CD BioGlyco has developed a complete lipidomics analysis solution for clients. We provide fast, efficient, and accurate ceramide lipid analysis services by mass spectrometry (MS). Welcome global customers to establish cooperation with us!
Ceramide is a complex class of sphingolipids located at the center of sphingolipid synthesis and degradation metabolism. It is composed of saturated or unsaturated long-chain fatty acids of C14-C26 chain length and sphingosine long-chain bases. It mainly includes ceramide phosphocholine and ceramide phosphoethanolamine, which is a class of important biologically active substances. Ceramide is produced in the endoplasmic reticulum (ER) and then transported to the Golgi by ceramide transfer protein (CERT) or vesicles.
There are three main pathways of ceramide production: sphingomyelin hydrolysis, de novo synthesis, and salvage pathway. First, the sphingomyelin hydrolysis pathway uses sphingomyelinase to break down sphingomyelin in the cell membrane and release ceramides, which occurs in the plasma membrane, lysosomes, Golgi apparatus, and mitochondria. Second, the de novo synthesis of ceramides begins with the condensation of palmitate and serine to form 3-keto-dihydrosphingosine, which occurs in the ER. Third, in the salvage pathway, complex sphingolipids are converted to sphingosine and then generate ceramides by reacylation, which occurs in lysosomes and endosomes.
Fig.1 Sphingolipid metabolism pathway. (Turpin-Nolan & Brüning, 2020)
Ceramide is an essential intermediate in the biosynthesis of all complex sphingolipids and is present in all eukaryotic cells. It also acts as an intracellular lipid second messenger and plays an important role in a variety of life activities, such as cell proliferation, differentiation, migration, adhesion, apoptosis, and aging. In addition, ceramides are also involved in many pathological processes, such as cardiovascular disease, cancer, inflammation, etc. In short, ceramide plays important roles in both physiological and pathological processes. Therefore, accurate quantification of ceramides in various samples is very necessary.
At CD BioGlyco, we have developed high-sensitivity, reproducible, high-resolution, and high-accuracy solutions for sphingolipid analysis. We provide ceramide, omega-hydroxyceramides (EOCer), non-hydroxyceramides (NCer), and alpha-hydroxyceramides (ACer) analysis in various samples by Q Exactive Orbitrap-MS. Ceramides we analyze include but are not limited to omega-hydroxy-dihydrosphingosines (EOdS), omega-hydroxy-sphingosines (EOS), omega-hydroxy-phytosphingosines (EOP), omega-hydroxy-6-hydroxysphingosines (EOH), non-hydroxy-dihydrosphingosines (NdS), non-hydroxy-sphingosines (NS), non-hydroxy-phytosphingosines (NP), non-hydroxy-6-hydroxysphingosines (NH), alpha-hydroxy-dihydrosphingosines (AdS), alpha-hydroxy-sphingosines (AS), alpha-hydroxy-phytosphingosines (AP), and alpha-hydroxy-6-hydroxysphingosines (AH).
|Ceramide||Ceramide||Q Exactive Orbitrap-MS||
|EOCer||EOdS||Q Exactive Orbitrap-MS||
|EOS||Q Exactive Orbitrap-MS|
|EOP||Q Exactive Orbitrap-MS|
|EOH||Q Exactive Orbitrap-MS|
|NCer||NdS||Q Exactive Orbitrap-MS||
|NS||Q Exactive Orbitrap-MS|
|NP||Q Exactive Orbitrap-MS|
|NH||Q Exactive Orbitrap-MS|
|ACer||AdS||Q Exactive Orbitrap-MS||
|AS||Q Exactive Orbitrap-MS|
|AP||Q Exactive Orbitrap-MS|
|AH||Q Exactive Orbitrap-MS|
|Cat. No.||Product Name||Cat. No.||Product Name|
|XGB658||Sphingolipid ceramide N-deacylase from Pseudomonas sp.||XGB745||N-Octanoyl-D-sphingosine|
|XGB730||Ceramide phosphorylethanolamine||XGB746||Non-hydroxy fatty acid ceramide from bovine brain|
|XGB736||DL-Dihydrosphingosine||XGB752||D-Erythro-ceramide C8 1-phosphate|
|XGB737||L-Threo-dihydrosphingosine||XGB753||Ceramide 1-phosphate from bovine brain|
|XGB738||Sphingomyelin, ≥95%||XGB775||Phytosphingosine hydrochloride|
|XGB739||Sphingomyelin, ≥98.0% (TLC)||XGB981||Globotriaosylsphingosine from porcine blood|
|XGB741||D-Erythro-ceramide C8||XCM0418||β-D-Glucosyl C4-ceramide|
|XGB742||Ceramide from bovine spinal cord||XGO0001Q||N-Stearoyl-D-sphingomyelin|
CD BioGlyco has been recognized by scientists from several countries. We are willing to share our knowledge and experience in this field. If you are interested in our services and products, please contact us for more details without any hesitation.