Precision Gamma Cyclodextrin (γ-CD) Analysis for Maximum Efficiency

γ-CD is a cyclic oligosaccharide produced by the action of cyclodextrin glucosyltransferase on starch with eight glucose bound by α-1,4-glycosidic bonds. γ-CD has several special properties, the most notable of which is its ability to encapsulate non-polar compounds. Its cavity structure, which can hold non-polar molecules inside by encapsulation, improves their stability, solubility, and bioavailability. Thus, γ-CD has become a versatile pharmaceutical excipient.

As a professional glycobiology research company, CD BioGlyco has accumulated deep experience in the field of Pharmaceutical Excipients Analysis, providing clients with a comprehensive and reliable γ-CD analysis service.

• Identification
  • Compare the high performance liquid chromatograms of the sample and the γ-CD standard, if the positions of the main peaks in the graphs and the retention times are the same, then the sample is γ-CD.
  • Test the infrared spectrum of the sample and compare its spectrogram with the infrared spectrogram of the γ-CD standard. If the chemical shift values and intensities of the peaks in the spectrogram of the sample agree with those of the standard, this sample is γ-CD.
• Specific rotation
  γ-CD is dissolved in water and its specific rotation is measured using a spinometer. The specific rotation of γ-CD ranges from +174° to +180°.
• Content
  High-performance liquid chromatography (HPLC): We determine the chromatogram of the sample using octadecylsilane-bonded silica gel as filler and water-methanol as mobile phase. Immediately afterward, we calculate the content of γ-CD in the samples based on the area of the peaks in the chromatograms using the external standard method.
• pH
  An appropriate amount of saturated potassium chloride solution is added to an aqueous solution of γ-CD and the pH is checked using a pH meter. The pH of γ-CD should be 5.0 to 8.0.
• Reducing glycans
  An alkaline copper tartrate solution is added to the γ-CD solution and heated to boiling. It is then titrated with a potassium permanganate titrant while still hot, and the amount of potassium permanganate used gives the amount of reducing glycans in γ-CD.
• Loss on drying
  γ-CD is dried to constant weight at 105°C with a weight loss of not more than 11.0%.
• Scorch residue
  The residue of γ-CD should not exceed 0.1% by autoclaving.
• Microbiological limits
  We use the microbial counting method to detect the number of microorganisms in γ-CD. The total number of aerobic bacteria should not exceed 10³ cfu and the total number of moulds and yeasts should not exceed 10² cfu per 1 g of γ-CD. It is important to note that Escherichia coli should not be present in γ-CD.

Publication Data

Frequently Asked Questions

• What are the methods of preparation of γ-CD?
  Microbial fermentation method: suitable microorganisms are cultured together with a sugar substrate (e.g. starch or glucose) to synthesize γ-CD by using the enzymatic activity of the microorganisms.
  Chemical synthesis method: γ-CD is synthesized by conversion and high-temperature reaction under suitable reaction conditions.
• What are the structural characteristics of γ-CD?
  γ-CD as a whole exhibits a ring-like structure with a cavity at the center. It is hydrophobic with internal -CH- and glucoside-bound oxygen atoms, and hydrophilic with -OH groups at the 2-, 3- and 6-positions of the glucose, which can complex other molecules (examples include rare gases, halogens, pharmaceuticals, foodstuffs, etc.) into inclusion by weak van der Waals forces.

Advantages

• We have cutting-edge pharmaceutical analysis technology and a professional research team to provide custom experimental solutions.
• We provide comprehensive experiment reports and data analysis for our clients.
• We provide 24-hour after-sales service to ensure that our clients complete their projects successfully.

CD BioGlyco has a wealth of successful experience in Pharmaceutical and Biological Analysis, and our professional services have brought convenience to clients all over the world. Client satisfaction has always been our unremitting pursuit, and we are committed to becoming our clients' most capable assistants. Please feel free to contact us if you are interested in our services.