industries.


  • We supply high-purity compounds with the service of quick and high credibility.
  • The operation process is flexible and is adjusted according to actual needs.
  • All operations are completed in a closed system with less pollution.
  • High throughput, powerful resolving ability, high performance, large automation, and sample handling capacity.

CD BioGlyco depends on the objective and required purity to optimize IEC-based lectin purification methods. Please feel free to contact us if you need plant, fungal, or animal lectin purification services.


  1. Cummins, P.M.;et al. Ion-exchange chromatography: basic principles and application. Protein Chromatography: Methods and Protocols. 2017: 209-223.
  2. Han, Y.; et al. Target-directed isolation and identification of a serum lectin from lamprey (Lampetra japonica) by chromatographys and MALDI-TOF/TOF. Acta Oceanologica Sinica. 2018, 37: 113-116.
This service is for Research Use Only, not intended for any clinical use.

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