Ganglioside Analysis Service

CD BioGlyco has developed a complete lipidomics analysis solution for clients. We provide fast, efficient, and accurate ganglioside analysis services by mass spectrometry (MS). Welcome global clients to establish cooperation with us!

Structures and Metabolism of Gangliosides

Gangliosides are composed of acidic glycosphingolipids with one or more N-acetylneuraminic acid (NANA) residues attached to the sugar chain. In 1942, German scientist Ernst Klenk first named gangliosides for lipids isolated from ganglion cells in the brain. Gangliosides are the structural components of cell membranes, with their two hydrocarbon chains embedded in the plasma membrane, while oligosaccharides are located on the extracellular surface. They provide recognition points for extracellular substances or surface molecules of adjacent cells and play an important role in cell recognition, adhesion, and signaling transmission.

Gangliosides are a complex class of lipids that are synthesized primarily in the endoplasmic reticulum (ER), and then further modified in the Golgi by the sequential addition of additional carbohydrates. Except monosialogangliosides 4 (GM4) derived from galactosylceramide (GalCer), most gangliosides are synthesized from lactosylceramide (LacCer). The gangliosides derived from LacCer are divided into asialo-series gangliosides, a-series gangliosides, b-series gangliosides, and c-series gangliosides according to the number of sialic acid residues linked to the internal galactose (Gal) residues.

Fig.1 Structures and biosynthetic pathways of gangliosides.Fig.1 Structures and biosynthetic pathways of gangliosides. (Robert, et al., 2011)

Functions of Ganglioside

Gangliosides are found in most mammalian tissues and are most abundant in the nervous system. Their unique physical properties make them important parts of the cell membrane, involved in cell recognition and cell communication. Studies have shown that gangliosides are essential for neuronal function and brain development. They provide neuroprotection by promoting neurodevelopment in newborns and are involved in memory and learning in the brain. Furthermore, gangliosides have been implicated in the pathology of many diseases, such as Guillain-Barré syndrome, which is caused by an autoimmune response to cell surface gangliosides. Alzheimer's disease is also thought to be initiated by the aggregation of amyloid-β peptides caused by gangliosides.

Ganglioside Analysis Service

At CD BioGlyco, we have developed a high-sensitivity, reproducible, high-resolution, and high-accuracy solution for sphingolipid analysis. We provide GM, disialogangliosides (GD), trisialogangliosides (GT), and tetrasialogangliosides (GQ) analysis in various sample types by Q Exactive Orbitrap-MS. Gangliosides we analyze include but are not limited to GM1, GM2, GM3, GM4, GD1, GD2, GD3, GT1, GT2, GT3, and GQ1.

Type Substance Method Ranges
GM GM1 Q Exactive Orbitrap-MS
  • Total length from 30 to 46
  • Total DB from 0 to 2
  • Total OH from 2 to 4
GM2 Q Exactive Orbitrap-MS
GM3 Q Exactive Orbitrap-MS
GM4 Q Exactive Orbitrap-MS
GD GD1 Q Exactive Orbitrap-MS
  • Total length from 30 to 46
  • Total DB from 0 to 2
  • Total OH from 2 to 4
GD2 Q Exactive Orbitrap-MS
GD3 Q Exactive Orbitrap-MS
GT GT1 Q Exactive Orbitrap-MS
  • Total length from 30 to 46
  • Total DB from 0 to 2
  • Total OH from 2 to 4
GT2 Q Exactive Orbitrap-MS
GT3 Q Exactive Orbitrap-MS
GQ GQ1 Q Exactive Orbitrap-MS
  • Total length from 30 to 46
  • Total DB from 0 to 2
  • Total OH from 2 to 4

In addition, we also provide diverse ganglioside products for global clients, including but not limited to:

Advantages of Us

  • Comprehensive solution for lipidomic analysis
  • High-sensitivity and reproducible
  • High-resolution and high-accuracy
  • Experienced scientist

CD BioGlyco has extensive experience in lipidomics and has developed various lipid analysis methods. We are willing to share our knowledge and experience in this field. If you are interested in our services and products, please contact us for more details without any hesitation.

Reference:

  1. Robert, K.Y.; et al. Structures, biosynthesis, and functions of gangliosides-an overview. Journal of oleo science. 2011, 60(10): 537-544.
This service is for Research Use Only, not intended for any clinical use.

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