Based on our comprehensive carbohydrate metabolism analysis solutions, CD BioGlyco provides raffinose analytical services in beets, malt, and peas for our clients.
Raffinose, also known as Melitose or Melitriose, is a non-reducing trisaccharide composed of galactose, glucose, and fructose linked by α- and β-glycosidic bonds. It is an important component of the raffinose family of oligosaccharides (RFO), which is present in a variety of vegetables and grains, such as broccoli, asparagus, beets, and beans. α-Galactosidase can hydrolyze raffinose to D-galactose and sucrose. In some plants, raffinose can be used as a form of carbohydrate storage and transport. In addition, Raffinose can be used in food and health products. For example, raffinose is used as the prebiotic to promote the growth of Lactobacillus and Bifidobacterium.
Fig.1 Raffinose structure. (Wikipedia)
RFOs such as raffinose and stachyose are synthesized by a unique set of galactosyltransferases that in turn add galactose units to sucrose. In plants, raffinose is hydrolyzed by α-galactosidase to produce galactose. Galactose is phosphorylated by galactokinase to produce galactose-1-phosphate. Galactose-1-phosphate is further metabolized by uridine diphosphate (UDP)-galactose to produce UDP-glucose or glucose-1-phosphate. In some plants, raffinose constitutes an important form of carbon transport, and its accumulation is closely related to the response to environmental factors, such as cold, heat, and drought stress.
Fig.2 Rafﬁnose synthesis pathway. (Dobrenel, 2013)
At CD BioGlyco, we have developed complete Carbohydrate Metabolism Analysis solutions, providing rafﬁnose analysis services in beets, malt, and peas for our clients. Our methods include:
HPLC is the most common chromatographic technique for the determination of sugar content. We can determine rafﬁnose content in beets, malt, and peas by HPLC with refractive index detection (HPLC-RID), HPLC with evaporative light scattering detector (HPLC-ELSD), and HPLC-mass spectrometry (HPLC-MS).
HPAEC-PAD is mainly used for rafﬁnose analysis in samples containing various carbohydrates.
In addition, we can also provide customized solutions based on your specific needs.
CD BioGlyco has been committed to carbohydrate metabolism research for many years and accumulated rich experience. We provide accurate, reliable, and reproducible raffinose analysis services for our clients worldwide. If you are interested in our services, please feel free to contact us, we are looking forward to being your research assistant in the field of carbohydrate metabolism analysis.