Isepamicin Sulfate Analysis Service

Isepamicin Sulfate Analysis Service

Uncovering Precise Isepamicin Sulfate for Better Tomorrow

Isepamicin sulfate is an aminoglycoside that can effectively inhibit bacterial protein synthesis. It is often used as an antimicrobial agent because of its good antibacterial function. CD BioGlyco has been committed to research in the field of glycobiology and has rich knowledge and experience in the analysis of Pharmaceuticals. With our leading Pharmaceutical and Biological Analysis technology, we provide our clients with dedicated isepamicin sulfate analysis services.

Fig.1 Isepamicin sulfate analysis services. (CD BioGlyco)Fig.1 Isepamicin sulfate analysis services. (CD BioGlyco)

  • Physical and chemical properties
    Isepamicin sulfate appears as a white or off-white powder. Isepamicin sulfate is easily soluble in water, however, it is not soluble in methanol and ethanol.
  • Specific rotation
    We measure the specific rotation of isepamicin sulfate using a spinometer. The specific rotation of isepamicin sulfate is usually between +100° and +120°.
  • pH
    The pH of isepamicin sulfate is tested using a pH meter. The pH of isepamicin sulfate is in the range of 5.5-7.5.
  • Identification
    • The sample to be tested is dissolved in water to which an anthrone solution is added. The color of the solution is used to identify the sample as isepamicin sulfate.
    • We will dissolve the sample to be tested in water, to which barium chloride solution is added. When a white precipitate appears, hydrochloric or nitric acid is added. If the precipitate no longer dissolves, the sample is isepamicin sulfate.
  • Content assay
    High-performance liquid chromatography (HPLC): The aqueous solution of isepamicin sulfate is subjected to gradient elution using trifluoroacetic acid and methanol as mobile phases. Finally, the amount of isepamicin sulfate is obtained from the retention time of the main peak of isepamicin sulfate.
  • Sulfate content
    First, isepamicin sulfate is accurately weighed and dissolved in water. Then, the chromatogram of isepamicin sulfate is tested according to liquid chromatography. Finally, the amount of sulfate is calculated based on the area of the peaks in the chromatogram.
  • Moisture content
    A mixture of formamide-anhydrous methanol is used as a solvent to dissolve isepamicin sulfate, which is then assayed for moisture content using volumetric titration.
  • Cauterization of residue
    After weighing isepamicin sulfate, we place it in a crucible. Then, it is heated until it is completely charred. Finally, it is transferred to a desiccator and then it is weighed to get the residue weight of isepamicin sulfate.
  • Abnormal toxicity test
    First, isepamicin sulfate is dissolved in sodium chloride injection, and then the resulting solution is injected intravenously into mice. If the mice die within a specific time, isepamicin sulfate is abnormally toxic.
  • Antihypertensive substance
    We inject a sodium chloride solution of isepamicin sulfate into an animal and the blood pressure of the animal before and after the injection will be recorded. The changes in blood pressure are analyzed to determine whether isepamicin sulfate contains antihypertensive substances.

In addition, isepamicin sulfate injection, a sterilized aqueous solution of isepamicin sulfate, is also a common form in the field of biological research, and we follow the analysis method of isepamicin sulfate and still provide isepamicin sulfate injection analysis to our clients.

Publication Data

Technology: Reversed-phase HPLC

Journal: Journal of Pharmaceutical and Biomedical Analysis

IF: 3.4

Published: 2001

Results: The authors used reversed-phase HPLC to analyze isepamicin sulfate. Wherein, the authors used a set of tandem columns to separate and purify isepamicin sulfate at 10°C. Then, the impurity analysis of isepamicin sulfate was also evaluated using different standard curves. For example, a standard curve of 1.6 mg/ml can be used to analyze the amount of isepamicin sulfate. Whereas, a standard curve of 8.0 mg/ml can be used to detect impurities of isepamicin sulfate.

Frequently Asked Questions

  • How is isepamicin sulfate prepared?
    Isepamicin sulfate is a new semi-synthetic aminoglycoside antibiotic, usually prepared from gentamicin B protected by tertiary silica ethoxycarbonyl chloride and then attached to isoserine. Alternatively, gentamicin B is chelated with zinc ions, amidated, and then attached to isoserine. Because gentamicin B is an efficient source for the preparation of isepamicin sulfate, it is often necessary to detect the presence of the impurity gentamicin in the analysis of isepamicin sulfate.
  • What is the function of Isepamicin sulfate?
    Isepamicin sulfate, an aminoglycoside, is mainly used for sepsis caused by sensitive bacteria, secondary infections caused by trauma, burns, surgery, chronic bronchitis, bronchiectasis, pneumonia, pyelonephritis, cystitis, and peritonitis. Sensitive bacteria include many types, such as Escherichia coli, Citrobacter, Klebsiella Serratia, etc.


  • We develop the most appropriate analysis protocol based on the type of pharmaceutical or use a combination of analysis techniques for a comprehensive analysis of the pharmaceutical.
  • We offer analysis of all types of glycan drugs using analysis techniques relevant to the fields of chemistry and biology.
  • We provide timely feedback to our clients on the progress of their pharmaceutical analyses to ensure their full participation.

CD BioGlyco's professional pharmaceutical analysis team is dedicated to helping our clients solve the challenges they encounter in the field of pharmaceutical research. We have efficient pharmaceutical analysis solutions and reliable pharmaceutical analysis researchers. If you want to know more about pharmaceutical analysis, please feel free to contact us.


  1. Vogel, R.; et al. Determination of isepamicin sulfate and related compounds by high-performance liquid chromatography using evaporative light scattering detection. Journal of Pharmaceutical and Biomedical Analysis. 2001, 24(3): 405-412.
This service is for Research Use Only, not intended for any clinical use.

About Us

CD BioGlyco is a world-class biotechnology company with offices in many countries. Our products and services provide a viable option to what is otherwise available.

Contact Us

Copyright © CD BioGlyco. All rights reserved.