Phosphosphingolipid Analysis Service

CD BioGlyco has developed an advanced one-stop solution for lipidomics analysis. We are committed to providing clients with high-quality analysis services for ceramide, cerebroside, diglycosylceramide, globoside, ganglioside, sulfoglycosphingolipid, and phosphosphingolipid.

Overview of Phosphosphingolipid

Sphingolipid is a class of amphoteric lipids containing a sphingosine backbone that was discovered in brain extracts in the 1870s. It generally exists in plant and animal membranes, especially in the tissues of the central nervous system. Phosphosphingolipid is a sphingolipid consisting of a ceramide backbone attached to a phosphorylated "head" group, found in animals, plants, bacteria, and fungi. Here, we will introduce 5 phosphosphingolipids.

  • Sphingomyelin (SM)
    SM is the most common sphingolipid, composed of ceramide and phosphorylcholine. It is present in the plasma membrane of most mammalian cells and is a major component of myelin. The metabolites of SM are important signaling molecules that regulate signaling processes such as cell growth, differentiation, senescence, and apoptosis. In addition, abnormal metabolism of SM is associated with Alzheimer's disease and cardiovascular disease.
  • Ceramide phosphoethanolamine (CPE)
    CPE is composed of a ceramide backbone bound to phosphoethanolamine molecules. It is the major sphingolipid in arthropod cell membranes. Moreover, CPE is also contained in invertebrates such as bees and spiders. Although the existence of CPE has been found in multiple species, more efforts are still needed to explore its molecular functions in living organisms.
  • Inositol phosphorylceramide (IPC)
    IPC is a phosphosphingolipid coupled by a phosphoinositide head group to a ceramide. It was originally identified from Saccharomyces cerevisiae as a major component of yeast biofilms. IPC was later found in other yeasts, protozoa, plants, and detergent-resistant films of several organisms. In lipid rafts of cell membranes, IPC interacts with membrane proteins with signaling functions.

Fig.1 Sphingolipid metabolic pathways in yeast and mammals.Fig.1 Sphingolipid metabolic pathways in yeast and mammals. (Matmati & Hannun, 2008)

  • Mannosylinositol phosphateceramide (MIPC)& M(IP)2C
    MIPC is a phosphosphingolipid formed by the coupling of mannosyl phosphoinositide molecules with ceramides. IPC, MIPC, and mannosyldiinositol phosphorylceramide [M(IP)2C] together constitute the major sphingolipids in yeast. They are intermediates and products of common biosynthetic pathways. In cells, they are involved in the localization of cell membrane proteins and maintain cell morphology.

Due to the important functional and biological significance of phosphosphingolipid, it is highly necessary to develop rapid and accurate analytical techniques.

Phosphosphingolipid Analysis Service

Mass spectrometry (MS) is increasingly used in lipidomic analysis. At CD BioGlyco, we have developed a high-sensitivity, reproducible, high-resolution, and high-accuracy method for sphingolipid analysis. We provide global clients with analysis services for SM, CPE, IPC, MIPC, and M(IP)2C in different samples by Q Exactive Orbitrap-MS. We will define the content of SM, CPE, IPC, MIPC, and M(IP)2C in pmol and mol%. After the project is completed, we will provide a complete project report, including sample preparation, analytical methods, instrument parameters, analytical data, and spectrum.

Type Substances Method Ranges
Phosphosphingolipid SM Q Exactive Orbitrap-MS
  • Total length from 30 to 46
  • Total DB from 0 to 2
  • Total OH from 2 to 4
CPE Q Exactive Orbitrap-MS
  • Total length from 30 to 46
  • Total DB from 0 to 2
  • Total OH from 2 to 4
IPC Q Exactive Orbitrap-MS
  • Total length from 36 to 48
  • Total DB from 0 to 1
  • Total OH from 3 to 6
MIPC Q Exactive Orbitrap-MS
  • Total length from 36 to 48
  • Total DB from 0 to 1
  • Total OH from 3 to 6
M(IP)2C Q Exactive Orbitrap-MS
  • Total length from 36 to 48
  • Total DB from 0 to 1
  • Total OH from 3 to 6

Advantages of Us

  • Comprehensive solution for lipidomic analysis
  • High-sensitivity and reproducible
  • High-resolution and high-accuracy
  • Experienced scientist

CD BioGlyco has extensive experience in lipidomics and has developed various methods for lipid analysis. We are willing to share our knowledge and experience in this field. If you are interested in our services, please contact us for more details.

Reference:

  1. Matmati, N.; Hannun, Y.A. ISC1 (inositol phosphosphingolipid-phospholipase C), the yeast homologue of neutral sphingomyelinases. J. Lipid Res. 2008, 49: 922-928.
This service is for Research Use Only, not intended for any clinical use.

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