POS consists of 3-10 or up to 20 monosaccharide residues or even disaccharides, with low molecular weight. POS is formed by linking monosaccharide units through a glycosidic bond between the anomeric carbon of one sugar and any unmodified hydroxyl group in another monosaccharide. POS is linked to lipids and amino acids by way of O-glycosidic and N-glycosidic bonds to produce glycolipids and glycoproteins.
Fig.1 POS structure. (Zheng, et al., 2021)
POS can be generated from pectin by enzymatic hydrolysis, which selectively cleaves the glycosidic bonds of pectin into shorter chains of POS. POS has been demonstrated to have various bioactive properties, including antioxidant, prebiotic, and immunomodulatory properties. POS exerts significant identity in cell communication, inhibition of gastrointestinal pathogen adhesion, and stimulation of apoptosis in human colon adenocarcinoma cells.
Based on advanced technologies, CD BioGlyco provides various Oligosaccharide Analysis Services to meet the specific requirements of our global clients. Due to the complex chemical composition of pectin and the chemical alteration during POS production, the POS structure is complicated. The complex mixtures of POS are analyzed using high-sensibility and ability methods. Various analytical techniques have been used to determine the structure and chemical composition of POS originating from different pectin.
HPLC is at present the main analytical technique used for carbohydrates analysis.
Complex mixtures of oligosaccharides are separated and analyzed efficiently by RP-HPLC.
HPLC-PAD is used to analyze quantitatively after released by hydrolysis.
MS and MALDI-MS are used to classify oligosaccharides degraded from pectin.
For example, we characterize POS obtained from citrus peel pectin. Our process is as follows:
Sample preparation: Preprocessing of citrus peel pectin samples.
Enzymatic hydrolysis of citrus peel pectin: Citrus peel POS is obtained by enzymatic hydrolysis.
Fig.2 Characterization of POS obtained from citrus peel pectin. (CD BioGlyco)
CD BioGlycohas developed cutting-edge solutions with advanced technologies and experienced scientists. We are pleased to meet every specific technical or research requirement of our global clients. Please feel free to contact us for more information.