Ceramide Lipid Analysis Service

Ceramide Lipid Analysis Service

CD BioGlyco has developed a complete lipidomics analysis solution for clients. We provide fast, efficient, and accurate ceramide lipid analysis services by mass spectrometry (MS). Welcome global customers to establish cooperation with us!

Structure of Ceramide

Ceramide is a complex class of sphingolipids located at the center of sphingolipid synthesis and degradation metabolism. It is composed of saturated or unsaturated long-chain fatty acids of C14-C26 chain length and sphingosine long-chain bases. It mainly includes ceramide phosphocholine and ceramide phosphoethanolamine, which is a class of important biologically active substances. Ceramide is produced in the endoplasmic reticulum (ER) and then transported to the Golgi by ceramide transfer protein (CERT) or vesicles.

There are three main pathways of ceramide production: sphingomyelin hydrolysis, de novo synthesis, and salvage pathway. First, the sphingomyelin hydrolysis pathway uses sphingomyelinase to break down sphingomyelin in the cell membrane and release ceramides, which occurs in the plasma membrane, lysosomes, Golgi apparatus, and mitochondria. Second, the de novo synthesis of ceramides begins with the condensation of palmitate and serine to form 3-keto-dihydrosphingosine, which occurs in the ER. Third, in the salvage pathway, complex sphingolipids are converted to sphingosine and then generate ceramides by reacylation, which occurs in lysosomes and endosomes.

Fig.1 Sphingolipid metabolism pathway.Fig.1 Sphingolipid metabolism pathway. (Turpin-Nolan & Brüning, 2020)

Functions of Ceramides

Ceramide is an essential intermediate in the biosynthesis of all complex sphingolipids and is present in all eukaryotic cells. It also acts as an intracellular lipid second messenger and plays an important role in a variety of life activities, such as cell proliferation, differentiation, migration, adhesion, apoptosis, and aging. In addition, ceramides are also involved in many pathological processes, such as cardiovascular disease, cancer, inflammation, etc. In short, ceramide plays important roles in both physiological and pathological processes. Therefore, accurate quantification of ceramides in various samples is very necessary.

Ceramide Lipid Analysis Service

At CD BioGlyco, we have developed high-sensitivity, reproducible, high-resolution, and high-accuracy solutions for sphingolipid analysis. We provide ceramide, omega-hydroxyceramides (EOCer), non-hydroxyceramides (NCer), and alpha-hydroxyceramides (ACer) analysis in various samples by Q Exactive Orbitrap-MS. Ceramides we analyze include but are not limited to omega-hydroxy-dihydrosphingosines (EOdS), omega-hydroxy-sphingosines (EOS), omega-hydroxy-phytosphingosines (EOP), omega-hydroxy-6-hydroxysphingosines (EOH), non-hydroxy-dihydrosphingosines (NdS), non-hydroxy-sphingosines (NS), non-hydroxy-phytosphingosines (NP), non-hydroxy-6-hydroxysphingosines (NH), alpha-hydroxy-dihydrosphingosines (AdS), alpha-hydroxy-sphingosines (AS), alpha-hydroxy-phytosphingosines (AP), and alpha-hydroxy-6-hydroxysphingosines (AH).

Type Substance Method anges
Ceramide Ceramide Q Exactive Orbitrap-MS
  • Total length from 30 to 46
  • Total double bond from 0 to 2
  • Total hydroxyl group from 2 to 4
EOCer EOdS Q Exactive Orbitrap-MS
  • Total length from 62 to 74
EOS Q Exactive Orbitrap-MS
EOP Q Exactive Orbitrap-MS
EOH Q Exactive Orbitrap-MS
NCer NdS Q Exactive Orbitrap-MS
  • Long-chain base length from 14 to 34
  • Fatty acid length from 14 to 32
NS Q Exactive Orbitrap-MS
NP Q Exactive Orbitrap-MS
NH Q Exactive Orbitrap-MS
ACer AdS Q Exactive Orbitrap-MS
  • Long-chain base length from 14 to 34
  • Fatty acid length from 14 to 32
AS Q Exactive Orbitrap-MS
AP Q Exactive Orbitrap-MS
AH Q Exactive Orbitrap-MS
In addition, we also provide diverse ceramide products for global clients, including but not limited to:

Advantages of Us

  • Comprehensive solutions for lipidomics analysis
  • High-sensitivity and reproducible
  • High-resolution and high-accuracy
  • Experienced scientist

CD BioGlyco has been recognized by scientists from several countries. We are willing to share our knowledge and experience in this field. If you are interested in our services and products, please contact us for more details without any hesitation.


  1. Turpin-Nolan, S.M.; Brüning, J.C. The role of ceramides in metabolic disorders: when size and localization matters. Nature Reviews Endocrinology. 2020, 16(4): 224-233.
This service is for Research Use Only, not intended for any clinical use.

About Us

CD BioGlyco is a world-class biotechnology company with offices in many countries. Our products and services provide a viable option to what is otherwise available.

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