Esterified Fatty Acid Analysis

Esterified Fatty Acid Analysis

Fatty acids (FAs) play an important role in the human body and are used as biomarkers in the early stages of various pathological development. CD BioGlyco has been committed to FAs analysis and research for many years and has been greatly recognized internationally. We provide customers with analysis services of esterified fatty acids (EFAs) in diverse samples by gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detector (GC-FID).


Fat is composed of a complex mixture of FAs. FAs are essential nutrients for the human body and an important component of tissue cells. FAs include saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs), and polyunsaturated fatty acids (PUFAs). SFAs affect blood lipids. MUFAs have the effects of lowering blood sugar, regulating blood lipids, and lowering cholesterol. PUFAs have important regulatory effects on the structure and physiological functions of various tissues in the body.

In human blood, FAs exist in various compounds in a combined form, collectively referred to as lipids. In addition to EFAs, there are also non-esterified fatty acids (NEFAs) or free fatty acids (FFAs) in the blood. EFAs and NEFAs are metabolized through different pathways and play different roles. Synthesis of NEFAs produces EFAs and decomposition of EFAs produces NEFAs. FAs are used as biomarkers in the early stages of various pathological development. When studying the role and metabolism of FAs in the body, it is necessary to quantitatively analyze the free and bound forms of FAs present in biological samples.

A separation scheme for the plasma sample pretreatment.Fig.1 A separation scheme for the plasma sample pretreatment. (Han, 2011)


As a leader in the field of fatty acid analysis, CD BioGlyco provides customers with high-quality EFA analysis services. Our analysis methods include but are not limited to GC-MS and GC-FID.

Case: Plasma EFAs and NEFAs metabolic profiling by GC-MS

CP 3800-Saturn 2200 GC-MS instrument; VF-5ms capillary column; 200°C ion source temperature; Temperature rose 90°C for 1 min ~ to 212°C at 15°C/min ~ 212°C for 2 min ~ to 219°C at 0.8°C/min ~ 219°C for 2 min ~ to 290°C at 25°C/min ~ 290°C for 5 min; 280°C injection temperature; 1.0 mL/min flow rate; 2.0 µL samples; Electron ionization (EI).

Advantages of Us

  • High resolution, high sensitivity, and high accuracy
  • Complete analysis report
  • One-stop EFAs analysis service
  • 24h after-sales service


  • Identification of lipid components in food
  • Analysis of aviation kerosene
  • Research of clinical metabolomics

After years of research, CD BioGlyco has developed a complete solution for Fatty Acid Analysis. We are very pleased to share our knowledge and experience. If you are interested in this field, please feel free to contact us. We will do our best to meet your needs.


  1. Han, L.D.; et al. Plasma esterified and non-esterified fatty acids metabolic profiling using gas chromatography-mass spectrometry and its application in the study of diabetic mellitus and diabetic nephropathy. Analytica Chimica Acta. 2011, 689(1): 85-91.
This service is for Research Use Only, not intended for any clinical use.

About Us

CD BioGlyco is a world-class biotechnology company with offices in many countries. Our products and services provide a viable option to what is otherwise available.

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