The active ingredient of lactitol is lactitol monohydrate, which is a monosaccharide derivative formed by linking galactose and sorbitol through glycosidic bonds. The chemical name of lactitol is 4-O-β-D-galactopyranosyl-D-glucitol. Lactitol is an oral osmotic laxative, which is rarely absorbed systemically and is not absorbed in the intestine. It is metabolized by colonic microorganisms into D-galactose and D-sorbitol, which are fermented into organic acids, containing lactic acid, formic acid, propionic, butyric, and acetic acids. Through osmosis, extra fluid is transported to the colon, thereby promoting intestinal peristalsis, and acting as a laxative. Moreover, studies have shown that supplementing lactitol also improves the composition of fecal flora and increases the content of Bifidobacteria.
Analysis of lactitol is critical as it is widely applied in the food and pharmaceutical industries. CD BioGlyco provides lactitol analysis services for our clients worldwide by the following methods.
Fig.1 Lactitol analysis service. (CD BioGlyco)
Paper Title: Determination of maltitol, isomaltitol, and lactitol by high-pH inion-exchange chromatography with pulsed amperometric detection
Journal: Journal of agricultural and food chemistry
Results: HPAEC-PAD is a very powerful tool for the test of sugars and sugar alcohols. The authors developed an optimized HPAEC−PAD method using a thin-film anion-exchange column with a low ion-exchange capacity to quickly and efficiently separate sugar alcohols, containing lactose, present in many commercially available nutraceuticals using a highly basic mobile phase. Lactitol and other sugar alcohols were rapidly separated under optimized conditions without the need for strong base addition to the eluent post-column and without column regeneration between runs. This way is simple, sensitive, and reproducible.
Fig.2 Separation in HPAEC with integrated PAD of a mixed standard solution containing: lactitol. (Cataldi, et al., 1999)
CD BioGlyco has accumulated rich experience in the field of sugar analysis and provides professional lactitol analysis services in different samples. If you need high-quality lactitol analysis services, we are your first choice. Please feel free to contact us for more details.