Chlorhexidine Gluconate Analysis Service

Chlorhexidine Gluconate Analysis Service

Precision and Expertise in Chlorhexidine Gluconate Analysis

Chlorhexidine gluconate is a bisguanidine chlorobenzene with a broad spectrum of antimicrobial activity. Chlorhexidine gluconate is also a powerful disinfectant and antimicrobial agent and can be prepared as a solution, gargle, or topical solution for a wide range of pharmaceutical applications. At CD BioGlyco, we provide high-quality chlorhexidine gluconate analysis services with extensive experience in the field of Pharmaceutical Analysis. The services we offer include but are not limited to the following.

  • State
    Chlorhexidine gluconate is a clear, colorless slight yellow, and slightly viscous liquid. It is miscible with water and soluble in ethanol or acetone.
  • Identification
    • We dilute the sample well with water and copper sulfate solution, then, the solution is boiled to cause the precipitate to coalesce. If a lavender color appears in the solution, the sample is chlorhexidine gluconate.
    • Add ferric chloride solution to an aqueous solution of the sample and heat to boiling. If the solution changes color to yellow, the sample is chlorhexidine gluconate.
    • The sample is added to a hot solution of cetyltrimethylammonium bromide to which a bromine test solution is added along with a sodium hydroxide test solution. If the solution shows a deep red color, the sample is chlorhexidine gluconate.
  • Acidity
    The acidity of chlorhexidine gluconate is tested using a pH meter.
  • Detection of p-chloroaniline
    We dilute chlorhexidine gluconate well with water, hydrochloric acid, and ethanol and compare the color of the solution with that of a p-chloroaniline solution against a black background. If the colors are identical, p-chloroaniline is present in chlorhexidine gluconate.
    • Impurity assay
      A sample of chlorhexidine gluconate is added to the acetic acid solution. Next, the solution is unfolded on the same thin-layer silica gel plate as the solution of chlorhexidine gluconate standard according to wave-layer chromatography. Finally, the thin layer plate is placed under an ultraviolet light for review. If the sample contains stray dots it means that the sample contains impurities.
    • Content assay
      Chlorhexidine gluconate is weighed accurately and diluted with water. Then, we add ethanol solution to it. The absorbance is measured spectrophotometrically. Finally, we calculate the content of chlorhexidine from the absorbance value.

Fig.1 Chlorhexidine gluconate analysis services. (CD BioGlyco)Fig.1 Chlorhexidine gluconate analysis services. (CD BioGlyco)

Publication Data

Technology: In vivo and in vitro experiments

Journal: Pharmaceutics

IF: 5.4

Published: 2022

Results: In this article, the authors researched the in vivo bioactivity of chlorhexidine gluconate to understand the time required for chlorhexidine gluconate to form a film, the appearance of the film, and its adhesion to the skin. The authors also researched the antimicrobial activity of chlorhexidine gluconate in vitro and in vivo and evaluated optimized formulations for cytotoxicity and wound healing in vitro cell line evaluations. The results showed that transparent homogeneous solutions of chlorhexidine gluconate yielded transparent and pliable films upon actuation and were effective in inhibiting the growth of Staphylococcus aureus in vitro.

Fig.2 Application of chlorhexidine gluconate sprays. (Chamsai, et al., 2022) Fig.2 Application of chlorhexidine gluconate sprays. (Chamsai, et al., 2022)


  • A comprehensive analysis of chlorhexidine gluconate can be used for research on the composition of medicinal antimicrobial agents.
  • The analysis of chlorhexidine gluconate can be used to investigate its metabolic pathway in the human body.
  • The analysis of chlorhexidine gluconate can be used to investigate the amount of chlorhexidine gluconate in pharmaceuticals.


  • We provide custom pharmaceutical analysis services to our clients with our best-in-class Pharmaceutical and Biological Analysis technology.
  • We provide complete pharmaceutical analysis reports to our clients.
  • Our analysis techniques are characterized by high resolution, high sensitivity, and high accuracy to ensure that the needs of our clients are met.

CD BioGlyco has deep experience in the field of pharmaceutical analysis and has developed complete solutions for pharmaceutical analysis. If you would like to learn more about pharmaceutical analysis, please feel free to contact us.


  1. Chamsai, B.; et al. Topical film-forming chlorhexidine gluconate sprays for antiseptic application. Pharmaceutics. 2022, 14(6): 1124.
This service is for Research Use Only, not intended for any clinical use.

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