Glycosylation is present on 50% of proteins and is considered an important post-translational modification. Protein glycosylation plays a key role in many biological processes, such as cell adhesion, protein translation regulation, cellular immunity, protein degradation, etc. The glycosylation pattern changes during tumor development, which can be used for early cancer diagnosis and monitoring the progress of tumorous diseases. Glycoproteomics is a branch of proteomics used to identify, classify, quantify, and characterize glycoproteins, which includes the elucidation of glycosylation sites and the analysis of glycoprotein structure and function.
The comprehensive analysis of glycoproteins is particularly challenging due to the structural diversity of glycans and the heterogeneity of glycosylation sites. In recent years, the rapid development of mass spectrometry (MS) instruments, fragmentation strategies, and high-throughput workflows has made it possible to analyze intact glycoproteins. Several enrichment methods can detect even low abundances of glycans and glycopeptides, and various experimental workflows tailored for the quantification, site occupancy and structural analysis of glycoproteins have been developed, resulting in the rapid development of glycoproteomics.
Fig.1 Glycomics/glycoproteomics workflow for the analysis of a purified glycoprotein. (Yang, 2017)
In order to overcome the challenge of glycoprotein analysis, we provide a variety of enrichment and purification methods to improve the detection sensitivity.
In a variety of basic sciences and applied sciences, quantification of oligosaccharides carried by proteins has become increasingly important.
The properties of glycoproteins are largely affected by site occupation. To further study the microheterogeneity of glycoproteins, we provide site occupancy analysis.
We provide advanced technology platforms such as MALDI-TOF and HILIC for the overall analysis of the complex structure of glycoproteins and their derivatives.
CD BioGlyco offers our customers N-glycoproteomics analysis in PDX models, which is helpful to the development of personalized cancer treatment.
CD BioGlyco offers several nanotechnologies for the detection of glycopeptides in complex samples with high sensitivity and specificity.
CD BioGlyco has rich experience in glycoproteomics analysis. Our well-trained researchers provide a variety of high-quality analysis services according to your research needs. We hope to start the exploration of glycobiology with you. If you are interested in our services, please contact us for more detailed information.