The central law of biology describes the process of extracting genetic information from genes and making proteins to produce biological effects, i.e., DNA transcription to produce RNA, which translates and synthesizes proteins, and this is a process known as gene expression.
Glycogene expression profiling measures which glycogenes are being expressed in a cell at a given moment, allowing the detection of thousands of genes or even the entire genome at once. This method measures the mRNA level in cells to reflect the level of gene expression, reflecting the existence state of cells at a specific moment. It is widely used to study the function of glycogenes in diseases and to find biomarkers to discover therapeutic targets for diseases.
Fig.1 Expression levels of glycogenes in normal liver cells and hepatocarcinoma cells measured by RNA sequencing (RNA-Seq). (Angata, et al., 2020)
CD BioGlyco has various technologies for glycogene expression profiling and provides clients with qualitative and quantitative analysis on mRNA composition, offering clear information on gene expression type and abundance. With our assistance, clients smoothly study the function of glycogenes in organisms and their changes in different diseases.
Fig.2 Glycogene expression profiling based on different techniques. (CD BioGlyco)
qRT-PCR has high precision and high detection sensitivity and is commonly considered to be the most reliable method to study gene expression. Even low-expressed glycogenes can be analyzed. CD BioGlyco provides clients with an accurate and highly sensitive glycogene expression profiling service based on the qRT-PCR technique.
dPCR is the third-generation PCR system which achieves the absolute quantification of target molecules and the detection of rare alleles. The determination of the results is independent of the Ct value and not affected by the PCR efficiency. CD BioGlyco has professional dPCR technical processes for glycogene expression profiling.
By designing suitable probes, DNA microarray detects the relative expression profile of each gene. CD BioGlyco has first-class DNA microarray technology to customize hybridization probes for clients to precisely detect glycogene expression levels in samples.
Sanger sequencing is the commonly used DNA sequencing technique with an accuracy rate of 99.99%. CD has professional technical personnel to provide clients with Sanger sequencing-based glycogene expression profiling service in high accuracy.
The rapid development of NGS sequencing, has accelerated the development of genomics and molecular biology. CD BioGlyco offers different NGS sequencing services, including whole-genome sequencing, RNA-sequencing, and CHIP-sequencing.
It is our mission to help clients solve their challenges in the field of glycobiology. From Glycogene Editing Service, Glycogene Delivery Service, and glycogene expression profiling, to Cancer Glycogene Discovery Service, CD BioGlyco provides comprehensive and quality Platforms. If you are curious about our platforms, please do not hesitate to contact us. CD BioGlyco is looking forward to working with you.