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Glycophage Display

Client Satisfaction with Glycophage Display ls Our Pursuit

With our specialized Glycan Display Platform and experienced staff, CD BioGlyco offers an all-in-one solution for rapid, accurate, and high-throughput screening of glycophage display. Engineered glycophages based on surface patterns display unique carbohydrate epitopes. Notably, the glycans displayed by the phage retain their biological activity after immobilization and can be identified by different glycan-binding proteins (e.g. serum antibodies, lectins, etc.).

Fig.1 Schematic diagram of glycophage display services. (CD BioGlyco)

Our company provides constructed glycophages, modified glycophages (such as phosphorylation), and a series of analysis and detection services according to the needs of our clients. The project service report contains service processes, construction routes, raw maps, and analysis data. We are very concerned about the consistency and effectiveness of the service, please rest assured to cooperate with us!

Fig.2 Flow chart of glycophage display. (CD BioGlyco)

Glycophage Display Services at CD BioGlyco

At CD BioGlyco, we mainly provide two glycoprotein-based glycophage display system construction services

N-linked Glycoprotein-based Glycophage Display System Construction Service

N-linked glycosylation is one of the post-translational modifications of proteins. The N-glycans are covalently bound to the nitrogen atoms of the asparagine residues of the protein by glycosidic bonds. Our researchers have successfully assembled N-linked glycoproteins on phage particles by phage infection of glycosylated E. coli. In addition, we provide detailed analytical services for engineered bacterial N-linked glycosylation pathways.

O-linked Glycoprotein-based Glycophage Display System Construction Service

O-linked glycosylation is the process by which the GalNAc monosaccharide is linked by an α-bond to a serine (Ser) or threonine (Thr) residue on the backbone of the protein. Our investigators provide comprehensive services to clients by constructing O-glycophage libraries, optimizing protein expression, purifying glycophages, and analytical screening. Moreover, we provide detailed analytical services for engineered bacterial O-linked glycosylation pathways.

Specifically, we showed enrichment of proteins with binding specificity or other activities by M13 phage. To further explore the glyco-protein interaction function and extend the diversity of libraries, we provide glycan array and the following several high-quality services.

Glycophage Display-based Glycosylase Genetic Analysis Service

Our team provides effects of glycosylase function and altered or directed modification of glycosylase activity services. In addition, CD BioGlyco uses glycophage recognition residue sites to analyze the role of association between phage-associated glycan epitopes and phage-encoded genes.

Glycophage Display-based Glycoarray Service

Based on glycophage display, CD BioGlyco offers glycoarray fabrication and a validated glycophage printing service. This helps to increase the diversity of glycophage display libraries and provides high-throughput analytical tools for molecular interaction and functional studies.

Glycophage Display-based Antibody Development Service

CD BioGlyco utilizes a large phage antibody library to provide phage antibody array development service. We provide specific antibody-displaying phage sensor preparation, label modification (e. g. Cy3), and fluorescence spectroscopy services. These services provide a useful tool for the diagnosis of diseases and the screening of new drugs.

Glycophage Display-based Epitope Mapping Service

With the support of glycophage display, CD BioGlyco systematically dissects the antigen-antibody interactions by analyzing the binding site (epitope) of the antibody on the antigen. Moreover, we provide individual immunoassay services for individual viral infections.

Glycophage Display-based Biomarker Development Service

Our researchers use glycophage display to screen for and identify molecules (such as peptides, and proteins) that specifically bind to cancer markers. This service plays a key role in early cancer detection.

Publication

Technology: Western blot, DNA sequencing, PCR

Journal: Protein Science

Results: In this study, researchers have developed an M13 phage display system that displays N-glycoprotein. The system was obtained by fusing the N-glycoprotein with the phage shell protein g3p. It provides a new genetic pathway for bacterial N-glycosylation analysis. After multiple analyses, it was shown that both glycol-epitopes and coding genes of the phage establish genotype-phenotype linkage.

Fig.3 Enrichment of glycosylated phage by soybean agglutinin panning. (Celik, et al., 2010)

Applications of Glycophage Display

Glycoprotein-based glycophage display system is an invaluable tool for the selection of glyco-phenotypes.
Glycophage display plays an important role in the glycoengineering of E. coli and in studying the genetic mechanism of protein glycosylation.
Glycophage display is a novel research target for proteomics and glycomics.
Glycophage display detects unique glycan biomarkers of severe metabolic diseases and signature elements of most bacterial and viral pathogens.
Glycophage display is a key mechanism for elucidating mammalian biology, host-pathogen interactions, and immune recognition of glycans.
M13 phage display is an effective isolation tool that can be applied to isolate peptide and protein mutants in a large number of clones.
Glycophage display dissects the complex biological pathways that execute specific processes.
Glycophage display can be used for display antibody clones for detection of leukocyte surface antigens, and small peptides for diagnosis of immune responses in cancer patients.

CD BioGlyco provides a professional glycan display platform for studying the interaction of carbohydrates with multiple biomolecules. Our rich glycan quantities and diverse structures help clients overcome the critical challenges of array manufacturing. To learn more information about glycophage display, please feel free to contact us.

References

Celik, E.; et al. A filamentous phage display system for N-linked glycoproteins. Protein Sci. 2010, 19(10): 2006-13.
Dürr, C.; et al. The Escherichia coli glycophage display system. Glycobiology. 2010, 20(11): 1366-72.
Celik, E.; et al. Glycoarrays with engineered phages displaying structurally diverse oligosaccharides enable high-throughput detection of glycan-protein interactions. Biotechnol J. 2015, 10(1): 199-209.